Allsheng Auto-Pure 10BS Fully Automated Magnetic Bead-Based Nucleic Acid Extraction System

Overview

The Allsheng Auto-Pure 10BS is a compact, benchtop fully automated nucleic acid extraction system engineered for high-fidelity magnetic bead-based purification in resource-limited, field-deployable, and core laboratory environments. It operates on the principle of solid-phase reversible immobilization (SPRI), leveraging paramagnetic beads functionalized with carboxyl or silane groups to selectively bind nucleic acids under controlled chaotropic salt and pH conditions. Integrated thermal control—via a precision Peltier-heated metal block—enables on-instrument lysis, binding, washing, and elution, while optional post-extraction isothermal amplification (e.g., LAMP, RPA) can be initiated directly within the same tube without manual transfer. Designed as a downscaled evolution of the Auto-Pure 20B and 32A platforms, the 10BS prioritizes operational robustness, minimal footprint (0.12 m²), and compliance-ready architecture for decentralized molecular testing workflows.

Key Features

• Compact benchtop form factor (340 × 350 × 410 mm) optimized for biosafety cabinets, mobile labs, and point-of-collection sites
• Dual-zone thermal module: independent lysis/elution heating (RT–120°C) and dedicated metal bath (RT+5°C–105°C) for integrated amplification setup
• High-precision mechanical actuation: dual-axis linear guide rails and fine-pitch lead screws ensure repeatable magnetic rod movement and minimal cross-contamination risk
• UV-C germicidal irradiation (254 nm) between runs, programmable via touchscreen or PC software, supporting GLP-aligned decontamination protocols
• Barcode-driven workflow: built-in QR code scanner auto-detects Allsheng-certified reagent kits, loading pre-validated protocols without manual selection
• Open-system compatibility: accepts third-party 5-mL magnetic bead kits and custom magnetic rod sleeves (e.g., AS-17041-02), with full parameter override capability (mixing speed, magnet engagement duration, fan cooling intensity)
• Real-time remote monitoring and control via encrypted Wi-Fi or Ethernet using Allsheng’s cross-platform PC desktop application and iOS/Android companion app

Sample Compatibility & Compliance

The Auto-Pure 10BS supports diverse clinical and forensic specimen types—including whole blood (EDTA/K₂EDTA), plasma, saliva, buccal swabs, tissue homogenates, FFPE lysates, and environmental swab eluates—across volume ranges from 50 µL to 5 mL. Its validated performance meets ISO 20387:2018 requirements for biobanking quality management and demonstrates ≥95% positive detection rate at ≥100 genomic DNA/RNA copies per reaction, with inter-well coefficient of variation (CV) consistently below 5% across 10-sample batches. The system logs all critical events—including temperature profiles, magnet cycle counts, UV exposure timestamps, and user authentication—into an encrypted internal SD card, satisfying audit trail requirements under CLIA, CAP, and ISO/IEC 17025 frameworks. Firmware supports configurable electronic signatures and time-stamped activity logs compatible with FDA 21 CFR Part 11–aligned validation packages.

Software & Data Management

Allsheng provides two complementary software layers: a Windows-based PC application for protocol authoring, instrument calibration, firmware updates, and comprehensive audit log export (CSV/PDF); and a mobile-responsive web app accessible via browser or native iOS/Android clients. Both interfaces support role-based access control (admin/operator), version-controlled protocol libraries (up to 100 stored methods), and automatic synchronization of instrument status, error codes, and consumables inventory. Raw thermal and motor event logs are timestamped to the millisecond and exportable with metadata tags (operator ID, sample ID, kit lot number). Optional integration with LIMS via HL7 or RESTful API is supported through middleware configuration.

Applications

• Rapid pathogen nucleic acid isolation for outbreak response (e.g., SARS-CoV-2, influenza A/B, RSV) in field hospitals or border screening units
• Forensic DNA extraction from low-yield, inhibitor-rich evidence (bloodstains, touch DNA, degraded bone samples)
• Plant and animal genomic DNA purification for breeding programs and GMO detection workflows
• Pre-analytical automation in companion diagnostics, enabling seamless handoff to qPCR, digital PCR, or NGS library prep systems
• Biobank accessioning with traceable, reproducible yield metrics across longitudinal sample sets

FAQ

Does the Auto-Pure 10BS require proprietary reagents to operate?
No. While Allsheng-certified kits (e.g., AS-17051-01) enable one-touch barcode activation, the system is fully open—users may configure custom magnetic bead chemistries and adjust binding/wash/elution parameters manually.
Can the metal bath function be disabled during nucleic acid extraction only?
Yes. The thermal block and metal bath operate independently; users may deactivate the metal bath and utilize only the standard heating module for conventional extraction protocols.
Is the UV disinfection cycle validated against specific microbial strains?
The 254 nm UV-C source delivers ≥15 mJ/cm² fluence per cycle, sufficient to achieve ≥4-log reduction of common lab contaminants including Escherichia coli, Staphylococcus aureus, and MS2 bacteriophage, per ISO 15714:2019 test methodology.
What data security measures are implemented for remote monitoring?
All Wi-Fi/Ethernet communications use TLS 1.2+ encryption; device authentication employs certificate-based mutual TLS, and local SD card storage is AES-256 encrypted with write-protection options enabled by default.
How is maintenance and calibration documented for regulatory audits?
The PC software includes a built-in calibration logbook with electronic signature fields, auto-generated PDF reports for temperature uniformity mapping, magnet force verification, and mechanical alignment checks—all compliant with ISO/IEC 17025 clause 6.4.6.