Allsheng Auto-Pure 48 Automated Magnetic Bead-Based Nucleic Acid Extraction System

Overview

The Allsheng Auto-Pure 48 is a dedicated fully automated nucleic acid purification system engineered for high-reproducibility magnetic bead-based isolation of DNA and RNA from diverse biological matrices—including whole blood, serum, plasma, saliva, buccal swabs, tissue homogenates, and cultured cells. It operates on the principle of paramagnetic particle separation under controlled magnetic fields, enabling selective binding, washing, and elution of nucleic acids in a closed, walk-away workflow. Designed for mid-to-high throughput laboratories—including clinical diagnostics, molecular pathology, epidemiological surveillance, and translational research—the system delivers consistent yield and purity across variable sample types and input volumes (200–3000 µL), while maintaining strict contamination control through integrated UV sterilization and sealed reagent handling.

Key Features

• True 48-sample parallel processing using standard 5-mL deep-well plates and disposable 3-mL magnetic rod sleeves—no pipetting or tip waste.
• Eight-position rotating deck with dual independently controllable heating modules (ambient to 120 °C) for precise lysis and elution temperature management.
• Patented automatic magnetic rod sleeve loading/unloading mechanism ensures mechanical repeatability and eliminates operator-induced variability or cross-contamination risk.
• Real-time UV-C irradiation (254 nm) during idle and post-run phases, coupled with internal LED illumination, supports routine decontamination and visual process monitoring.
• Intuitive 7-inch capacitive touchscreen interface with three programmable hardware shortcut keys; supports external mouse operation and full program editing (create, duplicate, rename, delete).
• Onboard storage for up to 100 user-defined protocols; preloaded with 8 optimized templates for common kits (e.g., viral RNA, genomic DNA, FFPE-derived nucleic acids).
• Comprehensive audit trail: timestamped run logs—including start/stop time, temperature profiles, error codes, and reagent lot tracking—are automatically saved to internal SD card in CSV format.
• Standard communication interfaces include USB 2.0, 10/100 Mbps Ethernet, and optional Wi-Fi module—enabling integration with LIMS and laboratory automation systems via HL7 or ASTM E1384-compliant data export.

Sample Compatibility & Compliance

The Auto-Pure 48 accommodates a broad range of primary and processed specimens without requiring manual preprocessing steps. Its open-platform architecture permits use with third-party magnetic bead kits compliant with ISO 13485-manufactured reagents and validated under CLIA, CAP, and ISO/IEC 17025 frameworks. The system meets electromagnetic compatibility requirements per IEC 61326-1 and safety standards per IEC 61010-1. UV irradiance output is calibrated to deliver ≥40 µJ/cm² per cycle at 254 nm—validated per ISO 15883-1 for surface decontamination. All thermal profiles are traceable to NIST-traceable reference thermometers, supporting GLP/GMP documentation needs.

Software & Data Management

The embedded firmware supports full protocol versioning, user role-based access control (administrator, technician, viewer), and electronic signature capability for critical steps. Audit logs record all parameter modifications, login/logout events, and hardware status changes—with immutable timestamps aligned to UTC. Data export functions generate ISO/IEC 17025-compliant reports including extraction efficiency metrics (A260/A280, A260/A230), inter-run CVs, and failure root-cause codes. Optional FDA 21 CFR Part 11 compliance package includes digital signature validation, encrypted database storage, and configurable retention policies.

Applications

• Routine extraction of viral nucleic acids (SARS-CoV-2, influenza, RSV, HIV) for RT-qPCR and sequencing library preparation.
• High-yield genomic DNA isolation from EDTA-blood and formalin-fixed paraffin-embedded (FFPE) tissues for NGS target enrichment.
• Simultaneous co-extraction of total RNA and miRNA from low-input clinical samples (e.g., cerebrospinal fluid, plasma exosomes).
• Automated nucleic acid cleanup following enzymatic reactions (e.g., restriction digests, ligations, PCR cleanup) in core genomics facilities.
• Integration into end-to-end automated workflows—paired with liquid handlers, thermal cyclers, and fragment analyzers—for unattended batch processing of 100+ samples per shift.

FAQ

What types of magnetic bead kits are compatible with the Auto-Pure 48?
The system is compatible with all commercially available magnetic bead-based nucleic acid extraction kits that specify use with 5-mL deep-well plates and 3-mL magnetic rod sleeves—including those from Qiagen, Thermo Fisher Scientific, Takara Bio, and domestic ISO 13485-certified manufacturers.
Does the Auto-Pure 48 support remote monitoring or cloud-based data backup?
Yes—when connected via Ethernet or Wi-Fi, the instrument supports secure HTTP/S API access for real-time status polling and scheduled log exports to network-attached storage or cloud platforms (e.g., AWS S3, Azure Blob) using TLS 1.2 encryption.
How is cross-contamination prevented between runs?
Contamination control is enforced through three redundant layers: (1) UV-C irradiation of the entire workspace between cycles, (2) disposable magnetic rod sleeves used once per run, and (3) positive-pressure airflow management with HEPA-filtered exhaust.
Can the heating modules be programmed independently?
Yes—each of the two heated positions supports independent temperature setpoints, ramp rates, and dwell times, allowing simultaneous optimization of lysis (e.g., 75 °C) and elution (e.g., 65 °C) steps within a single protocol.
Is validation documentation provided for regulatory submissions?
Allsheng supplies IQ/OQ documentation templates, performance qualification (PQ) test scripts, and a comprehensive 3Q summary report—including precision, accuracy, robustness, and carryover testing data—aligned with ISO 15189 and CLSI EP12-A2 guidelines.