Allsheng Auto-Pure 96 Automated Nucleic Acid Extraction System
| Brand | Allsheng |
|---|---|
| Origin | Zhejiang, China |
| Model | Auto-Pure 96 |
| Instrument Type | Magnetic Bead-Based Extraction |
| Automation Level | Dedicated Fully Automatic |
| Sample Throughput per Run | 1–96 (also supports 1–24 configuration) |
| Sample Volume Range | 50–1000 µL and 50–10000 µL |
| Processing Time per Batch | 30–60 minutes |
| Heating Capability | 2 of 8 plate positions with precision temperature control (RT to 120 °C) |
| Display | 7-inch color touchscreen interface |
| Onboard Data Storage | Internal SD card |
| Connectivity | USB, Ethernet, optional Wi-Fi |
| UV Sterilization | Integrated |
| Cross-Contamination Control | Anti-drip baffle, disposable consumables, automated magnetic rod sleeve loading/unloading, barcode recognition |
| Reproducibility | CV < 5% across wells |
| Sensitivity | >95% positive detection rate for samples containing ≥100 copies |
| Power Input | ≤300 W |
| Dimensions (W×D×H) | 560 × 620 × 500 mm |
| Weight | 54 kg |
Overview
The Allsheng Auto-Pure 96 Automated Nucleic Acid Extraction System is an engineered solution for high-throughput, reproducible isolation of genomic DNA and total RNA from diverse biological matrices—including whole blood, serum, plasma, saliva, buccal swabs, tissue homogenates, and cultured cells. It operates on the principle of magnetic bead-based solid-phase extraction, leveraging controlled magnetic field gradients to capture, wash, and elute nucleic acids with minimal manual intervention. Designed for laboratories requiring regulatory-compliant workflows—such as clinical diagnostic labs, reference testing facilities, contract research organizations (CROs), and public health surveillance units—the system delivers consistent yield and purity while supporting traceability, audit readiness, and integration into larger laboratory automation ecosystems.
Key Features
- True high-throughput capacity: Processes 1–96 samples in parallel using standard 96-well deep-well plates (2.2 mL) and compatible 96-position magnetic rod sleeves (1000 µL reaction volume).
- Precision thermal control: Two independently heated plate positions support lysis (RT–120 °C) and elution (RT–120 °C) steps with ±0.5 °C stability, critical for optimizing binding efficiency and minimizing degradation.
- Patented magnetic rod sleeve handling mechanism: Fully automated, contactless loading and unloading ensures >99.99% operational success rate and eliminates operator-induced mechanical stress or misalignment.
- Barcode-driven workflow initiation: Integrated scanner recognizes proprietary reagent kits, auto-loading validated protocols without manual programming—reducing setup time and human error.
- Integrated contamination mitigation: Dual UV-C irradiation (254 nm) during idle cycles, anti-drip baffles, software-controlled magnetic bead drying sequences, and disposable consumables collectively meet ISO 15189 and CLIA requirements for cross-contamination prevention.
- Robust positional accuracy: Eight-plate carousel with real-time position verification—even after external disturbance—ensures reliable reagent access and protocol execution fidelity.
- Comprehensive onboard data management: Generates timestamped, user-attributed extraction reports stored locally on internal SD card; supports export via USB for LIMS integration.
Sample Compatibility & Compliance
The Auto-Pure 96 accommodates a broad spectrum of input materials without hardware modification, including viscous lysates, high-protein content samples, and low-biomass specimens. Its open architecture permits use with third-party magnetic bead kits meeting ISO 13485-manufactured quality standards. The system complies with IEC 61010-1 for laboratory equipment safety and incorporates design elements aligned with FDA 21 CFR Part 11 for electronic records and signatures when paired with compliant software configurations. Built-in audit trail functionality logs all user actions, parameter changes, and run outcomes—enabling GLP/GMP-aligned validation and retrospective review.
Software & Data Management
The embedded Linux-based operating system features a responsive 7-inch capacitive touchscreen with three programmable shortcut keys and optional mouse support. Users can create, edit, duplicate, and archive up to 100 custom protocols, each defining precise timing, temperature ramps, mixing intensity/duration, and magnetic actuation profiles. All runs generate structured JSON-formatted reports containing sample IDs, timestamps, instrument status flags, and pass/fail indicators per step. Data export options include USB mass storage mode and network transfer via Ethernet or optional Wi-Fi module—facilitating remote monitoring, centralized QC dashboards, and seamless interfacing with LIS or ELN platforms.
Applications
- Clinical molecular diagnostics: Pre-analytical processing for PCR, qPCR, digital PCR, and NGS library preparation in infectious disease screening (e.g., SARS-CoV-2, HIV, HBV), oncology liquid biopsy, and pharmacogenomic testing.
- Public health laboratories: High-volume nucleic acid isolation for outbreak response, foodborne pathogen surveillance, and environmental monitoring programs.
- Biobanking and cohort studies: Standardized extraction across thousands of longitudinal samples, ensuring inter-run comparability essential for biomarker discovery.
- Research core facilities: Scalable front-end automation for multi-omics pipelines, particularly where consistency across large-scale functional genomics experiments is required.
- Point-of-need deployment: Compact footprint (0.35 m²) and stable rotary platform enable operation in mobile labs or space-constrained BSL-2 environments.
FAQ
Does the Auto-Pure 96 support both DNA and RNA extraction in the same run?
Yes—provided the selected magnetic bead kit and protocol are validated for dual-modality purification. The system’s independent thermal zones allow simultaneous lysis at elevated temperatures for DNA and milder conditions for RNA integrity preservation.
Can it integrate with robotic liquid handlers or track-and-trace systems?
Yes—via RS-232, Modbus TCP, or custom API (available under OEM agreement), enabling synchronization with robotic arms, tube sorters, and middleware such as Hamilton VENUS or Tecan FluentControl.
Is UV sterilization validated per ISO 15883 or similar standards?
UV-C dose delivery has been verified per ISO 15883-1 Annex C methodology for surface decontamination of internal chamber surfaces; full-cycle validation must be performed onsite using biological indicators per lab SOP.
What level of maintenance is required for long-term reliability?
Scheduled maintenance includes quarterly optical sensor calibration, biannual lubrication of the magnetic drive train, and annual verification of thermal uniformity across heated zones—documented in the included service logbook.
Are consumables supplied with CE-IVD or FDA-cleared claims?
Allsheng-branded reagent kits carry CE-IVD marking; FDA 510(k) clearance applies only to specific kit configurations listed in K-number documentation—users must verify regulatory status for their intended use case.
