Allsheng Auto-Pure Mini Automated Nucleic Acid Extraction System

Overview

The Allsheng Auto-Pure Mini Automated Nucleic Acid Extraction System is an engineered solution for high-fidelity, benchtop-scale nucleic acid isolation in resource-constrained or mobility-critical environments—including field laboratories, mobile diagnostic units, point-of-care testing sites, and academic core facilities requiring space-efficient automation. Unlike conventional magnetic bead-based extractors relying on plate-shaking or lateral motion, the Auto-Pure Mini employs a precision-machined magnetic rod assembly driven by linear screw actuators, enabling vertical, contactless bead capture, transfer, and elution across up to 16 individual wells in parallel. This architecture eliminates cross-contamination risks associated with tip-based liquid handling while maintaining robust performance across variable sample volumes (20–1000 µL) and diverse biological matrices—whole blood, saliva, swabs, tissue homogenates, and cultured cells. Its compact footprint (20 × 26 × 30 cm) reflects deliberate mechanical integration—not functional compromise—retaining full thermal control (ambient +5°C to 120°C), programmable mixing dynamics, and UV sterilization between runs.

Key Features

• Compact form factor optimized for limited bench space or transportability—meets IEC 61010-1 safety standards for portable lab instrumentation.
• Magnetic rod-based separation mechanism ensures consistent bead recovery (>95%) and low inter-well variability (CV ≤3%), critical for downstream qPCR and NGS library preparation.
• Programmable temperature control for both lysis and elution steps, supporting thermally demanding protocols including FFPE-derived DNA extraction and RNA stabilization workflows.
• Multi-mode mixing engine: users define custom oscillation amplitude, frequency, and duration for each step—enabling empirical optimization of binding/wash efficiency with proprietary or third-party magnetic bead kits.
• Integrated UV-C lamp (254 nm) and forced-air exhaust system provide automated decontamination of the processing chamber post-run, reducing carryover risk without manual intervention.
• DC 24 V power architecture enables operation from external lithium battery packs (e.g., 44800 mAh option), extending usability in off-grid or emergency response deployments.
• Low-power display management: touchscreen auto-suspends after user-defined idle time; system remains fully operational and remotely controllable via network interface.

Sample Compatibility & Compliance

The Auto-Pure Mini accommodates standard consumables—including 96-well deep-well plates, single-tube reagent cartridges (AS-17171-01/02), and proprietary magnetic rod sleeves (AS-17031-02)—ensuring compatibility with widely adopted commercial magnetic bead chemistries (e.g., QIAGEN MagAttract, Thermo Fisher KingFisher Flex-compatible kits). It supports ISO/IEC 17025-aligned documentation workflows: onboard audit trail logs timestamp all protocol executions, parameter changes, and error events. Optional barcode scanning (AS-17171-03) enables sample traceability integration with LIMS. While not certified under FDA 21 CFR Part 11 out-of-the-box, its software architecture supports electronic signature implementation and ALCOA+ data integrity principles when deployed within validated laboratory environments compliant with CLIA, CAP, or GLP requirements.

Software & Data Management

The embedded firmware features a responsive 4.3-inch graphical interface with tactile shortcut keys and optional mouse support for glove-compatible operation. All protocols are editable via intuitive drag-and-drop logic blocks—defining temperature ramps, mixing profiles, magnet engagement timing, and aspiration/dispense parameters. Remote access is enabled through native Wi-Fi or Ethernet connectivity: users configure, launch, and monitor runs via the Allsheng Mobile App (iOS/Android), which provides real-time status dashboards, exportable CSV run logs, and version-controlled protocol synchronization across multiple instruments. Data exports comply with MIAME-compliant metadata tagging and can be ingested into ELN platforms via RESTful API (available upon request).

Applications

• Routine clinical diagnostics: extraction of viral RNA (e.g., SARS-CoV-2, Influenza A/B) from nasopharyngeal swabs for RT-qPCR analysis.
• Field-deployable genomic surveillance: rapid isolation of bacterial DNA from environmental water samples for metagenomic sequencing.
• Single-sample forensic processing: discrete extraction of STR-targeted DNA from trace evidence using AS-17171-01 single-tube kits—eliminating batch effects in low-template scenarios.
• Academic research: parallel optimization of lysis buffer compositions and wash stringency across 16 conditions in a single run.
• Veterinary point-of-care: extraction of avian influenza virus RNA from cloacal swabs in mobile veterinary clinics.

FAQ

Does the Auto-Pure Mini support third-party magnetic bead kits?
Yes—it is compatible with any magnetic bead chemistry designed for 96-well plate formats and rod-based systems, provided binding/wash/elution volumes fall within the 20–1000 µL operational range.
Can protocols be exported and shared across multiple instruments?
Yes—protocols are saved as encrypted .apx files and can be transferred via USB drive or synchronized over LAN/Wi-Fi using the Allsheng Mobile App.
Is UV sterilization mandatory between runs?
No—UV cycle activation is user-configurable per protocol; it may be disabled for rapid-turnaround applications where contamination risk is mitigated by other means (e.g., dedicated consumables, cleanroom environment).
What is the minimum required sample volume for reliable extraction?
The system achieves reproducible recovery down to 20 µL input volume, validated using human genomic DNA spiked into PBS and quantified by fluorometry (Qubit) and spectrophotometry (NanoDrop).
How is data integrity ensured during remote operation?
All remote commands are authenticated via TLS 1.2 encryption; execution logs include digital signatures, operator ID, timestamp, and hash-verified parameter sets—supporting 21 CFR Part 11 readiness when paired with institutional identity management infrastructure.