Annuo LAS-3622 Multifunctional HPLC Autosampler

Overview

The Annuo LAS-3622 is a high-precision, multifunctional autosampler engineered for integration with high-performance liquid chromatography (HPLC), ultra-high-performance liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and ion chromatography (IC) systems. It operates on a loop-based sampling architecture—utilizing replaceable fixed-volume loops and precision syringe-driven fluid handling—to deliver reproducible, low-volume injections across diverse analytical workflows. Designed for unattended operation in regulated and research environments, the LAS-3622 supports method-driven automation including timed injections, event-triggered sampling (e.g., via TTL or analog signal input), and PC-scheduled batch runs. Its modular mechanical design accommodates variable vial formats, temperature-sensitive samples (with optional Peltier-cooled sample compartment), and multi-solvent cleaning protocols—ensuring carryover-free analysis and long-term operational stability.

Key Features

• 200-position sample capacity with standard 2 mL vials, enabling high-throughput batch processing without manual intervention
• Adjustable injection volume range from 0.1 µL to 2 mL, supported by interchangeable syringes (50–1000 µL) and selectable loop volumes (50, 100, 250, 1000 µL)
• Three injection modes: full-loop quantitative mode (for highest accuracy), partial-loop mode (for flexibility with limited sample volume), and needle-over-loop mode (for volatile or viscous analytes)
• Integrated leak detection system with real-time pressure monitoring and automatic shutdown upon anomaly recognition
• Collision-avoidance mechanism with optical position feedback and motor torque sensing to protect the injection needle during tray navigation
• Empty-vial detection with configurable response: audible/visual alarm or automatic skip to next valid position
• Interlocked door sensor with user-selectable behavior—illumination activation or system pause upon opening—to maintain environmental integrity and operator safety
• Dual-solvent wash station with independent A/B solvent reservoirs and programmable wash sequences (pre-, post-, or both) for optimized decontamination
• Encoder-based motion control ensuring sub-microliter positioning repeatability and smooth, low-vibration carriage movement
• Optional 4–40 °C Peltier-controlled sample cooling module compliant with ICH Q5C stability storage guidelines

Sample Compatibility & Compliance

The LAS-3622 is compatible with industry-standard 2 mL screw-thread or crimp-top vials (e.g., Agilent, Waters, Thermo Fisher formats) and supports custom tray configurations via software-defined coordinate mapping. It meets baseline mechanical and electrical safety requirements per IEC 61010-1 and electromagnetic compatibility (EMC) standards per IEC 61326-1. While not certified for GMP production use out-of-the-box, its architecture supports audit-ready operation when deployed with validated software: raw data files include timestamped injection logs, sequence metadata, and error codes; all user actions (method edits, parameter changes, manual overrides) are recorded with operator ID and time stamp—enabling alignment with FDA 21 CFR Part 11 principles when paired with compliant LIMS or CDS platforms. The system adheres to ISO 9001-aligned manufacturing practices and conforms to ASTM D7097 (standard practice for HPLC autosampler performance verification).

Software & Data Management

Control is executed via Windows-based Annuo AutoSampler Control Software (ASC v3.2+), supporting both standalone operation and seamless integration with third-party chromatography data systems (CDS) including Chromeleon, Empower, OpenLab CDS, and Analyst. Communication interfaces include USB 2.0, RS-232, and optional Ethernet TCP/IP for networked deployment. Sequence methods define injection order, volume, loop selection, wash steps, temperature setpoints, and trigger conditions—all stored in encrypted XML format with version history. Audit trails capture every command execution, including failed attempts and timeout events. Raw injection logs export to CSV or SQLite for external validation or statistical process control (SPC) analysis. No cloud connectivity or remote telemetry is enabled by default—data residency remains fully under user control.

Applications

The LAS-3622 serves routine QC laboratories performing pharmaceutical assay validation (USP , EP 2.2.46), environmental testing (EPA Method 8081B, 8270), food safety screening (AOAC 2012.01), and academic metabolomics profiling. Its low carryover (<0.005% with dual-solvent wash) and sub-1% RSD at 1 µL support trace-level quantitation in LC–MS bioanalysis. The flexible loop/syringe configuration allows method transfer between legacy HPLC and modern UHPLC platforms without hardware modification. In method development labs, the ability to run parallel sequences—e.g., gradient optimization while maintaining internal standard calibration—reduces cycle time without compromising data integrity.

FAQ

Does the LAS-3622 support direct integration with Thermo Fisher Vanquish or Waters Acquity UPLC systems?
Yes—via standard TTL trigger output and analog voltage input; driver packages for Chromeleon and Empower are available upon request.
Can the sample tray temperature be calibrated and verified?
Yes—the optional Peltier module includes NIST-traceable PT100 sensor output; users may log temperature profiles using external DAQ or validate with certified thermocouple probes.
Is firmware upgrade performed locally or over the internet?
All firmware updates are delivered as signed .bin files via USB stick; no internet connection is required during installation.
What maintenance intervals are recommended for the syringe and loop components?
Syringes should be inspected every 5,000 injections; loops require replacement only if physical damage or persistent carryover is observed—typically beyond 20,000 injections under normal use.
How is compliance with 21 CFR Part 11 ensured?
The system itself provides foundational controls (audit trail, electronic signatures via Windows AD authentication, data immutability); full Part 11 compliance requires validation documentation, procedural SOPs, and integration with a qualified CDS or LIMS platform.