AnTing TXL-4.7 Benchtop Cell Washing Centrifuge
| Brand | AnTing |
|---|---|
| Origin | Shanghai, China |
| Model | TXL-4.7 |
| Instrument Type | Benchtop Centrifuge |
| Speed Range | Up to 4700 rpm |
| Max RCF | 2300 × g |
| Rotor Capacity | 12 × 7 mL swing-bucket |
| Timer Range | 0–99 min |
| Power Supply | 220 V, 50 Hz, 400 W |
| Dimensions (L×W×H) | 380 × 350 × 360 mm |
| Weight | 20 kg |
| Construction | Reinforced polymer housing with electronic safety interlock door |
Overview
The AnTing TXL-4.7 is a benchtop swing-bucket centrifuge engineered specifically for gentle yet effective cell and bacterial pellet washing in routine laboratory workflows. Designed around the principles of sedimentation equilibrium and controlled deceleration, it delivers reproducible separation performance at moderate rotational speeds—optimized to preserve cellular integrity while achieving efficient supernatant removal. Unlike ultra-high-speed or preparative centrifuges, the TXL-4.7 operates within a precisely defined RCF range (up to 2300 × g), making it ideal for post-harvest washing steps in mammalian cell culture, peripheral blood mononuclear cell (PBMC) isolation, bacterial pelleting prior to lysis, and re-suspension protocols requiring minimal mechanical stress. Its compact footprint and low-power consumption (400 W) support integration into biosafety cabinets, clean benches, and shared core facility spaces where space and energy efficiency are prioritized.
Key Features
- Swing-bucket rotor configuration enabling uniform pellet formation across all 12 positions—critical for consistent downstream processing and minimizing sample-to-sample variability.
- Electronic safety interlock door system compliant with IEC 61010-1:2010 requirements for laboratory electrical equipment, preventing accidental operation during lid opening and ensuring operator protection.
- Reinforced polymer chassis providing structural rigidity while reducing overall mass (20 kg), facilitating mobility without compromising vibration damping or operational stability.
- Digital timer with 1-minute resolution (0–99 min) supporting both timed runs and continuous operation modes—enabling protocol standardization across shifts and users.
- Brushless DC motor delivering high torque at low noise levels (<65 dB(A) at maximum speed), suitable for environments requiring acoustic discretion such as teaching labs and quality control suites.
- Thermal management system designed to maintain ambient rotor temperature during extended runs, mitigating heat-induced protein denaturation or cell membrane destabilization.
Sample Compatibility & Compliance
The TXL-4.7 accommodates standard conical-bottom 7 mL polypropylene tubes (e.g., Falcon 352070 or equivalent), supporting common biological matrices including whole blood, cell suspensions (HEK293, CHO, Jurkat), bacterial cultures (E. coli, B. subtilis), and yeast pellets. It meets general-purpose centrifugation requirements outlined in ISO 15190:2020 (Laboratory medicine — Requirements for safety in medical laboratories) and aligns with GLP-relevant operational controls when used in non-regulated research settings. While not certified for clinical diagnostics under CLIA or FDA 21 CFR Part 820, its mechanical design and documentation traceability support internal validation per SOP-driven quality systems.
Software & Data Management
The TXL-4.7 operates via standalone hardware controls with no embedded software or network interface. All operational parameters—including speed, time, and run status—are displayed on an LED panel with tactile button inputs. For laboratories implementing electronic recordkeeping, manual entry of run logs into LIMS or ELN platforms is supported through standardized metadata fields (e.g., instrument ID, date/time stamp, rotor lot number, user ID). Audit trails can be maintained externally in accordance with ALCOA+ principles when paired with documented calibration records and preventive maintenance logs.
Applications
- Cell washing cycles in flow cytometry sample preparation, including red blood cell lysis and antibody labeling workflows.
- Removal of residual culture media or antibiotics from adherent and suspension cell lines prior to RNA extraction or functional assays.
- Bacterial pellet concentration and buffer exchange in plasmid prep and recombinant protein expression pipelines.
- Preparatory centrifugation for microfiltration or size-exclusion chromatography sample loading.
- Standardized sedimentation of PBMCs following Ficoll density gradient separation.
- Teaching applications in undergraduate biochemistry and molecular biology laboratories focusing on centrifugal force fundamentals and rotor dynamics.
FAQ
What tube types are compatible with the TXL-4.7 swing-bucket rotor?
Standard 7 mL conical-bottom centrifuge tubes made from polypropylene or polycarbonate, with maximum height ≤110 mm and diameter ≤14 mm, are fully compatible.
Is rotor balancing required before each run?
Yes—symmetrical loading of opposing buckets is mandatory to prevent vibration-induced wear and ensure bearing longevity. The rotor accepts only even-numbered tube configurations (2–12).
Does the TXL-4.7 support refrigerated operation?
No—this is a non-refrigerated unit. Ambient temperature control of the lab environment is recommended for thermosensitive samples.
Can the timer be bypassed for continuous operation?
Yes—the timer can be set to “∞” mode (via hold function) to enable indefinite spinning until manually stopped.
What maintenance intervals are recommended?
Visual inspection and cleaning every 3 months; full rotor inspection and lubrication every 12 months or after 500 runs—whichever occurs first.
