Anxuyuan AxiLona AXP-100 Nanopore Gene Sequencer

Overview

The Anxuyuan AxiLona AXP-100 is a benchtop third-generation nanopore gene sequencer engineered for high-fidelity, real-time, long-read DNA sequencing. It leverages solid-state CMOS-integrated nanopore sensing architecture—embedding one million parallel nanopore channels on a single custom CMOS chip—to detect ionic current modulations as single-stranded DNA molecules translocate under electrophoretic drive. Unlike optical-based platforms, the AXP-100 eliminates reliance on fluorescent labeling, enzymatic synthesis cycles, or clonal amplification, enabling direct, label-free nucleotide discrimination. Its core biochemistry employs circular consensus sequencing (CCS) via looped template constructs, where each molecule is repeatedly sequenced in a processive manner to generate highly accurate consensus reads. This architecture supports de novo assembly, structural variant detection, epigenetic base modification calling (e.g., 5mC, 6mA), and full-length transcript isoform resolution—critical for functional genomics, microbiome profiling, and complex disease research.

Key Features

• CMOS-based nanopore array: 1 million integrated nanopore sensors per chip, enabling massively parallel signal acquisition with low electronic noise floor and high temporal resolution (≥10 kHz sampling).
• Circular Consensus Sequencing (CCS) workflow: Delivers raw read accuracy of 99.2% and >80% of bases meeting Q20 quality threshold without post-hoc polishing.
• Benchtop footprint: Compact design (0.013 m³ volume, 6.85 kg mass) suitable for shared core facilities, clinical research labs, and field-deployable genomics units.
• Real-time data streaming: Onboard FPGA-accelerated basecalling outputs >1 million reads within the first hour; full run completion in <4 hours depending on library complexity and target coverage.
• No batch dependency: Single-sample operation mode eliminates sample pooling requirements—enabling true “sample-in, answer-out” flexibility for time-sensitive applications.
• Low-input compatibility: Optimized library prep protocol accepts ≥100 ng of genomic DNA, supporting degraded or low-yield samples including FFPE-derived material and metagenomic extracts.

Sample Compatibility & Compliance

The AXP-100 accepts standard Oxford Nanopore-compatible library preparations (e.g., LSK114, SQK-LSK114) and supports double-stranded DNA, native RNA, and hybrid capture-enriched targets. It complies with ISO/IEC 17025:2017 general requirements for competence of testing and calibration laboratories. While not classified as a medical device under FDA 21 CFR Part 809 or EU IVDR, its output data meets analytical validity benchmarks referenced in CLIA-waived assay development frameworks. Sequence data integrity is preserved through hardware-level timestamping, cryptographic hash logging of raw signal files (.fast5), and optional audit-trail-enabled firmware for GLP/GMP-aligned environments.

Software & Data Management

The instrument ships with AxiLona Control Suite v3.x—a Linux-based, containerized software stack supporting local basecalling (via GPU-accelerated Dorado 0.8+ models), alignment (minimap2), variant calling (clair3), and QC reporting (pycoQC, NanoPlot). All software components are open-source compatible and support integration into Galaxy, Nextflow, or Snakemake pipelines. Raw signal files adhere to the community-standard FAST5 format and are automatically archived with SHA-256 checksums. Optional cloud synchronization (AWS S3 or on-premise object storage) enables centralized data governance, version-controlled analysis reproducibility, and role-based access control compliant with HIPAA and GDPR data handling protocols.

Applications

• De novo genome assembly: Resolves repetitive regions, segmental duplications, and centromeric/telomeric structures inaccessible to short-read technologies.
• Full-length transcriptomics: Captures complete splice isoforms, fusion transcripts, and polyadenylation site heterogeneity without fragmentation bias.
• Metagenomic strain tracking: Enables species- and strain-level identification in complex microbial communities using ultra-long haplotype signatures.
• Epigenetic mapping: Detects base modifications natively during sequencing—supporting simultaneous detection of 5-methylcytosine, N6-methyladenine, and hydroxymethylcytosine at single-molecule resolution.
• Rapid pathogen characterization: Facilitates outbreak response via real-time phylogenetic inference from portable sequencing data streams.

FAQ

What sample preparation kits are validated for use with the AXP-100?
Anxuyuan provides protocol compatibility matrices for ONT LSK114, SQK-LSK114, and SQK-RBK114 kits. Custom adapter ligation and PCR-free workflows are supported.
Is the AXP-100 compatible with existing nanopore bioinformatics pipelines?
Yes—the system outputs standard FAST5 and FASTQ files fully interoperable with minimap2, medaka, flye, and other widely adopted tools in the Nanopore Community Ecosystem.
Does the instrument support remote monitoring and control?
Yes—via secure SSH/TLS-enabled web interface and RESTful API endpoints for instrument status, run scheduling, and real-time signal visualization.
How is data integrity ensured during long-duration runs?
Hardware-level cyclic redundancy checks (CRC-32C) are applied to all raw signal packets; corrupted segments are flagged and excluded from basecalling without interrupting acquisition.
Can the AXP-100 be deployed in regulated environments requiring audit trails?
Optional firmware upgrade (AXP-100-ATL v2.1+) enables immutable operator logs, electronic signatures, and 21 CFR Part 11–compliant audit trail export in CSV/JSON formats.