ANYAN AYAN-25K High-Throughput Vacuum Parallel Concentrator with 25-Position Water Bath Heating

Overview

The ANYAN AYAN-25K High-Throughput Vacuum Parallel Concentrator is an engineered solution for rapid, reproducible solvent removal from multiple biological and chemical samples under controlled low-pressure, heated, and oscillated conditions. It operates on the principle of vacuum-assisted evaporation combined with centrifugal force and precise water-bath thermal regulation—enabling simultaneous concentration of up to 25 samples without cross-contamination or thermal gradient-induced variability. Unlike rotary evaporators or nitrogen blow-down systems, this platform integrates three orthogonal physical drivers: (1) reduced system pressure (typically 5–50 mbar, adjustable via digital vacuum control), (2) uniform conductive heating via a deep-well stainless steel water bath (ambient to 100 °C), and (3) programmable horizontal orbital shaking (0–300 rpm) to enhance surface renewal and prevent bumping or foaming. The design ensures that volatile and high-boiling-point solvents—including acetone, methanol, ethyl acetate, and dichloromethane (up to ~90 °C boiling point under vacuum)—are efficiently condensed and recovered in a closed-loop configuration, minimizing environmental release and maximizing analytical recovery.

Key Features

• 25-position parallel processing capacity with individual sealed sample channels—each vial isolated by dedicated O-ring seals and independent exhaust paths to eliminate carryover and backflow during vacuum cycling.
• Dual-tower condensation system coupled with a recirculating chiller unit achieves >95% solvent recovery efficiency for solvents with boiling points ≤90 °C at operating vacuum levels.
• Precision water-bath heating system with ±1 °C temperature stability across all 25 positions—verified by calibrated PT100 sensors embedded in the bath and monitored in real time.
• Programmable horizontal orbital oscillation (0–300 rpm, 8-step resolution) synchronized with vacuum and temperature profiles to optimize mass transfer while preventing emulsion formation or sample splashing.
• 7-inch capacitive touch interface with intuitive icon-driven workflow navigation, password-protected parameter locking, and real-time graphical display of vacuum pressure, bath temperature, and timer status.
• Full-system construction using FDA-compliant, low-outgassing materials: PTFE-coated gas pathways, borosilicate glass condensers, and 316 stainless steel bath housing—ensuring trace-metal-free operation suitable for trace-level analysis (e.g., LC-MS, ICP-MS sample prep).

Sample Compatibility & Compliance

The AYAN-25K accommodates standard 150–200 mL round-bottom or conical collection vials (e.g., Kimble Kontes, Wheaton) and supports a broad range of matrices—including aqueous extracts, organic solvent eluates from SPE cartridges, enzymatic digests, environmental water extracts, and botanical infusions. Its sealed architecture meets ISO 17025 requirements for method validation when used in accredited laboratories performing residue analysis (e.g., pesticide screening per EN 15662), pharmaceutical impurity profiling, or food contaminant quantification. The integrated vacuum control system allows compliance with GLP/GMP documentation standards when paired with optional audit-trail-enabled software (see Software & Data Management). All wetted components conform to USP Class VI biocompatibility testing, and the system is compatible with workflows aligned with ASTM D7066 (solvent recovery efficiency testing) and EPA Method 505/525 series (extraction and concentration of priority pollutants).

Software & Data Management

While the base AYAN-25K operates via standalone touchscreen control, it supports optional PC-based software (ANYAN Concentration Suite v3.2) for full method archiving, electronic lab notebook (ELN) integration, and 21 CFR Part 11–compliant data governance. The software enables creation of SOP-mapped protocols—including multi-stage vacuum ramps, temperature gradients, and oscillation profiles—with automatic timestamped logging of all critical parameters (pressure, temperature, rpm, elapsed time). Audit trails record user login/logout events, parameter modifications, and run completions—exportable as PDF or CSV for regulatory submission. Remote monitoring via Ethernet/Wi-Fi is supported, allowing centralized fleet management in core facilities or QC labs handling high-volume sample batches.

Applications

• High-throughput sample preparation for LC-MS/MS and GC-MS analysis in environmental monitoring (e.g., PAHs, PCBs, phthalates in wastewater).
• Concentration of large-volume herbal extract fractions prior to HPLC fingerprinting or bioactivity screening in natural product discovery.
• Routine residue analysis in food safety labs—concentrating QuEChERS extracts from fruits, vegetables, and animal tissues.
• Pre-concentration of immunoprecipitated protein digests for bottom-up proteomics workflows.
• Stabilization of thermolabile analytes (e.g., prostaglandins, vitamins) via low-temperature vacuum evaporation, avoiding thermal degradation associated with nitrogen blow-down.

FAQ

What vacuum level range does the AYAN-25K support, and how is it regulated?
The system features a digitally controlled diaphragm vacuum pump capable of achieving and maintaining setpoints between 5 mbar and 100 mbar, with real-time feedback via integrated piezoresistive pressure transducers.
Can the instrument handle viscous or foaming samples?
Yes—the programmable oscillation function (0–300 rpm) combined with centrifugal orientation and staged vacuum ramping mitigates foam formation and ensures consistent evaporation kinetics across heterogeneous sample types.
Is the water bath temperature uniform across all 25 positions?
Yes—calibration data confirms ≤±0.5 °C spatial deviation across the entire bath volume at steady state, validated per ISO/IEC 17025 Annex A.3 guidelines for thermal uniformity testing.
Does the system comply with environmental safety standards for solvent emissions?
All solvent vapors are captured and condensed within the dual-tower module; exhaust air is filtered through activated carbon before ambient release, meeting OSHA PEL and EU Directive 2004/42/EC VOC emission thresholds.
What maintenance is required for long-term operational reliability?
Routine tasks include weekly chiller coolant level checks, quarterly condenser cleaning with ethanol, and annual calibration of temperature and pressure sensors using NIST-traceable references.