ANYAN CY-6SPE Fully Automated Solid Phase Extraction System

Overview

The ANYAN CY-6SPE Fully Automated Solid Phase Extraction System is an engineered platform for reproducible, high-throughput sample preparation in trace organic analysis. It operates on the fundamental principle of adsorptive chromatography—where analytes in liquid matrices are selectively retained on solid-phase sorbents (e.g., C18, silica, Florisil, or ion-exchange resins), followed by sequential elution under controlled solvent strength gradients. Unlike vacuum-assisted or gravity-based manual systems, the CY-6SPE employs a positive-pressure manifold architecture with digitally regulated peristaltic and syringe pumps to deliver precise, pulse-free flow across multiple SPE cartridges simultaneously. This design eliminates column channeling, ensures uniform bed compression, and enables robust handling of complex, particulate-laden, or viscous samples—including wastewater, plasma, soil extracts, and fortified food homogenates—without clogging or breakthrough. The system is purpose-built for laboratories requiring method standardization, regulatory compliance, and minimal operator intervention across environmental, pharmaceutical, clinical toxicology, and food safety testing workflows.

Key Features

• Fully automated execution of all SPE steps: conditioning, loading, washing, elution, and optional nitrogen-assisted solvent evaporation.
• Six independent parallel channels with isolated fluid paths—no cross-contamination between samples or solvents.
• Eight dedicated solvent ports supporting gradient elution, multi-step cleanup, and method flexibility without manual solvent switching.
• Positive-pressure delivery using calibrated peristaltic and syringe pumps ensures stable flow rates (0.1–10 mL/min), with linearity coefficient ≥0.999 across the operational range.
• Digital flow rate display and programmable timers enable precise kinetic control—critical for optimizing analyte retention and minimizing matrix co-elution.
• Universal cartridge compatibility: accepts 1 mL, 3 mL, 6 mL, and 12 mL SPE columns from leading manufacturers (e.g., Waters, Agilent, Thermo Fisher, Supelco).
• Chemically resistant fluidic path: pump tubing and column adapters rated for continuous exposure to acetonitrile, methanol, dichloromethane, 10% HCl, 10% NaOH, and sodium hypochlorite.
• Valve-switched manifold architecture minimizes dead volume (<15 µL per port) and eliminates robotic arm wear, enhancing long-term reliability and reducing maintenance intervals.
• Integrated safety protocols: real-time pressure monitoring with automatic shutdown at >1.2 MPa; thermal cutoff at >65°C.

Sample Compatibility & Compliance

The CY-6SPE accommodates diverse biological, environmental, and industrial matrices—including serum, urine, milk, fruit juice, sediment extracts, municipal wastewater, and pesticide-spiked soil leachates. Its ability to process both low-volume (1–10 mL) and high-volume (≥50 mL) samples—via dual-mode loading (pressure-driven for precision, peristaltic for throughput)—supports EPA Method 525.3 (drinking water), ASTM D7271 (petroleum hydrocarbons), and USP <621> chromatographic sample prep requirements. All hardware and software components are designed to align with Good Laboratory Practice (GLP) and current Good Manufacturing Practice (cGMP) documentation standards. Optional audit trail logging, electronic signatures, and user access levels comply with FDA 21 CFR Part 11 when configured with validated software modules.

Software & Data Management

The CY-6SPE is operated via a Windows-based graphical interface featuring drag-and-drop method builder, real-time flow/pressure visualization, and event-triggered notifications. Preconfigured method libraries include EPA 8270D, ISO 17993, and EU SANTE/11312/2021 workflows. Each run generates a timestamped log file containing pump parameters, valve actuation sequence, solvent consumption, and error codes—exportable as CSV or PDF for LIMS integration. Batch scheduling supports unattended overnight operation, with automatic power-down and purge cycles to prevent solvent crystallization or seal degradation.

Applications

• Environmental labs: extraction of PAHs, PCBs, organochlorine pesticides, and PFAS from surface water and groundwater per EPA 1613B and 537.1.
• Pharmaceutical QC: cleanup of API stability samples prior to HPLC-UV or LC-MS/MS analysis, reducing ion suppression and column fouling.
• Clinical toxicology: isolation of opioids, benzodiazepines, and stimulants from whole blood and oral fluid with recovery rates >85% (n=6, RSD <6%).
• Food safety: multi-residue analysis of mycotoxins and veterinary drug residues in cereals and dairy products per EU Commission Regulation (EU) No 2021/808.
• Forensic chemistry: concentration and desalting of seized drug exhibits prior to GC-MS confirmation.

FAQ

What is the maximum recommended sample volume for optimal recovery?
For pressure-driven loading, the upper limit is 50 mL per channel. For larger volumes (e.g., 100–500 mL environmental samples), the integrated peristaltic pump module enables continuous, low-shear loading without column overload.
Can the system perform sequential elution with different solvents on the same cartridge?
Yes—the 8-solvent manifold and programmable valve sequencing allow up to four discrete elution steps per sample, supporting complex cleanup schemes such as mixed-mode SPE.
Is method validation support available?
ANYAN provides application notes, spike-recovery templates, and IQ/OQ documentation packages aligned with ISO/IEC 17025 requirements.
How does the system address carryover concerns in high-sensitivity assays?
Each channel includes a dedicated post-run wash cycle with customizable solvent strength and dwell time; column-to-column carryover is verified at <0.05% using fortified blank injections.
Does the software support remote monitoring and control?
Remote desktop access is enabled via secure LAN/WAN connection; however, critical operations (e.g., method start/stop) require local authentication per cybersecurity best practices.