ANYAN CYFY-8 Precision Multi-Channel Peristaltic Microplate Dispenser

Overview

The ANYAN CYFY-8 Precision Multi-Channel Peristaltic Microplate Dispenser is an engineered solution for high-fidelity, low-volume liquid handling in life science laboratories. Unlike positive-displacement or syringe-based dispensers, the CYFY-8 employs a calibrated peristaltic pumping mechanism—where flexible tubing is compressed sequentially by rotating rollers—to achieve non-contact, contamination-free fluid transfer. This design eliminates carryover risk and enables rapid reconfiguration for diverse reagents without cross-contamination concerns. Optimized for standard SBS-format 96-well microplates, the instrument delivers consistent volumetric performance across all 8 parallel channels, supporting assay workflows in ELISA, cell-based screening, qPCR setup, and high-throughput reagent pre-aliquoting. Its compact footprint (W × D × H: 320 × 300 × 280 mm) and modular architecture make it suitable for benchtop integration in QC labs, core facilities, and regulated R&D environments.

Key Features

• 8-channel peristaltic dispensing system with independent roller-head calibration for inter-channel consistency
• Dispense accuracy ≤1.5% CV at 100 µL (verified per ISO 8655-6 on 96-well plates); minimum dispense volume of 5 µL with stable repeatability
• Dual-speed dispensing mode (high/low) allows optimization for viscous buffers (e.g., glycerol-containing solutions) or volatile solvents
• Intuitive 7-inch capacitive touchscreen interface with graphical workflow builder—supports full-plate, column-wise, or custom-pattern dispensing
• Integrated auto-rinse and aspiration-back function minimizes residual carryover between reagent changes
• Programmable plate-shaking and carriage homing ensure mechanical reproducibility and alignment stability over extended operation cycles
• Removable waste tray with optional external tubing connection enables closed-loop waste management compliant with biosafety Level 2 (BSL-2) containment protocols

Sample Compatibility & Compliance

The CYFY-8 accommodates standard 96-well plates (flat-bottom, U/V-bottom, PCR plates) and accepts custom plate adapters upon request. It handles aqueous buffers, serum-containing media, detergent solutions, and low-viscosity organic mixtures (≤20 cP). Tubing materials (Pharmed® BPT or Norprene®) are certified USP Class VI and FDA-compliant for diagnostic use. While the base unit does not include embedded electronic audit trails, its firmware architecture supports integration with third-party LIMS platforms via RS-232/USB-serial protocol. When operated under documented SOPs—including routine gravimetric calibration using Class I analytical balances (per ASTM E2706)—the system meets functional requirements for GLP and GMP-aligned workflows. Routine maintenance intervals (pump tube replacement every 500–1,000 hours depending on fluid chemistry) align with ISO/IEC 17025 preventive maintenance guidelines.

Software & Data Management

The embedded firmware provides local method storage (up to 50 protocols), real-time dispense log export (CSV format), and timestamped operation records. All parameter changes—including volume setpoints, speed selection, and column mapping—are logged with operator ID fields (when configured with network authentication). Though the device lacks native 21 CFR Part 11 compliance out-of-the-box, its data export structure permits traceable reconciliation with laboratory information management systems (LIMS) or electronic lab notebooks (ELN). Firmware updates are delivered via USB drive with SHA-256 checksum verification to ensure integrity. Calibration logs—including gravimetric validation data against NIST-traceable standards—can be archived externally to fulfill internal QA documentation requirements.

Applications

• Primary and secondary antibody dispensing in ELISA and immunoblotting workflows
• Cell culture medium and supplement addition in 96-well proliferation or cytotoxicity assays
• qRT-PCR master mix distribution prior to thermal cycling
• High-throughput compound dilution series generation for dose-response profiling
• Automated washing steps in bead-based immunoassays (e.g., Luminex xMAP)
• Reagent pre-aliquoting for CRISPR gRNA library screening plates

FAQ

How often should peristaltic tubing be replaced?
Tubing lifetime depends on fluid type and daily usage intensity. For aqueous buffers at moderate flow rates, replacement is recommended every 500 operating hours. For aggressive solvents or high-salinity media, inspect tubing weekly and replace upon visible cracking or loss of elasticity.
Can the CYFY-8 dispense into 384-well plates?
Not natively—the mechanical carriage and nozzle spacing are optimized for 96-well SBS geometry. Custom adapter kits for 384-well formats are available upon engineering consultation, subject to recalibration and precision validation.
Is gravimetric calibration required before each use?
No. Daily verification using a single-point check (e.g., 100 µL into pre-tared tubes) is sufficient for routine operation. Full multi-point calibration per ISO 8655-6 is recommended after tubing replacement, major firmware updates, or quarterly as part of preventive maintenance.
Does the system support remote monitoring or network control?
The CYFY-8 features RS-232 and USB-to-serial interfaces for host PC command control (SCPI-compatible syntax). Ethernet or Wi-Fi connectivity is not built-in but can be enabled via external serial-to-Ethernet converters compatible with Modbus RTU protocol.
What maintenance documentation is provided?
Users receive a comprehensive Maintenance Logbook template, tubing replacement SOPs, calibration worksheets aligned with ASTM E2706, and a traceable certificate of conformance for factory-installed components.