AnYuJie HWS-450 Temperature and Humidity Controlled Incubator
| Brand | AnYuJie |
|---|---|
| Origin | Zhejiang, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | HWS-450 |
| Heating Method | Air-Jacketed Heating |
| Cooling Method | Compressor-Based Refrigeration |
| Humidification Water Tank | External Reservoir |
| Temperature Range (with Humidification) | 5–50 °C |
| Temperature Range (without Humidification) | 5–50 °C |
| Temperature Fluctuation | ±1 °C |
| Temperature Uniformity | ±1 °C |
| Humidity Range | 40–95 %RH |
| Humidity Accuracy | ±5 %RH |
| Chamber Capacity | 450 L |
| External Dimensions (W×D×H) | 640 × 640 × 1850 mm |
Overview
The AnYuJie HWS-450 Temperature and Humidity Controlled Incubator is an engineered environmental simulation system designed for precise, long-term maintenance of biological samples under tightly regulated thermal and hygrometric conditions. It operates on a dual-control principle: air-jacketed heating ensures rapid, uniform temperature stabilization across the chamber, while compressor-based refrigeration enables active cooling down to 5 °C—critical for applications requiring sub-ambient incubation. Humidity control is achieved via ultrasonic humidification coupled with a high-capacity external water reservoir, delivering stable RH output between 40 % and 95 % without internal condensation buildup. The unit’s stainless-steel forced-air circulation ductwork and double-door insulated structure minimize thermal gradients and external environmental interference, supporting reproducible experimental outcomes in regulated life science workflows.
Key Features
- Air-jacketed heating system with independent PID control for fast thermal response and minimal overshoot
- Original Tecumseh (France) hermetic compressor with high/low pressure protection and delayed startup logic to extend service life
- Ultrasonic humidifier integrated with external water tank—eliminates internal water reservoir contamination risks and simplifies maintenance
- Dual-door design with magnetic seal and low-emissivity glass window—enhances thermal insulation and reduces energy consumption
- Stainless-steel interior chamber and reinforced airflow duct—resists corrosion, supports cleaning validation, and ensures uniform temperature distribution (±1 °C)
- Comprehensive safety architecture: over-temperature cutoff, sensor fault detection, door-open alarm, and audible/visual fault indicators
Sample Compatibility & Compliance
The HWS-450 accommodates standard microbiological culture vessels (e.g., Petri dishes, flasks, multi-well plates), plant tissue culture trays, and BOD bottles up to 450 mL volume. Its chamber geometry permits flexible shelving configurations and unobstructed airflow around stacked loads. While not certified to ISO 13485 or FDA 21 CFR Part 11 out-of-the-box, the incubator’s hardware-level safeguards—including non-volatile parameter storage, tamper-resistant calibration locks, and event-logged alarms—support alignment with GLP/GMP documentation requirements. Users may implement supplementary SOPs for humidity validation per ASTM E2749 or ISO 17025-compliant uncertainty budgets.
Software & Data Management
The HWS-450 features a microprocessor-based controller with real-time display of setpoints, actual values, and system status. All operational parameters—including temperature, humidity, runtime, and alarm history—are stored locally for ≥30 days. Optional RS-485 Modbus RTU interface enables integration into centralized laboratory monitoring systems (e.g., LabVantage, Siemens Desigo). Data export is supported via USB flash drive (CSV format), facilitating audit-ready reporting. No cloud connectivity or proprietary software installation is required; configuration remains accessible through intuitive front-panel navigation.
Applications
- Biochemical oxygen demand (BOD) testing per APHA Standard Methods 5210B and ISO 5815-1
- Microbial growth studies under controlled hygrothermal stress (e.g., mold inhibition assays, yeast fermentation kinetics)
- Seed germination and plant tissue culture under defined RH profiles (e.g., drought simulation, callus induction)
- Environmental stability testing of pharmaceutical intermediates per ICH Q1A(R2)
- Quality control of diagnostic reagents and cell-based assay kits requiring ambient-conditioned storage
- Long-term viability assessment of aquatic larvae, insect eggs, and nematode cultures
FAQ
What is the recommended maintenance schedule for the external humidification reservoir?
Refill the external water tank daily when operating above 70 %RH; clean the tank and ultrasonic transducer weekly using deionized water and soft brush to prevent mineral scaling.
Can the HWS-450 maintain 5 °C with 95 %RH simultaneously?
No—condensation risk limits combined low-temperature/high-humidity operation. At 5 °C, maximum sustainable RH is ≤80 % due to dew point constraints.
Is the incubator suitable for use in ISO Class 5 cleanrooms?
Yes—its sealed construction, zero internal gasket adhesives, and stainless-steel surfaces meet particulate shedding requirements; however, HEPA filtration must be provided externally.
How is temperature uniformity verified during qualification?
Per ISO 15265, 9-point mapping (3×3 grid at mid-height) using NIST-traceable thermistors confirms spatial deviation ≤±1 °C at 37 °C/50 %RH steady state.
Does the unit support remote monitoring via Ethernet or Wi-Fi?
No—only RS-485 serial communication is available; Ethernet/Wi-Fi modules are not factory-installed or validated.
