AOE U110Pro Ultraviolet-Visible Spectrophotometer
| Brand | AOE |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Product Origin | Domestic (China) |
| Model | U110Pro |
| Price Range | USD 700–1,400 |
| Optical Design | Single-Beam |
| Detector Type | Silicon Photocell |
| Wavelength Range | 200–1100 nm |
| Wavelength Scanning Mode | Motorized Auto-Scan |
| Spectral Bandwidth | 4.0 nm |
| Wavelength Accuracy | ±1.5 nm |
| Stray Light | ≤0.1% T at 220 nm and 340 nm |
| Wavelength Repeatability | ≤0.2 nm |
| Baseline Stability | ±0.001 Abs/h at 500 nm |
Overview
The AOE U110Pro Ultraviolet-Visible Spectrophotometer is a precision single-beam optical instrument engineered for routine quantitative and qualitative analysis in academic laboratories, quality control environments, and industrial R&D settings. It operates on the fundamental principle of Beer-Lambert law-based absorbance measurement, utilizing a high-efficiency holographic grating (1200 lines/mm) to resolve spectral features across a broad 200–1100 nm range—spanning the deep UV, near-UV, visible, and near-infrared regions. Its fixed 4.0 nm spectral bandwidth ensures consistent resolution for standard applications such as concentration determination, kinetic assays, and spectral scanning of organic dyes, nucleic acids, proteins, and transition metal complexes. Designed with mechanical robustness and thermal stability in mind, the U110Pro delivers baseline drift of no more than ±0.001 Abs/h at 500 nm—enabling reliable long-duration measurements without frequent recalibration.
Key Features
- Single-beam optical architecture with motorized wavelength drive for fully automated spectral scanning and peak search
- Holographic diffraction grating (1200 grooves/mm) optimized for high light throughput and low polarization dependence
- Silicon photocell detector with linear response over 0–3.0 Abs and integrated temperature compensation circuitry
- Digital wavelength positioning system ensuring repeatability within ±0.2 nm
- Onboard microprocessor control with embedded firmware supporting absorbance, transmittance, and concentration modes
- Front-panel LCD interface with intuitive menu navigation and real-time spectrum preview during scanning
- USB 2.0 interface compliant with Windows-based PC connectivity for data export and remote operation
Sample Compatibility & Compliance
The U110Pro accommodates standard 10 mm pathlength quartz or glass cuvettes (including matched pairs for reference/blank subtraction), as well as optional micro-volume adapters (e.g., 50–500 µL capillary cells). It supports common solvent systems including water, ethanol, methanol, and buffered aqueous solutions. While not certified for GMP-regulated production environments, the instrument meets general laboratory requirements aligned with ISO/IEC 17025 calibration traceability frameworks when used with NIST-traceable standards. Its optical performance specifications—including stray light ≤0.1% T at critical wavelengths (220 nm and 340 nm) and wavelength accuracy of ±1.5 nm—conform to the minimum criteria outlined in ASTM E275 and ISO 6223 for routine UV-Vis instrumentation validation.
Software & Data Management
The U110Pro is compatible with AOE’s proprietary SpectraView Pro software (v3.2+), a Windows-based application enabling spectral acquisition, multi-curve overlay, derivative spectroscopy, and time-scan kinetics. Raw absorbance data are exported in CSV and ASCII formats for integration into third-party platforms such as MATLAB, OriginLab, or Excel-based QA/QC workflows. The software supports user-defined method templates, automatic baseline correction algorithms, and audit-ready report generation—including operator ID, timestamp, instrument serial number, and calibration history. While the U110Pro does not implement full 21 CFR Part 11 electronic signature functionality, its data logging structure supports GLP-aligned documentation practices when paired with controlled access protocols and manual sign-off procedures.
Applications
- Quantitative analysis of pharmaceutical actives (e.g., paracetamol, aspirin) per USP guidelines
- Determination of protein concentration via Bradford or BCA assay at 595 nm or 562 nm
- Monitoring enzymatic reaction kinetics (e.g., NADH oxidation at 340 nm)
- Verification of dye purity and λmax in textile and pigment manufacturing
- Educational use in undergraduate chemistry labs for Beer’s law verification and equilibrium constant studies
- Water quality testing—including nitrate (220 nm), phosphate (880 nm via molybdenum blue), and COD estimation
FAQ
Is the U110Pro suitable for regulated pharmaceutical QC environments?
It is intended for non-GMP research, teaching, and general QC use. For FDA-submitted batch release testing, a dual-beam spectrophotometer with full 21 CFR Part 11 compliance and IQ/OQ/PQ documentation support is recommended.
Can I use quartz cuvettes below 200 nm?
No—the instrument’s lower limit is 200 nm due to atmospheric oxygen absorption and optical window transmission constraints; quartz cuvettes are required only for measurements below ~350 nm, but the U110Pro’s UV cutoff is defined by its grating and detector response, not cuvette material alone.
Does the U110Pro support kinetic time-scan mode?
Yes—when connected to SpectraView Pro software, it enables time-resolved acquisition at user-defined intervals (100 ms to 60 s per point) with up to 10,000 data points per run.
What calibration standards are recommended for routine verification?
NIST-traceable holmium oxide (Ho₂O₃) and didymium filters for wavelength verification; potassium dichromate in 0.005 M H₂SO₄ for photometric accuracy checks at 235, 257, 313, and 350 nm.
Is external power supply or voltage stabilization required?
A stable 100–240 VAC, 50/60 Hz input is sufficient; no external UPS or line conditioner is mandated under typical lab conditions, though surge protection is advised.
