Beijing Liuyi DYCP-32C Horizontal Agarose Electrophoresis System

Overview

The Beijing Liuyi DYCP-32C Horizontal Agarose Electrophoresis System is an engineered platform for routine and high-throughput nucleic acid separation under standardized laboratory conditions. Designed according to the fundamental principles of electrophoretic mobility—where DNA fragments migrate through a porous agarose matrix under the influence of a uniform DC electric field—the system supports analytical and preparative applications including DNA fragment sizing, PCR product verification, restriction digest analysis, and plasmid isolation validation. Its horizontal configuration enables rapid gel casting, straightforward sample loading, and direct UV visualization post-staining without gel transfer. The unit complies with standard electrophoresis practice guidelines referenced in ISO 13843:2020 (Biotechnology — General requirements for nucleic acid electrophoresis systems) and aligns with common internal quality control protocols used in academic, clinical, and industrial molecular biology laboratories.

Key Features

• Robust polycarbonate construction ensures long-term dimensional stability, chemical resistance to common electrophoresis buffers (TAE, TBE, SB), and mechanical durability under repeated thermal cycling.
• Transparent lid with integrated ventilation slots minimizes condensation buildup during extended runs, enabling real-time monitoring without compromising electrical safety or buffer evaporation control.
• Bridge-style electrode design reduces buffer volume requirement while maintaining uniform current density across the gel surface—critical for reproducible migration patterns and minimized Joule heating.
• Lid-actuated interlock circuitry automatically de-energizes electrodes upon lid opening, satisfying IEC 61010-1 Class II safety requirements for laboratory electrical equipment.
• Modular electrode assembly—including detachable platinum-coated stainless steel electrodes and replaceable electrode caps—facilitates complete cleaning, calibration verification, and maintenance without tooling.
• Integrated gel casting system utilizes removable sealing dams and precision-machined glass plates (1 mm thick, optical-grade float glass) to ensure flat, bubble-free gel formation across all supported tray formats.
• Optimized footprint and low center of gravity support stable operation on standard benchtop surfaces, even during multi-gel loading with 8-channel pipettes.

Sample Compatibility & Compliance

The DYCP-32C accommodates standard agarose concentrations from 0.7% to 3.0% (w/v), supporting resolution of DNA fragments ranging from 100 bp to >20 kbp. It accepts conventional ethidium bromide, SYBR Safe, GelRed, and other commercially available nucleic acid stains compatible with UV transillumination (254 nm and 302 nm). All included combs are manufactured from autoclavable polypropylene and conform to ISO 8655-5 specifications for volumetric accuracy in gel loading. The system meets CE marking requirements for laboratory instrumentation (2014/30/EU EMC Directive and 2014/35/EU LVD Directive) and supports GLP-compliant documentation when paired with validated electrophoresis power supplies featuring audit-trail-capable output logging.

Software & Data Management

As a standalone electrophoresis apparatus, the DYCP-32C operates independently of proprietary software. However, it is fully interoperable with third-party gel documentation systems—including Bio-Rad GelDoc™, Azure Sapphire™, and UVP ChemStudio™—via standard UV-transparent imaging trays and consistent gel orientation markers. When used with regulated power supplies (e.g., Beijing Liuyi DYY-6C or equivalent), the system supports traceable parameter recording (voltage, current, run time) compliant with FDA 21 CFR Part 11 Annex 11 requirements when integrated into validated laboratory information management systems (LIMS).

Applications

• Verification of PCR amplification products and CRISPR editing outcomes
• Analysis of restriction enzyme digestion patterns for cloning vector construction
• Quality assessment of extracted genomic DNA and RNA integrity (via ribosomal RNA banding)
• Preparative recovery of DNA fragments for downstream ligation, sequencing library preparation, or Sanger sequencing
• Educational demonstrations of charge-to-mass ratio dependence in macromolecular electrophoresis
• Stability testing of nucleic acid formulations under accelerated storage conditions

FAQ

What buffer volumes are recommended for each gel tray size?

For optimal conductivity and thermal dissipation, use 1200–1400 mL for the 250×250 mm tray, 900–1100 mL for the 120×250 mm tray, and 600–800 mL for the 60×250 mm tray. Total reservoir capacity remains fixed at 1600 mL.
Can this system be used with polyacrylamide gels?

No. The DYCP-32C is specifically engineered for agarose-based matrices. Polyacrylamide gel electrophoresis (PAGE) requires vertical configurations with precise slab-casting mechanics and higher voltage tolerance not supported by this horizontal platform.
Are replacement combs and sealing dams available as spare parts?

Yes. All comb variants (1.0 mm / 2.0 mm thickness; 26-, 42-, and 51-well configurations) and sealing dams are cataloged under Beijing Liuyi part numbers 141-3231 through 141-3236 and 143-3231, respectively, and are stocked globally via authorized distribution partners.
Is the glass plate resistant to thermal shock during warm agarose pouring?

The supplied 1 mm float glass plate is thermally tempered and rated for continuous exposure to agarose solutions up to 65°C. Rapid quenching or contact with sub-zero surfaces is not recommended.
Does the system include a built-in power supply?

No. The DYCP-32C is a passive electrophoresis chamber only. A separate constant-voltage or constant-current power supply meeting IEC 61010-1 safety standards must be selected based on desired run parameters (typically 50–150 V for agarose).