Beijing Liuyi DYCP-44P Fast Electrophoresis System (Model 122-4470)

Overview

The Beijing Liuyi DYCP-44P Fast Electrophoresis System (Model 122-4470) is a compact, benchtop nucleic acid electrophoresis apparatus engineered for high-throughput DNA and RNA separation under standardized buffer conditions. It operates on the principle of constant-voltage or constant-current electrophoresis in submerged horizontal gel matrices—primarily agarose and native or denaturing polyacrylamide gels—enabling rapid migration of charged biomolecules through porous gel media under applied electric fields. Designed for laboratories requiring consistent, reproducible resolution of PCR amplicons, restriction fragments, and plasmid isoforms, the system supports accelerated run times (typically 20–45 minutes at 100–250 V) without compromising band sharpness or molecular weight discrimination. Its integrated casting tray, adjustable leveling feet, and rigid acrylic chamber ensure uniform gel thickness and minimal buffer leakage—critical factors influencing electrophoretic mobility reproducibility and inter-run comparability.

Key Features

• Compact footprint (210 × 200 × 85 mm) optimized for space-constrained biosafety cabinets and shared core facilities.
• Standard 170 × 140 mm gel platform accommodates up to six parallel sample lanes per comb; compatible with both 1.0 mm (33-tooth) and 1.5 mm (17-tooth) combs for variable loading capacity and resolution requirements.
• Integrated leveling legs ensure precise horizontal alignment during gel pouring—reducing meniscus distortion and improving band straightness across the entire gel surface.
• Acrylic electrophoresis chamber with chemically resistant seals and low-profile lid design minimizes evaporation and maintains stable thermal equilibrium during extended runs.
• Modular component set includes dual sealing bars, removable buffer reservoirs, and standardized electrode terminals compatible with common DC power supplies (0–300 V / 0–1000 mA range).
• Buffer volume of 200 mL provides sufficient ionic strength for consistent conductivity while enabling rapid heat dissipation—reducing band smearing caused by Joule heating.

Sample Compatibility & Compliance

The DYCP-44P supports routine analytical applications involving double-stranded DNA (0.1–10 kb), single-stranded RNA, and small DNA fragments generated via PCR, RFLP, or cloning workflows. It is routinely employed in academic research, clinical molecular diagnostics, and QC testing of nucleic acid reagents. While the instrument itself does not carry CE or FDA 510(k) certification as a standalone medical device, its operational parameters align with widely adopted laboratory standards including ISO/IEC 17025 for testing laboratories and CLSI EP05-A3 guidelines for method validation in nucleic acid analysis. When used with traceable power supplies and calibrated UV transilluminators, data generated meets GLP documentation requirements for internal assay development and preliminary screening.

Software & Data Management

As a hardware-only electrophoresis chamber, the DYCP-44P does not include embedded firmware or proprietary software. It functions as a passive, analog-compatible module within existing lab infrastructure—fully interoperable with third-party power supplies featuring digital displays, time-programmable ramps, and audit-trail logging (e.g., compatible with systems compliant with FDA 21 CFR Part 11 when paired with validated controllers). Users retain full control over voltage, current, and run duration via external instrumentation, enabling integration into LIMS-driven workflows where experimental metadata (gel dimensions, buffer composition, run time) are manually or automatically recorded alongside image capture from downstream gel documentation systems.

Applications

• Routine verification of PCR product size and specificity prior to sequencing or cloning.
• High-frequency genotyping assays requiring rapid turnaround, such as pathogen detection (e.g., SARS-CoV-2 amplicon screening) and SNP fragment analysis.
• Quality control of plasmid preps, restriction digests, and ligation reactions in synthetic biology pipelines.
• Educational laboratory instruction—its mechanical simplicity, visual clarity, and robust construction support hands-on training in fundamental electrophoretic theory and technique.
• Preparative gel excision for downstream enzymatic processing, where consistent band geometry facilitates clean extraction and minimizes cross-contamination.

FAQ

What gel types are supported by the DYCP-44P chamber?

Agarose gels (0.7–3.0% w/v) and native or SDS-denatured polyacrylamide gels (8–20% T) are fully compatible when prepared within the specified 170 × 140 mm format and 1.0–1.5 mm thickness range.
Is the unit supplied with a power supply?

No—the DYCP-44P is a gel tank only; users must provide an external DC power supply rated for electrophoresis (typically 0–300 V, 0–1000 mA, constant voltage or constant current mode).
Can this system be used for protein electrophoresis?

Yes, for native PAGE or SDS-PAGE of low-molecular-weight proteins (<100 kDa), provided appropriate gel concentration, running buffer (e.g., Tris-Glycine-SDS), and cooling measures are implemented to manage resistive heating.
How is buffer level monitored during operation?

The transparent acrylic chamber allows real-time visual inspection of buffer height; minimum operational level is marked at 150 mL to ensure full submersion of gel and electrodes.
Are replacement parts available for long-term maintenance?

Yes—standardized components including combs (141-4411), sealing bars (414-4702), and electrode leads (144-0011) are stocked by authorized distributors and supplied with batch-specific traceability documentation.