Beijing Liuyi DYCZ-20G DNA Sequencing Electrophoresis Apparatus

Overview

The Beijing Liuyi DYCZ-20G DNA Sequencing Electrophoresis Apparatus is a dual-gel, height-adjustable vertical electrophoresis system engineered for high-resolution nucleic acid separation in Sanger sequencing, SSR (Simple Sequence Repeat) genotyping, RFLP analysis, and other molecular marker applications. It operates on the principle of polyacrylamide gel electrophoresis (PAGE) under denaturing conditions—typically using urea-based gels—to resolve single-stranded DNA fragments differing by as little as one nucleotide. The system’s modular architecture supports two distinct gel heights (130 mm and 190 mm), enabling method flexibility without hardware replacement. Its design emphasizes reproducibility, buffer economy, and operational safety—features critical for laboratories conducting routine, high-throughput genotyping or core facility-based sequencing support.

Key Features

• Height-adjustable main frame accommodating either 130 mm or 190 mm gel assemblies—enabling optimization for resolution (190 mm) or throughput (130 mm).
• Simultaneous dual-gel electrophoresis capability: two independent 190 × 316 mm gels or six 130 × 316 mm gels per casting batch, ensuring inter-gel consistency and reducing run-to-run variability.
• 204-sample capacity per run (2 × 102-well combs, 1.0 mm thickness), compatible with multichannel pipettes for rapid, uniform loading—reducing manual error and improving assay robustness.
• Optimized buffer reservoir design: 600 mL upper chamber + ≥600 mL lower chamber, sufficient for dual-gel operation while minimizing buffer consumption by ~50% versus conventional single-gel systems; eliminates frequent pH recalibration between runs.
• Integrated safety mechanism: automatic power cutoff upon lid opening, compliant with IEC 61010-1 requirements for laboratory electrical equipment.
• Optical-grade PMMA construction provides structural rigidity, chemical resistance to acrylamide and urea solutions, and full visual monitoring of electrophoretic migration and buffer levels.

Sample Compatibility & Compliance

The DYCZ-20G is validated for use with standard denaturing polyacrylamide gels (e.g., 6–8% T, 7 M urea) commonly employed in manual and semi-automated DNA sequencing workflows. It accommodates common labeling chemistries—including radioactive (³²P, ³⁵S) and non-radioactive (fluorescent dyes, silver staining)—and is compatible with downstream visualization methods such as autoradiography, phosphorimaging, and UV transillumination (when used with appropriate imaging systems). While not an FDA-cleared IVD device, the instrument meets general laboratory safety standards referenced in ISO/IEC 17025:2017 for testing laboratories and supports GLP-aligned documentation practices via external logbook integration. Its consistent gel dimensions and buffer dynamics facilitate method transfer across labs adhering to ASTM E2576 (Standard Guide for Electrophoretic Separation of Nucleic Acids).

Software & Data Management

The DYCZ-20G is a standalone electrophoresis apparatus with no embedded microcontroller or proprietary software. All operational parameters—including voltage, current, and run duration—are controlled externally via standard DC power supplies (e.g., 0–600 V, 0–100 mA). This analog architecture ensures long-term compatibility with existing lab infrastructure and avoids vendor lock-in. Users maintain full traceability through manual run logs or integration with LIMS platforms via external data entry. For regulated environments requiring audit trails (e.g., clinical research under 21 CFR Part 11), laboratories may pair the system with validated electronic lab notebooks (ELNs) that record operator ID, gel lot numbers, buffer preparation dates, and run metadata.

Applications

• Sanger DNA sequencing ladder resolution (e.g., dideoxy termination products)
• SSR and microsatellite allele sizing for plant/animal breeding programs
• RFLP and AFLP fragment profiling in population genetics
• Verification of PCR product integrity and primer specificity
• Quality control of synthetic oligonucleotides and gene fragments
• Educational training in molecular biology techniques requiring hands-on gel handling and interpretation

FAQ

Is the DYCZ-20G compatible with automated gel dryers or vacuum blotters?
Yes—the gel dimensions (190 × 316 mm and 130 × 316 mm) conform to standard vertical gel drying frames and blotting modules used in legacy and modern transfer systems.
Can I use pre-cast gels with this apparatus?
No—the DYCZ-20G is designed exclusively for hand-cast polyacrylamide gels using its integrated casting frames; it does not support commercial pre-cast gel formats.
What power supply specifications are recommended?
A programmable DC power supply capable of delivering 0–600 V and 0–100 mA is required; constant-power or constant-current modes are both supported depending on experimental protocol.
Does the system include temperature control?
No—it operates at ambient temperature; users requiring thermal regulation must implement external cooling (e.g., circulating chillers connected to buffer reservoirs) per protocol requirements.
Are replacement parts (combs, spacers, glass plates) available separately?
Yes—Beijing Liuyi supplies OEM-compatible consumables including 102-well 1.0 mm combs, 1.5 mm spacers, and borosilicate glass plates sized for both 130 mm and 190 mm configurations.