Bruker o-Advance Nano/UHPLC System (Refurbished)

Overview

The Bruker o-Advance Nano/UHPLC System is a purpose-engineered nanoflow and capillary-scale liquid chromatography platform designed for high-sensitivity, low-volume LC-MS applications. Operating across a broad pressure range—from conventional HPLC up to 10,000 PSI—this refurbished system delivers precise gradient delivery, minimal system dwell volume, and exceptional flow stability essential for reproducible peptide mapping, proteomics, metabolomics, and trace-level small-molecule quantitation. Its core architecture employs dual-pump solvent delivery with real-time flow feedback sensors on each pump channel, enabling sub-microliter-per-minute control fidelity critical for nanoLC workflows. The system integrates seamlessly with Bruker’s HyStar and Compass CDS software environments via native USB communication modules, supporting method transfer, audit-trail-enabled operation, and GLP-compliant data handling when deployed in regulated laboratories.

Key Features

• Ultra-high-pressure binary pumping system rated to 10,000 PSI, optimized for both capillary (50–300 µm ID) and nanobore (25–75 µm ID) column formats
• Nanoflow/capillary splitless injector with programmable flow range from 1 nL/min to 50 µL/min, eliminating need for post-column splitting
• Real-time flow monitoring sensors on each pump head, delivering <0.25% RSD flow precision at rates up to 8 µL/min
• Integrated membrane-based degassing unit minimizing baseline noise and bubble-induced retention time drift
• Modular USB-controlled architecture enabling plug-and-play integration with mass spectrometers (e.g., timsTOF, maXis, Impact II) and third-party CDS platforms
• Factory-reconditioned and functionally validated per Bruker OEM specifications; includes full calibration report and performance verification documentation

Sample Compatibility & Compliance

The o-Advance supports a wide range of sample matrices—including biological fluids, tissue digests, purified proteins, and synthetic organic compounds—when coupled with appropriate column chemistries (C18, HILIC, SCX, porous graphitic carbon). Its low internal volume (<1.5 µL total dwell volume) ensures rapid gradient equilibration and sharp peak formation, directly enhancing MS ionization efficiency and signal-to-noise ratio. The system meets foundational requirements for ISO/IEC 17025-compliant laboratories and supports 21 CFR Part 11 readiness through HyStar’s electronic signature and audit trail capabilities. All refurbished units undergo rigorous leak testing, pressure cycling validation, and gradient accuracy verification against NIST-traceable standards prior to release.

Software & Data Management

Control and data acquisition are fully supported through Bruker’s HyStar (v4.2 or later) and Compass CDS software suites. These platforms provide method editor tools for multi-step gradient programming, automatic valve sequencing, and synchronized MS trigger events. Raw data files (.yep, .d) are stored in vendor-native formats compatible with open-source processing pipelines (e.g., MaxQuant, Skyline, OpenMS). Instrument logs—including pressure traces, flow deviations, error codes, and user login timestamps—are retained with immutable timestamps, fulfilling GLP/GMP documentation expectations. Remote diagnostics and firmware updates are accessible via secure SSH connections when deployed behind institutional firewalls.

Applications

• Bottom-up and top-down proteomics workflows requiring high-resolution peptide separation prior to timsTOF or Q-TOF analysis
• Pharmacokinetic studies involving low-abundance drug metabolites in plasma or urine extracts
• Single-cell metabolomics using capillary columns with sub-nanoliter injection volumes
• Quality control of monoclonal antibody charge variants using weak cation exchange (WCX) nanoLC
• Method development for regulatory submissions where system suitability testing must demonstrate repeatability (RSD <1.0% for RT, <2.0% for peak area)

FAQ

Is this instrument fully refurbished and tested before shipment?
Yes. Each o-Advance system undergoes comprehensive functional validation—including pressure hold tests, gradient linearity assessment, flow accuracy verification, and detector interface handshake protocols—per Bruker’s original service manuals.
What documentation accompanies the system?
You receive a Certificate of Reconditioning, full calibration report, performance verification log, and OEM-compatible software license keys for HyStar or Compass CDS.
Can this system be integrated with non-Bruker mass spectrometers?
Yes. The USB-based control interface supports TTL-triggered acquisition and analog/digital I/O synchronization with Thermo, Sciex, Waters, and Agilent MS platforms via standard LabVIEW or Python API wrappers.
Does the 6-month warranty cover parts and labor globally?
Warranty covers hardware defects and labor for onsite or depot repair within North America, Europe, and APAC regions; extended service plans are available upon request.
Are consumables and spare parts still available for the o-Advance platform?
All critical wear components—including pump seals, check valves, injector rotors, and degasser membranes—are actively stocked by Bruker and authorized distributors through 2027.