CHEETAH MP100 Medium-Pressure Preparative Liquid Chromatography System
| Origin | Tianjin, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (PRC) |
| Model | CHEETAH MP100 |
| Price Range | USD 14,000–28,000 |
| Application Level | Laboratory-Scale |
| Instrument Type | Medium-Low Pressure Preparative HPLC |
| Flow Rate Range | 1–100 mL/min |
| Flow Accuracy | ±2% |
| Flow Precision (RSD) | ±2% |
| Maximum Operating Pressure | 200 psi |
| Wavelength Range | 200–400 nm (standard detector) |
| Wavelength Accuracy | ±1 nm |
| Baseline Noise | 3 × 10⁻⁴ AU |
| Data Acquisition Frequency | 3 Hz |
Overview
The CHEETAH MP100 Medium-Pressure Preparative Liquid Chromatography System is an integrated, benchtop-scale purification platform engineered for reproducible, scalable isolation of organic, natural, and pharmaceutical compounds under medium-pressure conditions (up to 200 psi). Based on high-efficiency liquid chromatography principles—specifically gradient-elution flash chromatography—the system delivers robust separation performance across a broad range of compound polarities and molecular weights. It operates within the preparative LC regime, bridging the gap between analytical HPLC and high-pressure preparative systems, making it especially suitable for milligram-to-gram scale purification in discovery-phase chemistry, natural product isolation, synthetic intermediate refinement, and early-stage process development. Its modular architecture supports seamless integration with complementary detectors—including refractive index (RI) and evaporative light scattering (ELSD)—enabling universal detection of UV-transparent analytes such as carbohydrates, lipids, and non-chromophoric polymers.
Key Features
- 12.1-inch industrial-grade capacitive touchscreen interface with intuitive graphical workflow navigation—no external PC required for basic operation.
- Real-time “peak-to-tube” mapping: chromatographic peaks are dynamically linked to physical collection positions during run execution, enabling immediate manual intervention or conditional fraction triggering.
- Chemically resistant stainless-steel and PTFE-coated work surface rated for exposure to chlorinated solvents, acetonitrile, methanol, ethyl acetate, and other common eluents used in flash and medium-pressure chromatography.
- Tool-free, modular column holder design accommodating standard Flash columns (15–80 mm i.d.) and custom glass columns (up to 50 mm i.d.), with optional high-capacity auto-sampler supporting both solid and liquid sample introduction.
- Dual-wavelength UV/Vis detector featuring independent optical paths and dual-channel signal processing—enabling simultaneous monitoring at two user-defined wavelengths (e.g., 220 nm for amide bond detection and 254 nm for aromatic tracking), critical for co-eluting species with disparate absorption maxima.
- Binary or quaternary solvent selection capability via precision stepper-motor-driven proportioning valves; gradient programming supports up to 20 segments with real-time editing during active runs.
- Integrated 2D fraction collector with programmable X-Y positioning, compatible with standard test tube racks (13 mm, 15 mm, 18 mm, 25 mm, and 100 mL vials), plus user-defined container profiles for large-volume (up to 1 L) collection vessels.
Sample Compatibility & Compliance
The CHEETAH MP100 is validated for use with diverse sample matrices including crude plant extracts, reaction mixtures from cross-coupling or asymmetric synthesis, fermentation broths, and enzymatic digests. Its solvent compatibility extends to aggressive mobile phases (e.g., DCM/methanol gradients, THF/water systems) without compromising seal integrity or pump longevity. The system meets fundamental requirements for GLP-compliant laboratory environments: pressure and leak sensors provide continuous hardware-level safety monitoring, while all method parameters—including flow rate, gradient profile, collection logic, and detector settings—are stored with timestamped metadata. Though not inherently 21 CFR Part 11 compliant out-of-the-box, its software architecture supports audit trail export and electronic signature integration when deployed with validated third-party LIMS or ELN platforms.
Software & Data Management
Control and data acquisition are managed through embedded firmware running on a Linux-based real-time OS. The graphical user interface allows full method creation, sequence definition, and post-run analysis without external software dependencies. All chromatograms, collection logs, and system event records are saved in open CSV and PDF formats. Detector signals are sampled at 3 Hz with 16-bit ADC resolution, ensuring sufficient temporal fidelity for accurate peak boundary determination and slope-based triggering. The system supports bidirectional communication with external detectors (RI, ELSD) via RS-232 or analog voltage output, enabling synchronized fraction collection based on multiple orthogonal detection criteria. Exported data conforms to ASTM E1927-20 and ISO/IEC 17025 reporting conventions for traceability and inter-laboratory comparability.
Applications
- Natural product isolation: rapid fractionation of alkaloids, flavonoids, terpenes, and polyphenols from botanical extracts using step-gradient or linear ramp elution.
- Organic synthesis purification: removal of catalysts, ligands, and unreacted starting materials following Suzuki, Heck, or Buchwald–Hartwig reactions.
- Pharmaceutical impurity profiling: isolation of genotoxic impurities and degradation products for structural elucidation by NMR or HRMS.
- Biopolymer characterization: size-fractionation of oligosaccharides and synthetic peptides using aqueous-compatible C18 or diol stationary phases.
- Process scouting: parallel optimization of mobile phase composition, column temperature, and gradient slope prior to scale-up to pilot-scale SFC or prep-HPLC systems.
FAQ
What is the maximum column internal diameter supported by the CHEETAH MP100?
The standard column holder accommodates columns with internal diameters up to 50 mm; optional extended-height brackets support columns up to 80 mm i.d. for higher loading capacity.
Can the system operate with non-UV-absorbing compounds?
Yes—via integration with optional RI or ELSD detectors, enabling detection and fraction-triggering for carbohydrates, lipids, surfactants, and other non-chromophoric analytes.
Is method transfer possible between CHEETAH MP100 and analytical HPLC systems?
Method translation is feasible using retention time normalization and linear velocity scaling; however, due to differences in extra-column volume and detector cell pathlength, empirical re-optimization is recommended for quantitative reproducibility.
Does the system support remote monitoring or network connectivity?
The embedded controller includes Ethernet port and DHCP-enabled TCP/IP stack; remote status viewing and method upload are supported via secure HTTP interface, though live control requires local touchscreen interaction per current firmware revision.
What maintenance intervals are recommended for pump seals and detector flow cells?
Pump seals should be inspected every 500 hours of cumulative operation; detector flow cells require quarterly cleaning with isopropanol followed by water flush to prevent salt crystallization and baseline drift.
