CoMetro CF-2 Fraction Collector

Overview

The CoMetro CF-2 Fraction Collector is a precision-engineered liquid-phase fractionation device designed for integration into preparative HPLC, flash chromatography, and gravity-fed column chromatography systems. It operates on a dual-trigger collection principle—supporting time-based, droplet-count-based, and (when paired with optional flow sensors or volumetric pumps) volume-derived fractionation—enabling reproducible, traceable sample segmentation across analytical and preparative workflows. Its architecture is optimized for low thermal load (<1 W power draw), permitting uninterrupted operation inside refrigerated environments (e.g., cold rooms at 4 °C) without condensation formation or mechanical stress on internal components. Constructed from anodized aluminum and stainless steel, the CF-2 delivers long-term dimensional stability and corrosion resistance in solvent-rich laboratory settings. When synchronized with UV detectors such as the Spectra/Chrom 280, the unit supports peak-triggered collection logic—distinguishing elution peaks from baseline regions and assigning distinct collection parameters (e.g., variable volume per tube) to maximize recovery of target analytes while minimizing dilution and cross-contamination.

Key Features

• Multi-mode collection control: programmable timing (6 sec to 999 min 59 sec, 1-sec resolution), droplet counting (1–9999 drops, 1-drop increment), and external signal triggering via TTL or analog inputs
• Low-inertia tube indexer with 0.35 s switching time for 12/13 mm diameter tubes—ensuring high sample integrity and minimal carryover between fractions
• Modular tube rack system: accommodates up to 174 tubes (12/13 mm), 116 tubes (10–11 mm or 17–18 mm), or 42 flash vials (28 mm)—all interchangeable without tooling
• Integrated drop sensor with adjustable sensitivity and debounce logic—compatible with both low-flow (≤0.1 mL/min) and medium-flow (up to 10 mL/min) applications
• Optional 3-way divert valves (standard and high-flow variants) enable peak-only collection, end-of-run flow diversion, or waste routing—reducing post-run processing burden
• Expandable hardware interface: rear-mounted extension bracket supports detector mounting; 4-column adapter enables parallel collection from up to four independent chromatographic columns (29 fractions per column)
• Passive thermal design: no internal fans or heaters—eliminates vibration-induced column disturbance and ensures compatibility with temperature-sensitive separations

Sample Compatibility & Compliance

The CF-2 is engineered for universal compatibility with commercial and custom-built chromatographic platforms—including systems from Agilent, Waters, Biotage, Teledyne ISCO, and modular lab-assembled setups. Its open I/O architecture accepts TTL-level trigger signals from UV-Vis, fluorescence, conductivity, and mass spectrometric detectors. All mechanical interfaces comply with ISO 8573-1 (compressed air quality) and IEC 61000-6-3 (EMC emission standards). While the instrument itself does not include onboard data logging, its deterministic timing and repeatable actuation support audit-ready workflows when paired with LIMS or chromatography data systems (CDS) validated under 21 CFR Part 11. Tube racks and valve accessories are manufactured to ISO 9001-certified processes and tested for chemical resistance to common organic solvents (acetonitrile, methanol, dichloromethane, ethyl acetate) and aqueous buffers (pH 2–10).

Software & Data Management

The CF-2 operates as a standalone hardware controller with front-panel keypad and LED display—requiring no host PC for basic time- or drop-based collection. For advanced sequencing, it supports RS-232 serial communication (ASCII protocol) to accept remote commands from third-party CDS platforms or custom Python/LabVIEW scripts. Collection event timestamps, tube position indices, and trigger source identifiers (e.g., “UV Peak Start”, “Timer Expired”) can be logged externally via TTL pulse outputs or ASCII stream. When integrated into GxP-regulated environments, users implement electronic records through companion CDS validation packages—leveraging the CF-2’s deterministic behavior to satisfy ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available).

Applications

• Preparative purification of natural products, peptides, and synthetic intermediates via flash or medium-pressure liquid chromatography
• Automated fractionation in metabolomics and proteomics workflows where retention-time-aligned collection improves downstream MS identification confidence
• Method development support: systematic evaluation of gradient dwell volumes, column equilibration times, and solvent strength effects using consistent, operator-independent fraction segmentation
• Quality control of pharmaceutical excipients and active ingredients—collecting discrete fractions for individual assay, residual solvent analysis (GC), or particle size distribution (DLS)
• Teaching laboratories: robust platform for demonstrating chromatographic theory, peak resolution metrics, and fraction pooling strategies without reliance on proprietary software ecosystems

FAQ

Can the CF-2 operate inside a 4 °C cold room without condensation issues?

Yes—the unit draws less than 1 W and contains no internal heat-generating components beyond the stepper motor driver. Its sealed aluminum housing and absence of ventilation ports prevent moisture ingress and eliminate condensation risk.
Is peak-triggered collection possible without a CoMetro UV detector?

Yes—any detector with TTL-compatible output (e.g., analog-to-digital threshold triggers, peak detection pulses) can serve as the external trigger source. Configuration requires matching voltage levels and edge polarity via the rear I/O terminal block.
What is the maximum flow rate supported when using the optional high-flow 3-way valve?

The high-flow variant (P/N 124838) accommodates continuous flow up to 25 mL/min at ≤100 psi, with 3/32″ fluid path and PTFE-sealed rotor for compatibility with aggressive solvents.
Does the CF-2 meet requirements for GLP or GMP documentation?

As a Class I electro-mechanical peripheral, the CF-2 itself is not certified—but its repeatable actuation, traceable firmware versioning, and deterministic response make it suitable for inclusion in validated chromatographic systems meeting GLP (OECD 1998) and GMP (ICH Q7) expectations.
Can I retrofit my existing CF-2 with the 4-column adapter?

Yes—the adapter (P/N 124876) mounts directly to the standard rear extension bracket (P/N 124874); no firmware update or recalibration is required.