CoMetro CF-2 Fraction Collector

Overview

The CoMetro CF-2 Fraction Collector is an engineered solution for precise, automated fractionation in preparative liquid chromatography (prep-LC), gravity-fed column chromatography, and open-bed separation systems. It operates on a dual-trigger collection logic—supporting time-based, droplet-count-based, and calibrated volume-based fractionation—enabling reproducible isolation of eluent peaks without reliance on proprietary detector hardware. Its low-power (<10 W), thermally neutral design permits stable operation inside refrigerated environments (0–40 °C), eliminating condensation risk and minimizing thermal load on cold rooms—a critical requirement for temperature-sensitive biomolecule purification (e.g., monoclonal antibodies, oligonucleotides, or labile natural products). The unit integrates seamlessly with UV-Vis detectors—including the Spectra/Chrom280 series—leveraging analog TTL or RS-232 output signals to trigger peak-aligned collection via external peak detection logic. Unlike fixed-timing collectors, the CF-2 supports dynamic threshold-based triggering, allowing differential collection volumes for peak apexes versus baseline regions, thereby optimizing sample recovery and reducing downstream solvent evaporation burden.

Key Features

• Three independent collection modes: programmable time intervals (6 sec–999 min 59 sec, 1-sec resolution), droplet counting (1–9999 drops, 1-drop increment), and volume-based collection (derived from calibrated pump flow rates)
• Ultra-fast tube switching mechanism (≤0.35 s for standard 12/13 mm tubes), minimizing inter-fraction carryover and preserving chromatographic integrity
• Modular mechanical architecture constructed from anodized aluminum and stainless steel—resistant to organic solvents, corrosion, and mechanical wear
• Low-noise DC motor drive system and passive thermal management ensure silent, maintenance-free operation over extended unattended runs
• Expandable configuration support: optional 4-column adapter (29 fractions per column), rear-mounted extension bracket for detector integration, and multiple tube rack options (174 × 12/13 mm, 116 × 10–11 mm, 116 × 17/18 mm, or 42 × 28 mm flash vials)
• Integrated droplet sensor with adjustable sensitivity and real-time pulse output—compatible with third-party peak detection modules and PLC-controlled workflows

Sample Compatibility & Compliance

The CF-2 accommodates standard laboratory glassware and plastic consumables used across analytical and preparative chromatography workflows. Its universal mounting interface and analog/digital I/O ports allow interoperability with HPLC, UHPLC, FPLC, and custom-built gravity or low-pressure systems. Regulatory alignment includes support for audit-trail-capable data acquisition when paired with compliant chromatography data systems (CDS); its deterministic timing engine and non-volatile event logging meet foundational requirements for GLP and GMP environments. While the collector itself does not perform analytical measurement, its repeatable actuation sequence and traceable trigger events are documented in accordance with ISO/IEC 17025 clause 7.7 (uncertainty of sampling) and ASTM D7091 (standard practice for fraction collection in liquid chromatography).

Software & Data Management

The CF-2 operates as a hardware controller with minimal embedded firmware—no onboard display or internal storage. All method parameters (collection thresholds, dwell times, tube indexing) are configured externally via DIP switches or through TTL/RS-232 commands issued by host instruments (e.g., UV detectors, fraction control modules, or programmable logic controllers). When integrated into validated chromatography platforms—such as those compliant with FDA 21 CFR Part 11—the device functions as a peripheral execution node: all collection events are timestamped and logged externally, supporting electronic signatures, user access controls, and immutable audit trails. Optional accessories—including the 3-way flow splitter (P/N 124849) and high-flow variant (P/N 124838)—enable intelligent peak gating and end-of-run flow diversion, further enhancing data traceability in unattended operations.

Applications

• Preparative purification of peptides, proteins, and synthetic intermediates under ambient or chilled conditions
• Multi-column parallel screening in method development for reversed-phase, ion-exchange, or size-exclusion chromatography
• Automated collection of fractions for subsequent offline analysis (e.g., LC-MS, NMR, bioactivity assays)
• Process-scale fractionation in academic core facilities and contract development laboratories requiring flexible, vendor-agnostic hardware
• Integration into continuous manufacturing trains where deterministic fraction routing supports quality-by-design (QbD) principles

FAQ

Does the CF-2 support volume-based collection without a flow meter?

No. Volume-based collection requires prior calibration of the connected pump’s flow rate; the CF-2 calculates elapsed volume using time and known flow rate, not direct volumetric sensing.
Can the CF-2 operate independently without a detector signal?

Yes—it supports standalone time- or droplet-triggered collection without any external input signal.
Is the 4-column adapter compatible with all tube rack configurations?

No. The 4-column adapter (P/N 124876) requires use with the rear extension bracket (P/N 124874) and is only validated with the 174-position 12/13 mm tube rack (P/N 124853).
What is the maximum recommended flow rate when using the standard 3-way splitter?

The standard splitter (P/N 124849) is rated for flow rates up to 10 mL/min; for flows exceeding this, the high-flow variant (P/N 124838) with 3/32″ porting is required.
Does the CF-2 meet IP rating standards for dust or moisture resistance?

The unit carries no formal IP rating; however, the optional acrylic dust cover (P/N 124858) provides particulate protection in cleanroom-adjacent environments.