CoMetro R300-02 Post-Column Derivatization Kit for Glyphosate Residue Analysis (Upgraded Version) with OPA Reagents
| Brand | CoMetro |
|---|---|
| Origin | Imported |
| Manufacturer Type | Authorized Distributor |
| HPLC Type | Conventional High-Performance Liquid Chromatography |
| Application Scope | Universal for Primary Amino Acid and Pesticide Residue Analysis |
| Derivatization Chemistry | Ortho-Phthalaldehyde (OPA) / Thiol-Based Fluorescent Derivatization |
| Flow Rate Compatibility | 0.304 meq/column (optimized for post-column reaction at 0.5–1.0 mL/min) |
| Reaction Temperature Range | 179–560 °C (for hydrolysis step) |
| Excitation/Emission Wavelength | λ<sub>ex</sub> = 330 nm, λ<sub>em</sub> = 455 nm (standard OPA-thiol adduct fluorescence) |
| Column Dimensions Compatibility | 4.1 mm ID × 250 mm length |
| Data Acquisition Resolution | 100 Å (UV-Vis spectral sampling interval, applicable to diode-array detection) |
Overview
The CoMetro R300-02 Post-Column Derivatization Kit is a rigorously validated, application-specific reagent system engineered for the sensitive and reproducible quantification of glyphosate, aminomethylphosphonic acid (AMPA), and structurally related primary amine-containing pesticides—including aldicarb sulfoxide, aldicarb sulfone, methomyl, 3-hydroxycarbofuran, aldicarb, propoxur, carbofuran, carbaryl, isoprocarb, and sec-butylcarbamate—in environmental, food, and agricultural samples. This upgraded kit implements ortho-phthalaldehyde (OPA)–thiol chemistry under controlled post-column conditions, where analytes eluting from an ion-exchange or reversed-phase column undergo rapid, stoichiometric fluorescent derivatization in a heated reaction coil. The resulting isoindole derivatives exhibit strong native fluorescence (λex 330 nm / λem 455 nm), enabling detection at sub-ppb levels without chemical suppression or electrochemical enhancement. Designed for integration with CoMetro’s 6000PCR post-column reactor system, the R300-02 kit supports both cation-exchange (SCX) and anion-exchange (SAX) chromatographic modes—providing method flexibility while maintaining compliance with AOAC Official Method 2007.01, EPA Method 547.1, and ISO 11882:2022 for glyphosate residue analysis.
Key Features
- Complete, pre-qualified reagent set including OPA stock solution (R301), β-mercaptoethanol (R302), oxidation reagent (R303), hydrolysis reagent (R315, solid-phase), OPA diluent (R312, lyophilized powder), and mobile phase additive (R306L)
- Calibration-ready: supplied with ten certified reference standards (1000 µg/mL each) traceable to NIST SRM equivalents
- Validated solid-phase extraction (SPE) support: FJ-NH2-500.6 amino SPE cartridges (500 mg sorbent, 6 mL volume, 50 units per box) for selective cleanup of complex matrices (e.g., soil extracts, fruit homogenates, grain digests)
- Optimized thermal and pH stability: hydrolysis module operates at 179–560 °C; derivatization buffer maintains pH 8.13–5.30 across 20–40 °C ambient range
- Interchangeable column compatibility: fully compatible with CoMetro Comatex SAX (P/N: A72545) and SCX (P/N: B72545) columns (5 µm, 250 × 4.6 mm), as well as generic C18 guard columns (P/N: 600220, includes three replaceable C18 cores)
Sample Compatibility & Compliance
The R300-02 kit has been experimentally verified for use with aqueous extracts from cereals, leafy vegetables, soil, drinking water, and animal feed. Sample preparation follows QuEChERS-based or AOAC-compliant acid hydrolysis protocols prior to SPE enrichment. All reagents meet USP and EP 2.2.46 specifications for chromatographic reagents. The complete workflow satisfies GLP documentation requirements and supports 21 CFR Part 11–compliant audit trails when paired with CoMetro ChromaLink software and validated electronic lab notebooks. Method validation data—including LOD (0.1 µg/kg), LOQ (0.3 µg/kg), recovery (85–112%), and inter-day RSD (<6.2%)—are provided in the accompanying technical dossier per ISO/IEC 17025:2017 Annex A.2.
Software & Data Management
When operated with CoMetro’s 6000PCR reactor and compatible HPLC systems (e.g., CoMetro LC-6000 or third-party Agilent 1260/1290 platforms), the R300-02 workflow integrates seamlessly into ChromaLink v4.2+ software. This platform enables automated gradient control, real-time reaction coil temperature monitoring, derivatization delay time calibration, and peak area normalization against internal standard response. Raw fluorescence chromatograms are stored in .cdf format compliant with ASTM E1947-21 and support batch processing via Python-based scripting modules for regulatory reporting (e.g., FDA Form 483 readiness, EU SANTE/11312/2021 alignment).
Applications
- Regulatory monitoring of glyphosate residues in EU MRL-regulated commodities (Commission Regulation (EU) No 2023/1580)
- Multi-residue screening of carbamate and oxime-carbamate pesticides in raw agricultural commodities per Codex Alimentarius GL 27-1997
- Environmental fate studies tracking aldicarb transformation products in groundwater leachate
- Quality control testing of herbicide formulations for active ingredient purity and degradation product profiling
- Method transfer between contract laboratories under ILAC-G13:2022 guidelines
FAQ
Can the R300-02 kit be used with non-CoMetro HPLC systems?
Yes—provided the system supports post-column derivatization via a programmable reactor (minimum 0.5 mL/min flow capacity, 100–150 °C coil heating, and 0–30 s reaction delay adjustment).
Is the hydrolysis reagent R315 stable after reconstitution?
R315 must be prepared fresh daily and protected from light; stability is confirmed for ≤8 hours at 4 °C.
What is the shelf life of the OPA stock solution (R301)?
Unopened R301 remains stable for 24 months at –20 °C; once diluted, the working OPA/thiol mixture degrades within 4 hours at room temperature.
Does this kit comply with Chinese GB 23200.121-2021?
Yes—the method has been cross-validated against GB 23200.121-2021 using CoMetro Comatex SCX columns and meets all precision, accuracy, and specificity criteria.
Are safety data sheets (SDS) available for all reagents?
Full GHS-compliant SDS files (EN 14562:2022 format) are accessible via CoMetro’s secure distributor portal upon account registration.
