CSY-JAZ Deoxynivalenol (DON) Rapid Immunoassay Analyzer for Wheat Flour and Cereal Commodities

Overview

The CSY-JAZ Deoxynivalenol (DON) Rapid Immunoassay Analyzer is an optical reflectometric instrument engineered for the semi-quantitative and quantitative determination of deoxynivalenol—a type B trichothecene mycotoxin—in wheat flour, milled cereals, corn, rice, barley, oats, peanut meal, and associated feedstuffs. It operates on the principle of lateral flow immunoassay (LFIA) strip scanning, where a precisely calibrated LED light source (450–475 nm) illuminates the test and control lines on immunochromatographic strips. The analyzer captures reflected light intensity via a photodiode array and computes analyte concentration based on the attenuation ratio between test (T) and control (C) line signals. This photometric method delivers rapid, field-deployable results without requiring enzymatic amplification or laboratory-grade infrastructure—making it suitable for decentralized screening at grain intake points, feed mills, regulatory checkpoints, and quality assurance labs operating under time-sensitive workflows.

Key Features

• High-throughput capacity: Up to 240 independent DON assays per hour, enabled by automated strip positioning and real-time reflectance signal acquisition.
• Robust optical architecture: Fixed-wavelength LED illumination (450–475 nm) ensures spectral consistency across instruments; photometric repeatability ≤1% CV using NIST-traceable reference strips.
• Flexible hardware configuration: Standard single-channel design supports scalable expansion to dual-, 5-, or 10-channel modules for parallel sample processing—ideal for batched commodity screening.
• Intelligent line recognition engine: Adaptive C/T line detection with ±3 mm positional tolerance; user-adjustable line width and inter-line spacing parameters accommodate diverse strip formats and manufacturing variances.
• Integrated connectivity suite: Native USB 2.0, RS-232 serial, and 10/100M Ethernet ports enable direct LIMS or ERP integration; optional Wi-Fi module supports secure over-the-air firmware updates and remote diagnostics.
• Compliance-enabling software architecture: Built-in database stores raw scan curves, concentration values (µg/kg), timestamps, operator IDs, and strip lot numbers—supporting audit-trail generation and 21 CFR Part 11–compliant electronic records when paired with validated software licenses.

Sample Compatibility & Compliance

The CSY-JAZ system is validated for use with commercially available DON-specific lateral flow test strips compliant with AOAC PTM #080901 and ISO 15737:2003 (immunoassay performance criteria). Sample preparation follows standardized extraction protocols (e.g., EN 15871:2010 for cereals) prior to dilution and application onto strips. Instrument performance meets inter-laboratory precision targets outlined in EU Commission Regulation (EC) No 401/2006 for DON in unprocessed cereals (LOQ = 200 µg/kg). All firmware and calibration routines are traceable to manufacturer-certified reference standards; device documentation supports GLP/GMP-aligned validation packages including IQ/OQ/PQ protocols.

Software & Data Management

The embedded Linux-based operating system provides intuitive touch-driven navigation through assay setup, calibration management, result review, and report generation. Each test record includes metadata: strip lot number, expiration date, operator ID (via optional RFID/NFC card authentication), environmental temperature/humidity (if external sensor connected), and full-resolution reflectance scan curves. Data export options include CSV, PDF, and XML formats compatible with enterprise LIMS platforms. Optional software modules provide trend analysis, statistical process control (SPC) charts, and automated alerting for out-of-specification results—facilitating proactive risk mitigation in supply chain monitoring.

Applications

• Routine incoming inspection of wheat flour and cereal raw materials at milling and baking facilities.
• On-site DON screening at grain elevators, cooperatives, and port quarantine laboratories.
• Feed safety verification in compound feed plants and premix manufacturers supplying livestock operations.
• Regulatory surveillance by national food safety authorities conducting market surveillance programs under EC No 1881/2006.
• Research applications supporting mycotoxin exposure modeling and intervention efficacy studies in agronomic trials.

FAQ

What sample types are validated for use with the CSY-JAZ system?

Wheat flour, whole wheat, corn grits, rice, barley, oats, peanut meal, soybean meal, and finished animal feed formulations—following EN 15871:2010 or AOAC 2005.05 extraction protocols.
Does the instrument require daily recalibration?

No. Factory-installed calibration remains stable for ≥6 months under normal operating conditions; optional daily self-check using control strips is supported.
Can the CSY-JAZ interface with enterprise laboratory information systems?

Yes. Native Ethernet and USB interfaces support HL7, ASTM E1384, and custom API integrations; Wi-Fi-enabled remote data push is configurable.
Is the device suitable for field deployment in mobile laboratories or grain trucks?

Yes. 12 V DC input enables direct connection to vehicle power systems; optional internal Li-ion battery extends operation to >8 hours without external power.
How is data integrity ensured during regulatory audits?

All measurements are timestamped, operator-identified, and stored with immutable raw scan curves; optional 21 CFR Part 11 software package adds electronic signature, role-based access, and audit-log encryption.