CZGY GYHZ-3X Stackable Constant-Temperature Orbital Shaker

Overview

The CZGY GYHZ-3X Stackable Constant-Temperature Orbital Shaker is an engineered solution for laboratories requiring high-density, multi-zone incubation and agitation in constrained spatial environments. Designed around the principle of orbital shaking—where a circular motion induces gentle yet effective mixing of liquid cultures, suspensions, or biochemical reactions—the instrument integrates precise temperature control (4–60 °C) with independent rotational actuation across three vertically stacked platforms. Each platform operates under fully decoupled microprocessor-based PID control, enabling simultaneous experiments at distinct thermal and kinetic conditions within a single footprint. The system employs a five-dimensional eccentric triple-cam drive mechanism to ensure mechanical stability, minimal vibration transmission, and long-term operational reproducibility—critical for sensitive cell culture, microbial growth, enzyme kinetics, and protein expression workflows. Its sealed, insulated chamber with double-glazed observation window maintains thermal integrity while permitting real-time visual monitoring without compromising environmental consistency.

Key Features

• Three independently controlled orbital platforms (930 × 550 mm total footprint), each configurable with customizable clamp arrays for vessels ranging from 250 mL to 2000 mL (e.g., 60 × 250 mL flasks per layer)
• Orbital motion with 26 mm amplitude and programmable frequency range (30–300 rpm), optimized for aerobic culture aeration and homogeneous suspension without shear-induced cellular damage
• High-efficiency cooling architecture: R134a-free refrigerant system with electronic expansion valve, defrost valve, and pressure relief valve—enabling continuous compressor operation down to 4 °C with <±0.5 °C uniformity across all platforms
• 4.3″ full-color TFT touchscreen interface with intuitive graphical navigation; optional 7″ upgrade supports multi-touch gesture control and expanded data visualization
• Real-time data acquisition and storage via integrated USB logging: continuous timestamped records of temperature, speed, elapsed time, alarms, and diagnostic events—exportable as CSV and automatically rendered as overlayable trend curves
• UV-C sterilization subsystem with dual functionality: upper-chamber irradiation for surface decontamination and dedicated air duct UV exposure to suppress airborne microbial load between runs
• Safety-engineered interlocks: automatic motor stop upon door opening, leakage current detection (<0.5 mA trip threshold), overcurrent circuit interruption, and self-diagnostic reporting with ISO/IEC 61000-4-5 compliant fault code indexing
• Modular mechanical design: front-access sliding trays (40 cm extension) for ergonomic sample loading/unloading; cold-rolled steel frame with electrostatic powder coating; mirror-finish 304 stainless steel interior resistant to corrosion and biofilm adhesion

Sample Compatibility & Compliance

The GYHZ-3X accommodates standard microbiological and bioprocess vessels—including Erlenmeyer flasks, baffled bottles, and culture tubes—via interchangeable clamp fixtures (customizable per user specification). Its orbital agitation profile meets ASTM D2510 and ISO 8573-1 requirements for consistent mixing energy input in aerobic fermentation studies. Temperature control accuracy (±0.1 °C resolution, ±0.5 °C uniformity) aligns with USP guidelines for cell culture environment qualification. The device’s data logging architecture supports ALCOA+ principles: attributable, legible, contemporaneous, original, accurate, complete, consistent, enduring, and available—facilitating compliance with FDA 21 CFR Part 11 when paired with validated USB media handling protocols. All electrical safety certifications conform to IEC 61010-1:2010 for laboratory equipment.

Software & Data Management

The embedded firmware implements a deterministic real-time operating system (RTOS) with non-volatile memory allocation for persistent parameter retention across power cycles. Experimental logs are stored on plug-and-play USB drives in FAT32 format, with automatic file naming (YYYYMMDD_HHMMSS_GYHZ3X_LOG.csv) and cyclic overwrite protection. On-screen visualization includes synchronized dual-axis plots (temperature vs. time, rpm vs. time), zoomable historical traces, and annotated alarm markers (e.g., “T1-Overtemp@14:22:08”). Data export supports direct import into MATLAB, Python (pandas), or LIMS platforms via standardized timestamped columns. Optional Wi-Fi module enables remote status polling and parameter adjustment through HTTPS-secured REST API endpoints—audit-trail enabled per GLP/GMP Annex 11 expectations.

Applications

• Microbial cultivation (E. coli, yeast, Bacillus spp.) under controlled O₂ transfer conditions
• Mammalian and insect cell line expansion in serum-free or low-serum media
• Enzyme assay development requiring precise thermal and kinetic co-variation
• Protein solubilization and refolding studies with gradient temperature ramping
• Environmental microbiology: biodegradation kinetics, biofilm formation assays, and antimicrobial susceptibility testing
• Biopharmaceutical upstream process development, including clone screening and media optimization
• Academic teaching labs: scalable demonstration of exponential growth phases, Monod kinetics, and mass transfer limitations

FAQ

Can the GYHZ-3X operate with different shaking modes on each platform?

Yes—each of the three platforms supports independent selection between orbital and reciprocating motion (optional retrofit), allowing concurrent experiments with distinct agitation profiles.
Is validation documentation provided for IQ/OQ/PQ protocols?

CZGY supplies factory calibration certificates traceable to NIST standards, along with a comprehensive User Requirements Specification (URS) template and test protocol outlines aligned with ISO 13485 and EU GMP Annex 15.
What is the maximum ambient temperature for stable 4 °C operation?

For sustained sub-ambient performance, ambient conditions must remain ≤25 °C with ≥15 cm rear clearance and unrestricted airflow around condenser coils.
How does the UV sterilization cycle integrate with temperature control?

UV lamps activate only during idle periods (when temperature and agitation are disabled) and deactivate automatically if door is opened or chamber temperature exceeds 40 °C—preventing ozone generation and material degradation.
Are third-party LIMS integrations supported?

Yes—through optional RS-485 Modbus RTU or Ethernet TCP/IP interfaces (available upon request), enabling bidirectional communication with major laboratory informatics systems under ASTM E1384-compliant messaging schemas.