DELEITE V2.5 Adjustable-Volume Single-Channel Manual Pipette

Overview

The DELEITE V2.5 is a high-precision, manually operated single-channel adjustable-volume pipette engineered for reliable liquid handling in analytical, quality control, and research laboratory environments. It operates on the air displacement principle—where piston-driven air compression creates controlled aspiration and dispensing of liquids—ensuring consistent volumetric delivery across its full 1–10 mL range. Designed for routine and semi-quantitative applications requiring robust mechanical performance and traceable calibration, the V2.5 integrates ergonomic engineering with ISO 8655-compliant metrological design. Its stainless-steel internal mechanism, chemically resistant PTFE-coated seals, and autoclavable upper body (up to 121 °C, 20 min) support long-term stability under repeated sterilization cycles. Unlike electronic or multi-channel alternatives, the V2.5 prioritizes mechanical simplicity, minimizing failure points while maintaining reproducibility suitable for GLP-aligned workflows.

Key Features

• Manually actuated piston mechanism with calibrated spring tension and low-force plunger travel for reduced operator fatigue during high-throughput tasks
• Adjustable volume setting via dual-scale graduated dial: primary scale (mL) and fine-tuning vernier scale (0.1 mL increments), enabling precise manual selection without digital interface dependency
• Universal tip compatibility with standard conical 10 mL pipette tips (ISO 8655-3 compliant); tip ejection force optimized to prevent accidental tip loss or leakage
• Corrosion-resistant anodized aluminum housing with non-slip textured grip zone; weight-balanced design (142 g) reduces wrist strain during extended use
• Calibration-friendly construction: two-point adjustment accessible via hex-key service port supports in-house verification against gravimetric standards per ISO 8655-6
• Compliance-ready documentation package includes factory calibration certificate (traceable to NIM, China), user manual with maintenance protocols, and optional IQ/OQ templates for regulated labs

Sample Compatibility & Compliance

The V2.5 delivers accurate and repeatable transfer of aqueous solutions, buffers, diluted acids/bases, and low-viscosity organic solvents (e.g., ethanol, acetone, methanol) within its specified operating temperature range (15–30 °C) and relative humidity (30–70% RH). It is not recommended for highly volatile, viscous (>100 mPa·s), or corrosive media (e.g., concentrated HCl, HF, or DMSO above 50% v/v) without pre-wetting and tip conditioning. The pipette conforms to ISO 8655 Parts 1–6 for piston-operated volumetric apparatus, including requirements for accuracy, repeatability, thermal drift, and mechanical durability. While not FDA 21 CFR Part 11–certified (as it lacks electronic audit trail functionality), its calibration traceability and serviceability align with GMP Annex 15 and EU GMP Chapter 4 expectations for manual equipment used in supporting analytical operations.

Software & Data Management

As a fully manual instrument, the V2.5 does not incorporate embedded electronics, firmware, or data logging capabilities. All operational parameters—including volume setting, tip type, and calibration history—are documented externally using laboratory-controlled paper-based or LIMS-integrated records. Users are advised to maintain calibration logs per ISO/IEC 17025:2017 Clause 6.4.10, including date, technician ID, reference standard used (e.g., Class A volumetric flask or certified weight), deviation values, and pass/fail determination. Optional DELEITE Calibration Verification Kit (Cat. No. CAL-V2.5-KIT) includes certified weights, distilled water, analytical balance protocol, and editable Excel-based reporting templates compatible with common LIMS platforms.

Applications

• Dilution series preparation in spectrophotometric and ELISA assay workflows
• Standard solution dispensing for HPLC and GC calibration curves (volumes ≥2 mL)
• Media and reagent aliquoting in microbiology and cell culture laboratories
• Sample transfer in environmental testing (e.g., wastewater nutrient analysis per APHA 4500-NH₃)
• QC sampling in pharmaceutical manufacturing where manual volumetric transfer is permitted under SOP-defined limits
• Teaching laboratories emphasizing fundamental pipetting technique, error source identification, and gravimetric validation exercises

FAQ

Is the V2.5 autoclavable?
Yes—the upper body (excluding plunger cap and tip cone seal) is rated for autoclaving at 121 °C for 20 minutes. Disassembly per Section 4.2 of the user manual is required prior to sterilization.
What is the recommended recalibration interval?
Per ISO 8655-6 and typical lab practice, recalibration is advised every 3–6 months for daily use, or after 10,000 actuations—whichever occurs first. More frequent verification is required if used with aggressive solvents or in high-humidity environments.
Can the V2.5 be used with positive-displacement tips?
No. The V2.5 is designed exclusively for air-displacement operation with conical universal tips. Positive-displacement systems require dedicated piston-tip assemblies incompatible with this model.
Does DELEITE provide third-party calibration certificates?
Yes—accredited calibration services (CNAS-certified labs) are available upon request. Certificates include measurement uncertainty budgets and traceability statements aligned with ILAC MRA requirements.
How is accuracy verified gravimetrically?
Using distilled water at 20 °C, dispense 10 replicates into a tared Class A weighing boat on a calibrated analytical balance (±0.1 mg resolution). Calculate mean mass, convert to volume using water density (0.998203 g/mL), and compare against set volume per ISO 8655-6 Annex B.