DLAB TopPette Single-Channel Adjustable-Volume Pipette
| Brand | DLAB |
|---|---|
| Origin | Beijing, China |
| Model | 2–20 µL |
| Accuracy (±) | 0.9% |
| Precision (CV) | 0.4% |
| Volume Adjustment Increment | 0.5 µL |
| Tip Compatibility | 300 µL universal low-retention tips |
| Sterilization | Autoclavable (121 °C, 20 min) |
| Regulatory Compliance | ISO 8655-2:2022, GLP-ready design |
Overview
The DLAB TopPette Single-Channel Adjustable-Volume Pipette is an ISO 8655-2:2022 compliant manual liquid handling instrument engineered for precision, repeatability, and ergonomic sustainability in high-throughput laboratory environments. Utilizing a positive displacement air-cushion mechanism with a calibrated spring-loaded piston and sealed O-ring system, the TopPette delivers traceable volumetric accuracy across its full 2–20 µL range. Its design adheres to fundamental metrological principles defined in ISO 8655 for piston-operated volumetric apparatus—ensuring consistent aspiration and dispense performance under variable ambient conditions (20–25 °C, 40–60% RH). The pipette is optimized for applications requiring strict adherence to Good Laboratory Practice (GLP) and supports audit-ready documentation workflows through standardized calibration intervals and traceable adjustment records.
Key Features
- Ergonomic grip geometry with contoured finger rests and textured thumb wheel—reducing operator hand fatigue during extended use sessions (validated per ISO 7250-1 anthropometric guidelines).
- High-contrast digital volume window with dual-scale readout (µL and fractional increments), enabling rapid verification without parallax error.
- Volume lock mechanism prevents accidental setting drift during transport or benchtop operation—critical for assay reproducibility in QC/QA workflows.
- Modular lower assembly: fully detachable tip cone with chemically resistant PTFE-coated stainless steel components, compatible with autoclaving at 121 °C for 20 minutes (EN 285-compliant cycles).
- Integrated filter barrier system (replaceable hydrophobic PTFE filter cartridge) protects internal mechanisms from aerosol contamination and sample carryover.
- One-touch tip ejection with low-force actuation (≤1.2 N), minimizing wrist strain and ensuring consistent tip release across repeated cycles.
- Tool-free calibration access via side-panel service port—enabling in-lab adjustment using standard gravimetric procedures per ISO 8655-6.
Sample Compatibility & Compliance
The TopPette 2–20 µL model is validated for aqueous solutions, buffers, diluted acids/bases (pH 2–12), and common organic solvents including ethanol, isopropanol, and acetonitrile—provided tip compatibility is maintained. It meets ISO 8655-2:2022 requirements for accuracy (±0.9%) and precision (CV ≤ 0.4%) at nominal volume (20 µL), with linearity verified across 10%, 50%, and 100% of range. All materials contacting liquids comply with USP Class VI biocompatibility standards. Device construction excludes phthalates, latex, and heavy metals per RoHS Directive 2011/65/EU. Documentation packages include factory calibration certificates traceable to NIM (National Institute of Metrology, China) and support 21 CFR Part 11–compatible electronic record retention when paired with DLAB’s optional calibration management software.
Software & Data Management
While the TopPette is a manually operated device, its calibration history, maintenance logs, and performance verification data integrate seamlessly into laboratory quality management systems (QMS). DLAB provides a free downloadable Calibration Log Template (XLSX format) aligned with ISO/IEC 17025 clause 7.7 and GLP Annex III requirements. For regulated environments, users may import pipette-specific metadata—including serial number, date of last verification, technician ID, and gravimetric test results—into LIMS platforms via CSV export. Optional QR-coded asset labels facilitate barcode-scanned entry into inventory tracking modules. No embedded firmware or cloud connectivity is present; all data sovereignty remains under institutional control.
Applications
This pipette is routinely deployed in molecular biology workflows (cDNA synthesis, qPCR master mix preparation), clinical diagnostics (serum dilution series, ELISA plate loading), pharmaceutical QC testing (content uniformity assays, dissolution sampling), and academic research involving microscale reagent dispensing. Its 0.5 µL increment resolution and tight CV make it suitable for low-volume applications such as CRISPR guide RNA complex formation, single-cell lysate transfer, and nanoliter-range standard curve preparation. Compatibility with low-retention 300 µL tips ensures minimal surface adsorption of proteins and viscous biomolecules (e.g., BSA, genomic DNA >50 kb).
FAQ
Is the TopPette 2–20 µL autoclavable?
Yes—the entire lower assembly (tip cone, piston sleeve, and filter housing) is rated for 121 °C, 20-minute autoclave cycles. The upper body must be disassembled prior to sterilization.
What calibration standards are recommended?
Gravimetric calibration per ISO 8655-6 using certified Class E2 weights, distilled water (density corrected for local temperature/humidity), and a calibrated analytical balance (≥0.01 mg readability).
Can this pipette be used with reverse pipetting techniques?
Yes—its positive-displacement architecture and adjustable plunger resistance allow reliable reverse pipetting for viscous or volatile liquids, provided tip immersion depth and pause times follow ISO 8655-5 guidance.
Does DLAB provide certified calibration services?
Yes—DLAB-certified service centers offer accredited calibration (ISO/IEC 17025) with NIST-traceable certificates, including uncertainty budgets per ISO/IEC 17025:2017 clause 7.6.3.
How often should routine verification be performed?
Daily pre-use checks are recommended for critical applications; formal calibration intervals depend on usage frequency and risk assessment—but typically range from 3 to 6 months per ISO/IEC 17025 and CLSI EP15-A3 guidelines.
