Elite EClassical 3200 High-Performance Liquid Chromatograph (HPLC System)
| Brand | Elite |
|---|---|
| Origin | Liaoning, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Origin | Domestic (China) |
| Model | EClassical 3200 |
| Price Range | USD 14,000–43,000 (FOB) |
| Instrument Type | Conventional HPLC System |
| Application Scope | General-purpose analytical chromatography |
| Flow Rate Range | 0.001–10.000 mL/min |
| Maximum Operating Pressure | 90 MPa |
| Autosampler Capacity | Not integrated (manual injection only) |
| Injection Volume Range | Not specified (compatible with standard 10–100 µL fixed-loop or syringe-based manual injectors) |
| Column Oven Temperature Control | Not built-in (external oven required for temperature-sensitive separations) |
| UV-Vis Detection Wavelength Range | Detector not included in base configuration (requires optional UV/Vis or PDA detector module) |
| Data Acquisition Frequency | Dependent on connected detector and software sampling settings |
Overview
The Elite EClassical 3200 is a modular, high-pressure liquid chromatography system engineered for robustness, method reproducibility, and operational flexibility in routine analytical laboratories. Designed according to core principles of isocratic and gradient elution chromatography, the system employs dual high-precision plunger pumps with active solvent compressibility compensation to deliver stable flow rates across its full range of 0.001–10.000 mL/min. Its 90 MPa maximum pressure rating supports use with sub-2 µm particle-packed columns and UHPLC-compatible stationary phases—enabling high-resolution separations without requiring ultra-high-pressure instrumentation. The EClassical 3200 operates as a base platform: it does not include an integrated autosampler, column oven, or detector; instead, it is configured to interface with third-party or Elite-supplied detection modules (e.g., UV-Vis, photodiode array, fluorescence), external column thermostats, and manual or automated sample introduction systems. This architecture allows laboratories to scale functionality based on application complexity, regulatory requirements, and budget constraints—making it suitable for QC release testing, academic research, method development, and stability-indicating assays.
Key Features
- High-accuracy binary or quaternary solvent delivery system with real-time pressure monitoring and automatic leak detection
- Flow rate precision ≤ ±0.1% RSD and accuracy ±0.2% over full range (0.001–10.000 mL/min), validated per ISO 11607-2 and ASTM D5298 protocols
- Stainless-steel fluidic path with chemically resistant pump heads and check valves rated for aqueous, organic, and corrosive mobile phases (e.g., TFA, HFBA)
- Modular design compliant with IEC 61000-6-3 (EMC) and IEC 61010-1 (safety); CE-marked for laboratory equipment
- Front-panel LCD interface with intuitive menu navigation and real-time system status display (pressure, flow, valve position)
- Digital communication interfaces: RS-232, USB 2.0, and optional Ethernet (TCP/IP) for integration into LIMS or centralized instrument management platforms
Sample Compatibility & Compliance
The EClassical 3200 accommodates standard 1/16″ stainless-steel or PEEK capillary tubing, 4.6 mm ID analytical columns (up to 250 mm length), and guard cartridges compatible with reversed-phase (C18, C8), HILIC, ion-exchange, and size-exclusion chemistries. As a Class II analytical instrument under China’s JJG 705–2014 verification regulation, it meets baseline performance criteria for retention time repeatability (<0.2% RSD, n=6) and peak area precision (<1.5% RSD, n=6) when paired with a certified UV detector. For regulated environments—including pharmaceutical QC labs operating under GMP or GLP—the system supports audit trail-enabled software (via optional Elite ChromaLink v3.x) compliant with FDA 21 CFR Part 11 requirements, including electronic signatures, user access levels, and immutable data archiving.
Software & Data Management
ChromaLink software (v3.2 or later) provides full instrument control, gradient programming, peak integration, calibration curve generation, and report export (PDF, CSV, XML). It features embedded validation templates aligned with USP , EP 2.2.46, and ICH Q2(R2) guidelines. Raw data files are stored in vendor-neutral .cdf format (based on NetCDF 4.3.3), ensuring long-term readability and compatibility with third-party analysis tools such as OpenChrom or ACD/Labs. All acquisition and processing events—including method changes, integration parameter edits, and reprocessing actions—are timestamped and logged with operator ID, satisfying ALCOA+ data integrity principles.
Applications
- Pharmaceutical impurity profiling and assay quantification per ICH Q5A and Q6A
- Food additive and pesticide residue analysis per AOAC 2012.01 and EU SANTE/11312/2023
- Environmental water testing for PAHs, phenols, and pharmaceuticals (EPA Method 8330B)
- Biopolymer characterization using SEC-MALS coupling (with optional multi-angle light scattering detector)
- Academic teaching labs requiring configurable, serviceable HPLC platforms for chromatography fundamentals instruction
FAQ
Does the EClassical 3200 include a detector?
No—the base system is a pump and controller only. UV-Vis, PDA, fluorescence, or mass spectrometry detectors must be selected separately and installed externally.
Can it be used for UHPLC methods?
Yes, provided column dimensions and particle size are compatible with 90 MPa pressure limits; however, dwell volume and system dispersion may affect resolution at >600 bar—method transfer requires gradient delay characterization.
Is GLP-compliant data handling supported?
Yes, when configured with ChromaLink v3.2+ and enabled audit trail, electronic signature, and role-based access controls.
What maintenance intervals are recommended?
Pump seal replacement every 6 months under continuous operation; inlet filter cleaning weekly; annual calibration verification using NIST-traceable standards.
Is remote monitoring available?
Yes, via optional Ethernet module and Elite’s WebMonitor cloud interface (HTTPS-secured), enabling real-time pressure/flow telemetry and alarm notifications.
