Elite EClassical 3200L Fast Liquid Chromatograph
| Brand | Elite |
|---|---|
| Origin | Liaoning, China |
| Manufacturer Type | Direct Manufacturer |
| Instrument Type | Fast Liquid Chromatograph |
| Flow Rate Range | 0.001–10.000 mL/min |
| Maximum Operating Pressure | 130 MPa |
| Flow Accuracy | ≤0.05% RSD |
| Column Oven Temperature Range | 5–85 °C |
| UV-Vis Wavelength Range | 190–800 nm |
| Data Acquisition Rate | 2–40 Hz |
Overview
The Elite EClassical 3200L Fast Liquid Chromatograph is an engineered solution for laboratories requiring high-resolution, high-throughput separations under elevated backpressure conditions. Built upon a robust dual-plunger reciprocating pump architecture with active solvent compressibility compensation, the system operates on the principle of high-pressure liquid chromatography (HPLC) with scalability toward ultra-high-performance liquid chromatography (UHPLC)-class performance. Its 130 MPa maximum pressure rating enables routine use of sub-2 µm fully porous silica and superficially porous (core-shell) stationary phases—delivering enhanced peak capacity, reduced analysis time, and improved mass sensitivity without sacrificing retention time reproducibility or baseline stability. Designed for method development agility and regulatory-compliant operation, the EClassical 3200L integrates hardware-level precision control with software-driven workflow automation to support both routine QC and exploratory research applications.
Key Features
- High-pressure binary gradient pump with real-time flow calibration and ≤0.05% RSD flow accuracy across 0.001–10.000 mL/min range
- Column oven with three independent thermal control modes: heating-only, cooling-only, and intelligent hybrid mode—maintaining ±0.1 °C temperature stability from 5 to 85 °C
- UV-Vis detector featuring a high-stability deuterium/tungsten lamp source and 190–800 nm spectral range with 1 nm wavelength accuracy and 2–40 Hz data acquisition flexibility
- Autosampler with low-dead-volume injection valve and integrated needle wash station—achieving <0.005% carryover for trace-level quantitative assays
- Modular valve manifold supporting up to six-position, two-port switching—enabling column comparison, online sample cleanup (e.g., trap-and-elute), and orthogonal 2D-LC configurations
- Sealed high-pressure fluidic path with ceramic-coated pistons and chemically resistant seals—validated for continuous operation at 130 MPa with acetonitrile/water and common acidic/basic mobile phases
Sample Compatibility & Compliance
The EClassical 3200L accommodates standard 2.1–4.6 mm ID analytical columns, including reversed-phase C18, HILIC, ion-exchange, and chiral stationary phases. Its pressure and flow versatility supports method transfer from conventional HPLC to UHPLC-scale separations without hardware modification. The system complies with core instrumental requirements outlined in ASTM D7214 (liquid chromatographic system performance verification), ISO 17025 (for accredited testing laboratories), and USP (chromatography). Audit trail, electronic signature, and user-access-level controls are implemented in optional GLP/GMP-compliant software packages aligned with FDA 21 CFR Part 11 expectations.
Software & Data Management
Controlled via EliteChrom™ workstation software (Windows-based), the EClassical 3200L supports method building, sequence definition, real-time monitoring, and post-run processing—including peak integration using tangent skim, valley-to-valley, or manual baseline correction. Raw data files adhere to AIA/ANDI standards for long-term archival and third-party software interoperability (e.g., Chromeleon, OpenLab CDS). All instrument events—including pressure spikes, temperature deviations, and valve actuation—are timestamped and logged with operator ID. Optional modules include automated system suitability assessment (SSA) reporting per ICH Q2(R2) guidelines and customizable PDF report generation with embedded chromatograms and metadata.
Applications
- Rapid development and validation of stability-indicating methods for pharmaceutical small molecules and biologics
- Multi-residue pesticide screening in food matrices using online SPE-LC-MS/MS coupling interfaces
- Comprehensive profiling of natural product extracts via orthogonal column switching (e.g., C18 → phenyl-hexyl → amino)
- Quality control of polymer additives, surfactants, and industrial catalysts requiring high-resolution isocratic or gradient elution
- Environmental analysis of emerging contaminants (e.g., PFAS, microplastic leachates) with sub-ng/mL detection limits
FAQ
What column dimensions and particle sizes are supported at full 130 MPa pressure?
The system is validated for columns with internal diameters from 2.1 mm to 4.6 mm and particle sizes down to 1.7 µm, provided mobile phase viscosity and system dwell volume are accounted for during method translation.
Does the autosampler support 96-well or 384-well plate formats?
Yes—the standard tray accommodates two 96-well plates or one 384-well plate with programmable vial depth sensing and adaptive needle penetration control.
Is the UV-Vis detector compliant with pharmacopeial wavelength accuracy requirements?
Yes—wavelength verification is performed using holmium oxide and didymium filters per USP , with factory-certified accuracy of ±1 nm across the 190–800 nm range.
Can the column oven operate below ambient temperature?
Yes—the Peltier-based cooling module achieves stable operation at 5 °C even in laboratory environments up to 30 °C ambient.
What cybersecurity protocols are implemented in the embedded controller firmware?
The instrument’s ARM Cortex-based controller runs a hardened Linux kernel with disabled unused network services, TLS 1.2 encryption for remote diagnostics, and configurable password policies meeting NIST SP 800-63B Level 2 requirements.
