Elite EClassical3200 High-Performance Liquid Chromatography (HPLC) System
| Brand | Elite |
|---|---|
| Origin | Jiangsu, China |
| Manufacturer Type | Direct Manufacturer |
| Product Category | Domestic |
| Model | EClassical3200 |
| Price Range | USD 14,000 – 56,000 (FOB) |
| Instrument Type | Conventional High-Performance Liquid Chromatograph |
| Application Scope | General-purpose analytical use |
| Flow Rate Range | 0.001–10.000 mL/min |
| Maximum Operating Pressure | 130 MPa |
| Column Oven Temperature Control Range | 4–85 °C |
| UV-Vis Detector Wavelength Range | 190–800 nm |
Overview
The Elite EClassical3200 High-Performance Liquid Chromatography (HPLC) System is a fully integrated, domestically engineered benchtop chromatographic platform designed for routine and method-development applications in regulated and non-regulated laboratory environments. Built upon proven liquid chromatography principles—namely, separation of analytes based on differential partitioning between a mobile phase (liquid solvent gradient or isocratic) and a stationary phase (packed capillary or analytical column)—the EClassical3200 delivers consistent retention time reproducibility, baseline stability, and peak resolution across diverse sample matrices. Its architecture supports both reversed-phase and normal-phase separations, with compatibility for C18, phenyl-hexyl, HILIC, and ion-exchange columns. The system’s modular design enables flexible configuration: users may deploy it as a standalone isocratic or gradient-capable LC, or integrate it into larger automated workflows via industry-standard RS-232, USB, or optional Ethernet interfaces.
Key Features
- High-pressure binary gradient pump with dual-piston design, delivering flow accuracy ±0.1% RSD and pulseless delivery across the full 0.001–10.000 mL/min range
- Column compartment with precise Peltier-based temperature control (4–85 °C), ±0.2 °C stability, and real-time monitoring for method robustness
- Variable-wavelength UV-Vis detector featuring a deuterium/tungsten dual-lamp source, photodiode array option available, and wavelength accuracy ±1 nm (190–800 nm)
- Autosampler with 100-position vial capacity, injection volume programmable from 0.1 to 100 µL, and needle wash function to minimize carryover (<0.005% typical)
- Integrated system controller with embedded Linux OS, supporting method storage, sequence scheduling, and real-time pressure/flow/wavelength diagnostics
- Front-panel touchscreen interface (7-inch capacitive display) with intuitive icon-driven navigation and multilingual UI (English, Chinese, Spanish preloaded)
Sample Compatibility & Compliance
The EClassical3200 accommodates a broad spectrum of sample types—including small-molecule pharmaceuticals, natural product extracts, food additives, environmental contaminants (e.g., PAHs, pesticides), and polymer oligomers—provided they are soluble in common HPLC-grade solvents (acetonitrile, methanol, water, buffer systems). It meets mechanical and electrical safety requirements per GB/T 14710 (Chinese national standard for environmental adaptability of analytical instruments) and conforms to electromagnetic compatibility (EMC) standards GB/T 18268.1–2010. While not pre-certified for FDA 21 CFR Part 11, the system supports audit-trail-enabling software configurations and electronic signature readiness when paired with Elite’s optional ChromaLink LIMS-ready acquisition software. It is routinely deployed in laboratories adhering to ISO/IEC 17025, USP , and Chinese Pharmacopoeia general chapters for chromatographic analysis.
Software & Data Management
ChromaLink Lite—a Windows-based acquisition and processing suite—is supplied standard with the EClassical3200. It provides real-time chromatogram visualization, peak integration using tangent skim or valley-to-valley algorithms, calibration curve generation (linear, quadratic, weighted), and report export in PDF, CSV, and XML formats. For regulated environments, ChromaLink Pro adds role-based user management, electronic signatures, instrument qualification templates (IQ/OQ), and full audit trail logging compliant with ALCOA+ principles. All raw data files (.ecd) are stored in vendor-neutral, open-format structures to ensure long-term accessibility independent of proprietary software upgrades.
Applications
- Pharmaceutical QC/QA: Assay determination, related substances testing, dissolution profiling per USP/ChP monographs
- Academic research: Metabolite profiling, natural product isolation verification, polymer molecular weight distribution analysis
- Food & beverage: Mycotoxin quantification, vitamin content validation, artificial colorant screening
- Environmental testing: Detection of endocrine disruptors (e.g., bisphenol A), herbicide residues in soil/water extracts
- Chemical manufacturing: Reaction monitoring, purity assessment of intermediates and final APIs
FAQ
Is the EClassical3200 compatible with third-party CDS platforms such as Empower or Chromeleon?
Yes—via ASCII-based ASCII/CSV export and optional OPC UA server module, enabling bidirectional communication with major chromatography data systems.
What column dimensions and internal diameters does the system support natively?
Standard support includes 2.1–4.6 mm ID columns up to 250 mm length; guard column holder and zero-dead-volume fittings included.
Does the autosampler include cooling capability for temperature-sensitive samples?
No built-in refrigeration; ambient operation only. For thermolabile samples, users may integrate external chillers or use pre-chilled vial racks.
Can the system be upgraded to UHPLC performance levels?
Not retrofittable—maximum pressure rating is fixed at 130 MPa, optimized for HPLC-grade columns (≤5 µm particle size); sub-2 µm columns require dedicated UHPLC instrumentation.
What service and calibration documentation is provided upon installation?
Factory calibration certificate, IQ/OQ protocol templates, preventive maintenance checklist, and traceable pressure/flow/wavelength verification records are delivered digitally and in hard copy.
