Eppendorf Research® plus Single-Channel Adjustable-Volume Pipette, 0.5–5 mL

Overview

The Eppendorf Research® plus Single-Channel Adjustable-Volume Pipette (0.5–5 mL) is a precision-engineered manual air-displacement pipette designed for high-reproducibility liquid handling in regulated and research-intensive laboratory environments. Based on the proven piston-in-cylinder principle, it delivers consistent volumetric accuracy across its full range—leveraging temperature-stable materials, low-friction sealing components, and a calibrated spring mechanism to minimize operator-induced variability. Unlike fixed-volume devices, this pipette supports continuous volume adjustment within its specified range, enabling flexible experimental workflows without instrument switching. Its design adheres strictly to ISO 8655-2 (volumetric performance) and ISO 8655-5 (user-related requirements), ensuring traceable, auditable performance under GLP and GMP frameworks.

Key Features

• PhysioCare Concept integration: Ultra-lightweight construction (≈115 g) reduces hand and forearm muscle load during repetitive operations—validated by ergonomic studies on grip force reduction and wrist joint angle optimization.
• Elastic tip cone technology: Enables effortless tip attachment and ejection across all volumes up to 1 mL; for the 0.5–5 mL model, this translates into significantly reduced thumb force during tip loading—critical for extended shift work or users with repetitive strain concerns.
• One-touch volume reset: Restores factory calibration settings without tools or external recalibration—ideal for shared instruments or multi-user labs where procedural consistency must be preserved between shifts.
• Full or partial autoclaving: The entire pipette can undergo steam sterilization at 121 °C, 1 bar for 20 minutes; alternatively, only the lower assembly (tip cone, sleeve, and O-rings) may be autoclaved—preserving electronic-free reliability while maintaining sterility assurance for cell culture or molecular biology applications.
• Chemical resistance: Housing and internal components withstand exposure to common solvents including 70% ethanol, isopropanol, and dilute acids/bases—supporting decontamination protocols without dimensional drift or seal degradation.
• Purple color coding: Complies with international pipette volume identification standards (ISO 8655 Annex B), facilitating rapid visual verification and minimizing cross-volume usage errors.

Sample Compatibility & Compliance

This pipette is validated for aqueous, viscous, and moderately volatile liquids—including PBS, cell culture media, DNA/RNA solutions, and ethanol-based reagents. Performance stability has been verified at altitudes up to 3,000 m above sea level via pressure-compensated piston travel calibration. It meets ISO 8655-2 accuracy tolerances (±0.8% at nominal volume) and precision specifications (CV ≤ 0.3%). Documentation packages—including Certificate of Conformance, traceable calibration reports, and maintenance logs—are available to support FDA 21 CFR Part 11–aligned electronic record systems when paired with Eppendorf’s optional data capture accessories.

Software & Data Management

As a fully mechanical, non-electronic device, the Research® plus 0.5–5 mL pipette requires no firmware, battery, or software interface. However, its standardized output format enables seamless integration into digital lab notebooks (e.g., LabArchives, Benchling) via manual entry or barcode-scanned lot tracking (using supplied serial-numbered labels). For labs implementing instrument lifecycle management, Eppendorf provides downloadable Excel-based calibration log templates compliant with ISO/IEC 17025 and internal audit requirements.

Applications

• Preparation of standard curves and dilution series in ELISA, qPCR, and immunoassay workflows
• Transfer of serum, plasma, or tissue lysates in clinical diagnostics and biobanking
• Media and supplement dispensing in mammalian and insect cell culture facilities
• Reagent addition in high-throughput screening (HTS) plate setups where manual flexibility outweighs automation ROI
• Teaching laboratories requiring robust, serviceable instruments with clear metrological traceability

FAQ

Can this pipette be autoclaved with the upper body intact?
Yes—the entire unit is rated for full-cycle autoclaving at 121 °C, 1 bar, 20 minutes. However, repeated full autoclaving may accelerate wear on the volume adjustment mechanism; Eppendorf recommends partial autoclaving (lower assembly only) for routine use.
Is tip ejection force consistent across the full 0.5–5 mL range?
Tip ejection force remains optimized for human ergonomics up to 1 mL; above that, ejection relies on standard mechanical leverage—still well below ISO-defined maximum thresholds (≤15 N), but not enhanced by the elastic cone feature.
Does the pipette require recalibration after autoclaving?
No—its mechanical design maintains dimensional stability post-sterilization. The one-touch reset function restores nominal volume settings instantly, eliminating need for external recalibration unless physical damage occurs.
What documentation is provided for quality assurance compliance?
A Certificate of Conformance is included. Optional ISO/IEC 17025-accredited calibration certificates—with uncertainty budgets and traceability to PTB/NIST standards—are available upon request.
How does altitude affect performance—and how is compensation achieved?
Air displacement pipettes are inherently sensitive to ambient pressure changes. The Research® plus incorporates a pressure-balanced piston chamber and adjustable spring preload, allowing users to perform rapid, tool-free volume offset correction (typically ±0.2–0.5% per 1,000 m elevation gain) directly at the bench.