Fuli LC5190 Low-Pressure Ultra-High Performance Liquid Chromatograph

Overview

The Fuli LC5190 Low-Pressure Ultra-High Performance Liquid Chromatograph is an engineered solution for laboratories requiring HPLC-grade robustness with UHPLC-level separation efficiency—without demanding ultra-high backpressure operation. Based on Couette-flow-compatible pump architecture and optimized fluidic pathway design, the LC5190 operates reliably within conventional HPLC pressure limits (≤60 MPa), yet delivers resolution and sensitivity comparable to systems designed for sub-2-µm particle columns. Its modular architecture supports seamless integration of 1.8 µm, 2.7 µm, 3.5 µm, and 5 µm packed columns—enabling direct method transfer from legacy HPLC protocols without revalidation. The system implements a dual-mode solvent delivery strategy: configurable as isocratic, binary high-pressure, quaternary low-pressure, or dual-binary gradient systems—each maintaining flow accuracy ≤0.1% RSD across full pressure and flow ranges. All pumps feature in-line degassing modules, automatic internal pressure calibration, and MS-compatible pulse-dampened flow profiles.

Key Features

• Multi-configuration solvent delivery: Selectable isocratic, binary high-pressure, quaternary low-pressure, or dual-binary gradient modes—all with real-time flow/pressure feedback and auto-compensation.
• Zero-loss high-pressure injection: Achieves ≤0.01% carryover via pressurized needle wash, independent degassing of wash solvents, and 0.1 µL volumetric precision; supports pre-load injection with ≤5 s cycle time.
• UV-Vis detector with 190–900 nm spectral coverage: Equipped with transmission-mode deuterium and tungsten lamps, built-in mercury lamp wavelength validation, and face-forward optical path design for simplified lamp/detector cell maintenance.
• Thermostatted column compartment with active air-circulation cooling/heating (5–85 °C), e-record-enabled smart column tracking, zero-dead-volume fingertight fittings, and integrated leak-sensing via gas-sensitive semiconductor sensors.
• Native support for extended detection modalities: Compatible with diode array (DAD), fluorescence (FL), evaporative light scattering (ELSD), and refractive index (RI) detectors—enabling method flexibility across pharmaceutical QC, polymer characterization (GPC), amino acid analysis, and 2D-LC workflows.

Sample Compatibility & Compliance

The LC5190 accommodates standard 4.6 mm × 150 mm analytical columns as well as narrow-bore (2.1 mm i.d.) and microbore formats. Its pressure-tolerant flow path accepts silica-based, polymer-based, and hybrid stationary phases—including C18, phenyl-hexyl, HILIC, and SEC media. System compliance aligns with ISO/IEC 17025:2017 requirements for testing laboratories and supports GLP/GMP data integrity through audit-trail-enabled software (21 CFR Part 11 compliant configuration available). All hardware components undergo accelerated life-cycle testing per ASTM E2500-13 for instrument qualification, including pump endurance (>10,000 h MTBF), injector precision verification (per USP ), and detector linearity assessment (ASTM E1657-21).

Software & Data Management

Fuli’s native ChromSight™ control software provides intuitive sequence setup, real-time chromatogram visualization, peak integration with customizable baseline algorithms, and automated report generation in PDF/A-1a format. Raw data files are stored in vendor-neutral .cdf (NetCDF-based) format with embedded metadata (instrument parameters, user ID, timestamp, method version). Audit trails record all user actions—including method edits, sequence modifications, and result reprocessing—with immutable timestamps and digital signature options. Data export supports CSV, ASCII, and AIA/ANDI standards for third-party processing tools (e.g., Empower, Chromeleon, OpenLab CDS).

Applications

• Pharmaceutical quality control: Assay, related substances, residual solvents per ICH Q2(R2) guidelines.
• Biopolymer characterization: Molecular weight distribution analysis via GPC/SEC using low-dispersivity flow paths.
• Amino acid profiling: Post-column derivatization compatibility and low-background UV detection at 338 nm and 440 nm.
• Two-dimensional liquid chromatography (2D-LC): Integrated valve control for comprehensive heart-cutting and comprehensive modulation setups.
• Environmental analysis: Pesticide residue screening (EPA Method 8081B, 8321B) and PFAS quantification using high-sensitivity UV and tandem ELSD detection.

FAQ

Does the LC5190 support 21 CFR Part 11-compliant electronic records and signatures?
Yes—when configured with ChromSight™ Secure Mode, the system provides role-based access control, electronic signatures with biometric or token authentication, and full audit trail functionality meeting FDA 21 CFR Part 11 and EU Annex 11 requirements.
Can the LC5190 operate with 1.8 µm particle columns without exceeding 40 MPa backpressure?
Yes—the low-pressure UHPLC architecture reduces system dwell volume and minimizes extra-column band broadening, enabling stable operation with 1.8 µm columns at flow rates up to 0.8 mL/min while maintaining backpressure below 35 MPa under typical mobile phase conditions.
Is column oven cooling capability verified to ISO 17025 temperature uniformity standards?
Yes—the air-circulation column compartment achieves ±0.3 °C temperature stability and ±0.5 °C spatial uniformity across the full 5–85 °C range, validated per ISO/IEC 17025 Clause 6.4.3 for thermal management systems.
What detector interfaces are supported for third-party mass spectrometers?
The LC5190 features analog voltage output (0–1 V), RS-232/485 serial control, and optional Ethernet-based API integration for synchronization with Thermo, Sciex, Waters, and Agilent MS platforms.
How is solvent consumption minimized during method development?
The quaternary low-pressure mixing module enables precise gradient formation with <500 µL total system volume, reducing solvent waste by ~40% compared to traditional high-pressure mixing systems—verified per ASTM D7091-22 for green analytical chemistry metrics.