GelMaster-3000 Automated GPC Gel Permeation Chromatography System
| Brand | KEZHE SHANGHAI |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Model | GelMaster-3000 |
| Flow Rate | 0–10 mL/min |
| Max Pressure | 1 psi |
| Flow Stability | ±0.5% RSD |
| Auto-sampler Volume | 5 mL (standard loop, customizable) |
| Auto-sampler Capacity | 160 × 20 mL tubes (standard) |
| UV Detection Range | 190–800 nm |
| Column Dimensions | 20 × 300 mm |
| Packing Material | Bio-Beads S-X3 (200–400 mesh) |
| Solvent System | Ethyl Acetate/Cyclohexane (3:7 v/v) |
| Fraction Collector Options | 20 mL (160-position), 80 mL (40-position), 150 mL (12-position), 250–300 mL (8-position) |
| Solvent Recovery Valve | Integrated three-way valve for solvent recycling |
Overview
The GelMaster-3000 Automated GPC System is a high-precision, benchtop gel permeation chromatography (GPC) platform engineered for automated matrix cleanup and molecular weight-based fractionation of complex biological and environmental samples. Based on size-exclusion separation principles, the system utilizes calibrated porous polymer columns (e.g., Bio-Beads S-X3) to selectively retain low-molecular-weight analytes while eluting high-molecular-weight interferences—such as proteins, lipids, pigments, and polymeric residues—first. This orthogonal purification strategy is critical prior to downstream analysis by GC-MS, LC-MS/MS, or HPLC-UV, especially in regulated laboratories performing residue analysis under AOAC, EPA, FDA, and EU EN method frameworks. Designed for unattended operation over extended cycles, the GelMaster-3000 integrates robust fluidic architecture—including dual-piston preparative pumps, high-reproducibility syringe-based autosampling, and full-spectrum UV-Vis detection—to deliver consistent, trace-level recovery of target compounds from food, feed, pharmaceutical, and environmental matrices.
Key Features
- Dual-piston high-pressure preparative pump delivering stable flow across 0–10 mL/min with ≤0.5% RSD at 1 mL/min, ensuring retention time reproducibility across multi-hour runs.
- Automated liquid handler with precision syringe-driven sampling; standard 5 mL loop (customizable to 1–20 mL), enabling repeatable injection volumes without carryover or dilution artifacts.
- Full-wavelength UV-Vis detector (190–800 nm) equipped with deuterium-tungsten lamp combination, supporting simultaneous monitoring at multiple wavelengths for co-eluting compound discrimination.
- Stainless-steel GPC columns (20 × 300 mm) packed with Bio-Beads S-X3 (200–400 mesh), validated for use with EPA Method 3640A and AOAC 984.21 solvent systems (ethyl acetate/cyclohexane, 3:7 v/v).
- Modular fraction collector with configurable tray options: 160-position 20 mL tube rack (standard), 40-position 80 mL tubes, 12-position 150 mL concentrator cups, and 8-position 250–300 mL vessels—compatible with parallel evaporation and SPE platforms.
- Integrated solvent recovery three-way valve, diverting clean eluent back to solvent reservoirs to reduce consumables cost and waste generation per run.
- Self-contained control interface with fully localized English-language software; all modules—including pump, detector, autosampler, and fraction collector—are synchronized via deterministic timing protocols compliant with GLP audit trails.
Sample Compatibility & Compliance
The GelMaster-3000 is validated for extraction and cleanup of analytes from diverse sample types including animal tissues, dairy products, cereals, soil extracts, and biopharmaceutical formulations. It meets performance criteria outlined in U.S. EPA SW-846 Method 3640A (gel permeation chromatography), AOAC Official Method 984.21 (pesticide residue cleanup), FDA Guidance Document 2905A (veterinary drug residue analysis), and European Standards EN 12393 (pesticides in animal fat) and EN 1528 (PAHs in food). All hardware components comply with IEC 61010-1 safety requirements for laboratory equipment. Data acquisition and instrument control support 21 CFR Part 11–compliant electronic signatures and audit-ready event logging when paired with optional LIMS-integrated software modules.
Software & Data Management
The embedded control software provides real-time chromatogram visualization, peak integration, fraction trigger logic (time-, volume-, or threshold-based), and method sequencing for batch processing. Raw data files are saved in vendor-neutral .csv and .dx formats, compatible with third-party chemometric tools (e.g., Chromeleon, OpenLab CDS). Audit trail functionality records user actions, parameter changes, and system events with timestamps and operator IDs. Optional software upgrades enable remote monitoring via secure HTTPS, scheduled maintenance alerts, and automated report generation aligned with ISO/IEC 17025 documentation templates.
Applications
- Cleanup of pesticide, veterinary drug, and mycotoxin residues from lipid-rich food matrices prior to GC-MS quantification.
- Removal of immunoglobulins, albumin, and polysaccharides from monoclonal antibody and recombinant protein preparations during QC release testing.
- Isolation of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) from sediment and sludge extracts per EPA 8270D workflows.
- Purification of vaccine antigens (e.g., recombinant hepatitis B surface antigen) and antitoxins (e.g., tetanus, botulinum, snake venoms) from fermentation broths.
- Preparative fractionation of growth factors (e.g., rhEGF, rhbFGF) and cytokines (e.g., IL-2, IFN-γ) for stability and potency assays in biologics development.
FAQ
What column chemistries are supported beyond Bio-Beads S-X3?
The system accepts standard 20 mm ID × 300 mm GPC columns packed with cross-linked polystyrene-divinylbenzene (PS-DVB) or silica-based SEC media, provided they are rated for ≤1 psi operating pressure and compatible with organic mobile phases.
Can the GelMaster-3000 be integrated into an existing LIMS environment?
Yes—via optional OPC UA or REST API interface modules, enabling bidirectional transfer of method parameters, run logs, and fraction metadata.
Is column regeneration supported under standard operating procedures?
Bio-Beads S-X3 columns may be regenerated using sequential rinses with methanol, chloroform, and hexane per manufacturer guidelines; column lifetime is typically ≥200 injections under validated conditions.
Does the system meet requirements for GLP-compliant laboratories?
When configured with audit trail-enabled software and electronic signature modules, the GelMaster-3000 satisfies core GLP elements including data integrity, operator accountability, and method version control per OECD Principles of Good Laboratory Practice.
What maintenance intervals are recommended for the syringe-based autosampler?
Syringe seals and needle seats should be inspected every 500 injections; full calibration verification is advised quarterly or after any hardware service event.
