Hanbon NS8030A Preparative Supercritical Fluid Chromatography System
| Brand | Hanbon |
|---|---|
| Origin | Jiangsu, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Origin | Domestic (China) |
| Model | NS8030A |
| Instrument Type | Preparative |
| Detector Type | UV |
| Maximum System Pressure | 20 MPa |
| Flow Rate Range | 0–300 mL/min |
| CO₂ Recovery System | Integrated |
| Collection Technology | Patented Cyclonic Collector |
Overview
The Hanbon NS8030A Preparative Supercritical Fluid Chromatography (SFC) System is an engineered platform for high-efficiency chiral and achiral compound purification at milligram-to-gram scale. It utilizes supercritical carbon dioxide (scCO₂), optionally modified with organic co-solvents (e.g., methanol, ethanol, isopropanol), as the primary mobile phase. Under precisely controlled temperature (typically 35–65 °C) and backpressure (up to 20 MPa), scCO₂ exhibits tunable solvent strength, low viscosity, and high diffusivity—enabling rapid mass transfer, sharp peak resolution, and reduced analysis time compared to conventional liquid chromatography. The system operates on a closed-loop fluid management architecture: CO₂ exiting the column is depressurized, filtered, cooled, re-liquefied, and reintroduced into the supply circuit—minimizing consumable usage and operational cost while supporting environmentally responsible laboratory practices.
Key Features
- Robust high-pressure fluid delivery: Dual-piston, pulse-dampened CO₂ pump with integrated co-solvent gradient capability, delivering stable flow across 0–300 mL/min with ≤0.5% RSD.
- Intelligent backpressure regulation: Digital electronic pressure control (EPC) maintains setpoint accuracy within ±0.1 MPa over extended runs, critical for retention time reproducibility and method transferability.
- UV detection with variable wavelength: Fixed or programmable dual-wavelength UV/Vis detector (190–400 nm) optimized for real-time fraction triggering and purity assessment during collection.
- Proprietary cyclonic fraction collector: Engineered for continuous-phase separation of scCO₂ vapor from condensed fractions; achieves >92% CO₂ recovery efficiency and eliminates solvent carryover in collected analytes.
- Modular thermal management: Independently controlled column oven (ambient to 80 °C, ±0.2 °C stability) and post-column heat exchanger ensure consistent phase behavior and detector baseline stability.
- Full-system integration: All modules—including pump, modifier pump, oven, detector, collector, and backpressure regulator—are synchronized via embedded real-time OS and Ethernet-based communication protocol.
Sample Compatibility & Compliance
The NS8030A accommodates a broad range of thermally labile, non-volatile, and chiral compounds—including pharmaceutical intermediates, natural product isolates, synthetic polymers, and lipid derivatives—without derivatization. Its low-temperature, low-residue elution profile preserves molecular integrity and simplifies downstream handling. The system supports method development compliant with ICH Q5A, Q5C, and USP <621> guidelines for chiral separations. Data acquisition and instrument control software meet ALM (Audit Log Management) requirements per FDA 21 CFR Part 11 when deployed with validated user access controls and electronic signature modules. Routine operation aligns with ISO/IEC 17025 quality management principles for testing laboratories.
Software & Data Management
Hanbon SFC Control Suite v3.2 provides intuitive method setup, real-time chromatogram visualization, automated fraction collection logic (threshold-based, time-windowed, or peak-area-triggered), and integrated calibration tracking. Raw data files are stored in vendor-neutral .cdf format (compatible with third-party processing tools such as Chromeleon, Empower, and OpenChrom). Audit trails record all parameter changes, user logins, and sequence executions with timestamp, operator ID, and reason-for-change fields. Export options include CSV, PDF reports, and structured XML for LIMS integration. Remote monitoring via secure HTTPS interface enables off-site system status checks and method queue management.
Applications
- Chiral purification of active pharmaceutical ingredients (APIs) and racemic mixtures under cGMP-aligned workflows.
- Isolation of bioactive constituents from plant extracts and fermentation broths where thermal degradation limits HPLC applicability.
- Preparative separation of synthetic oligomers and functionalized small molecules for structure–activity relationship (SAR) studies.
- Method scouting and scale-up support—from analytical screening (using 2–4.6 mm ID columns) to semi-preparative (10–30 mm ID) and preparative (50 mm ID) formats.
- Green chemistry process development: Replacement of chlorinated or high-boiling solvents (e.g., chloroform, DMSO) with recyclable CO₂-based mobile phases.
FAQ
What is the maximum allowable operating pressure for the NS8030A system?
The system is rated for continuous operation up to 20 MPa (2900 psi), with safety interlocks preventing exceedance of this limit.
Can the NS8030A be used for analytical-scale separations?
Yes—by configuring low-flow pumps, narrow-bore columns, and micro-UV flow cells, the platform supports analytical method development prior to scale-up.
Is CO₂ recycling mandatory, or can the system operate in open-mode?
The CO₂ recovery loop is standard and integral to system design; open-mode operation is not supported due to regulatory, economic, and environmental considerations.
Does the system support multi-solvent modifier gradients?
It supports binary gradient mixing (CO₂ + one organic modifier); tertiary or quaternary gradients require external blending modules and are not natively integrated.
How is fraction purity verified during collection?
Real-time UV absorbance profiles are monitored; optional integration with inline FTIR or MS detectors enables orthogonal confirmation of eluent composition and identity.
