Hanuo HN70-4A Microplate Incubator Shaker
| Brand | Hanuo |
|---|---|
| Origin | Shanghai, China |
| Model | HN70-4A |
| Instrument Type | Full-temperature microplate shaker |
| Timer Range | 0–99 h 59 min |
| Oscillation Speed | 300–1200 rpm |
| Orbit Diameter | 3 mm |
| Temperature Range | Ambient +5°C to 70°C |
| Plate Capacity | 4 standard microplates (96-well or 384-well) |
| Temperature Uniformity | ≤ ±0.5°C |
| Temperature Stability | ≤ ±0.2°C |
| Heating Accuracy | < ±0.3°C at 37°C |
| Ramp Time | < 25 min (20°C → 70°C) |
| Drive Motor | Brushless DC motor |
| Power Consumption | 250 W |
| Dimensions (W×D×H) | 345 × 310 × 178 mm |
Overview
The Hanuo HN70-4A Microplate Incubator Shaker is an integrated thermal and mechanical platform engineered for precise incubation and homogeneous mixing of biological samples in standard microplates. Designed around a PID-controlled dual-zone heating system and a brushless DC motor-driven horizontal orbital shaking mechanism, it delivers simultaneous temperature regulation and consistent agitation—critical for applications including enzyme-linked immunosorbent assay (ELISA) development, cell-based assays, kinetic binding studies, and microbial suspension culture. Its compact footprint, low acoustic emission (<50 dB(A)), and uniform thermal distribution across all plate positions support reproducible experimental conditions in regulated laboratory environments.
Key Features
- Four independent microplate positions accommodating up to four 96-well or 384-well plates simultaneously—optimized for high-throughput assay workflows.
- Full-range temperature control from ambient +5°C to 70°C with stability ≤ ±0.2°C and uniformity ≤ ±0.5°C across the entire heating surface; validated accuracy < ±0.3°C at 37°C per internal calibration protocol.
- Horizontal orbital shaking with fixed 3 mm orbit diameter and speed range 300–1200 rpm, ensuring gentle yet effective mixing without splashing or edge effects in low-volume wells.
- Intuitive LCD interface with real-time display of setpoint/actual temperature, elapsed time, remaining timer duration, and current oscillation speed—enabling full parameter visibility during operation.
- Touch-sensitive control panel with ergonomic layout, seamless stainless-steel heating deck, and smooth polymer housing designed for easy decontamination and compliance with biosafety cleaning protocols.
- Auto-restart function after power interruption preserves experimental integrity by resuming pre-set parameters—including temperature, speed, and remaining time—without manual reconfiguration.
- Brushless DC motor drive ensures maintenance-free operation over extended service life (>20,000 hours MTBF), eliminating carbon brush wear and minimizing torque fluctuation across speed ranges.
Sample Compatibility & Compliance
The HN70-4A supports all ANSI/SBS-compliant microplates (including PCR, ELISA, cell culture, and deep-well formats) and accommodates standard accessories such as plate seals, humidity domes, and CO2-permeable membranes. Its thermal and mechanical performance meets requirements for GLP-aligned workflows and aligns with key method validation principles outlined in ISO/IEC 17025:2017 (clause 7.2.2 on equipment verification). While not certified for IVD or clinical diagnostic use, its temperature traceability and repeatability support data integrity under FDA 21 CFR Part 11 when paired with compliant electronic lab notebook (ELN) systems.
Software & Data Management
The device operates autonomously via embedded firmware with no external software dependency. All operational parameters are stored in non-volatile memory and retain configuration through power cycles. For integration into automated labs, optional RS-232 or USB-to-serial interfaces (available upon request) enable remote command execution and status polling using ASCII-based protocols compatible with LabVIEW, Python pySerial, or custom LIMS middleware. Audit trails—including start/stop timestamps, temperature logs at 10-second intervals, and speed deviation alerts—are exportable in CSV format for QA review and regulatory submission preparation.
Applications
- ELISA and immunoassay plate incubation with concurrent shaking to enhance antigen-antibody binding kinetics.
- Cell-based assays requiring synchronized temperature control and gentle agitation to maintain monolayer integrity during staining or transfection steps.
- Molecular biology workflows involving hybridization, probe annealing, or enzymatic reactions in multiwell formats.
- Microbial suspension culture for OD monitoring, antibiotic susceptibility testing (AST), and growth curve analysis.
- High-content screening (HCS) plate preconditioning prior to imaging or cytometry readouts.
- Quality control of reagent stability under accelerated thermal stress conditions (e.g., 40°C/75% RH).
FAQ
Is the HN70-4A suitable for use with volatile organic solvents in microplates?
No. The unit is designed for aqueous and buffered biological solutions only. Solvent exposure may compromise gasket integrity and sensor calibration.
Can I operate two different temperature zones independently on this model?
No. The HN70-4A features a single, uniformly heated deck surface. Temperature gradients across the plate array are minimized but not individually addressable.
What is the maximum recommended fill volume per well during shaking at 1200 rpm?
For 96-well plates, ≤ 200 µL per well is advised to prevent cross-contamination and ensure orbital consistency. Lower volumes (≤ 100 µL) are recommended above 900 rpm.
Does the device include built-in temperature calibration functionality?
Yes. A two-point calibration mode (at 37°C and 60°C) is accessible via service menu for field adjustment against NIST-traceable reference thermometers.
Is CE or UL certification available for this instrument?
The HN70-4A complies with IEC 61010-1:2010 for laboratory electrical safety and EMC (EN 61326-1:2013). CE marking is provided for EU export configurations; UL listing requires regional variant assembly and additional third-party testing.
