Hanuo HNMTH-100 Heated Vortex Mixer with Temperature Control

Overview

The Hanuo HNMTH-100 Heated Vortex Mixer with Temperature Control is an integrated thermo-shaking platform engineered for precise thermal management and reproducible mechanical agitation in molecular biology, clinical diagnostics, and pharmaceutical R&D workflows. Unlike conventional water baths or standalone shakers, this instrument combines Peltier-free resistive heating, microprocessor-regulated orbital oscillation (3 mm horizontal rotation), and programmable timing into a single compact benchtop unit. Its operational principle relies on closed-loop PID temperature control coupled with brushless DC motor-driven motion—ensuring minimal thermal gradient across the sample block and high repeatability of mixing kinetics. The device is optimized for applications requiring simultaneous incubation and homogenization of low-volume samples in PCR tubes, 96-well plates, deep-well plates, and microcentrifuge tubes—particularly where thermal consistency and shear-sensitive sample integrity are critical.

Key Features

• Single-platform design with quick-swap modular blocks compatible with standard tube formats (0.2 mL, 0.5 mL, 1.5 mL, 2.0 mL) and microplates (96-well, 384-well, deep-well)
• Precision temperature control from ambient +5°C to 100°C with ±0.3°C stability and ≤±0.3°C uniformity across the entire block surface
• Adjustable oscillation frequency spanning 300–2000 rpm, enabling optimization for both gentle resuspension and vigorous mixing protocols
• Programmable timer with 1-second resolution and up to 100-hour duration; supports countdown, auto-shutdown, and resume-on-power-restoration functionality
• Integrated temperature calibration routine accessible via front-panel interface for traceable adjustment per laboratory SOP requirements
• Short-pulse “touch-mix” mode for rapid manual agitation without altering active program settings
• Brushless DC motor architecture delivering maintenance-free operation over >20,000 hours MTBF and silent, vibration-damped performance
• Dual-level safety system including over-temperature cutoff, thermal runaway detection, and hardware-based current limiting

Sample Compatibility & Compliance

The HNMTH-100 accommodates all common life science consumables—including thin-walled PCR tubes, skirted and non-skirted microplates, conical-bottom tubes, and filter plates—without adapter modification. Its flat aluminum heating block ensures uniform thermal transfer and minimizes condensation risk during extended incubations. From a regulatory standpoint, the instrument meets essential electrical safety requirements per IEC 61010-1 and is designed to support compliance with Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) environments. While not FDA 21 CFR Part 11–certified out-of-the-box, its deterministic control logic, non-volatile parameter storage, and timestamped event logging (via optional RS232/USB data export) facilitate integration into validated workflows aligned with ISO/IEC 17025, USP , and ASTM E2500 standards.

Software & Data Management

The HNMTH-100 operates via an intuitive membrane keypad and dual-line LCD display, eliminating dependency on external software for routine use. All parameters—including setpoint temperature, oscillation speed, timer value, and calibration offset—are stored in non-volatile memory and retained through power cycles. For labs requiring electronic recordkeeping, optional serial communication (RS232 or USB virtual COM port) enables connection to LIMS or ELN systems for automated parameter upload/download and real-time status polling. Firmware supports firmware version reporting and user-accessible diagnostic codes for preventive maintenance scheduling. No proprietary drivers or cloud services are required—data exchange follows ASCII-based command-response protocol compliant with ANSI X3.4-1986.

Applications

• Enzyme reaction setup (restriction digests, ligations, phosphorylations) requiring precise temperature synchronization with mixing
• Cell lysis and protein extraction protocols where mechanical shear must be balanced with thermal denaturation control
• qPCR and RT-qPCR master mix preparation with uniform reagent resuspension prior to thermal cycling
• Antibody labeling and conjugation reactions sensitive to localized overheating or sedimentation
• High-throughput assay plate conditioning (e.g., ELISA pre-incubation, bead-based immunoassays)
• Controlled solubilization of lyophilized reagents or cryopreserved cell pellets
• Quality control testing of temperature-dependent reagent stability under dynamic agitation conditions

FAQ

Does the HNMTH-100 support cooling functionality?
No—the HNMTH-100 is a heated-only model. For combined heating/cooling capability, refer to the HNMTC-100 series.
Can multiple temperature zones be controlled independently?
No—it features a single-zone heating block. Multi-zone control requires modular incubator-shaker systems with separate thermal domains.
Is validation documentation (IQ/OQ/PQ) available?
Standard factory test reports are included. Custom IQ/OQ protocols and PQ templates can be supplied upon request for GxP-regulated installations.
What is the maximum load capacity for the standard block?
The platform supports up to 1.2 kg distributed mass; exceeding this may affect thermal uniformity and motor longevity.
Are third-party blocks supported?
Yes—blocks conforming to DIN 12880 dimensions and featuring M4 threaded mounting holes are mechanically and thermally compatible.