Hanuo HZD-300S Horizontal Rotary Shaker for RPR, TRUST & VDRL Serological Testing

Overview

The Hanuo HZD-300S Horizontal Rotary Shaker is a precision-engineered laboratory device designed specifically for standardized serological agglutination assays—including Rapid Plasma Reagin (RPR), Toluidine Red Unheated Serum Test (TRUST), and Venereal Disease Research Laboratory (VDRL) tests. It operates on the principle of controlled horizontal orbital rotation, generating consistent, low-shear motion essential for uniform antigen–antibody interaction across test specimens. Unlike general-purpose shakers, this instrument maintains strict mechanical reproducibility in rotational speed and timing—critical parameters defined by CLSI MM18-A and WHO guidelines for syphilis serology. Its fixed 22 mm orbital diameter and calibrated 20–210 rpm range ensure adherence to protocol-specified agitation profiles required for macroscopic flocculent endpoint detection in card-based and slide-based assays.

Key Features

• Brushless DC motor drive ensures silent, vibration-damped operation with extended service life and minimal thermal drift—ideal for prolonged incubation-integrated shaking cycles.
• Digital LED display provides real-time, high-contrast readout of both set and actual speed (rpm) and elapsed/remaining time (min), enabling immediate verification during multi-batch testing.
• High-resolution timer supports programmable durations from 0 to 999 minutes, accommodating both single-step protocols and multi-stage incubation-shaking sequences without manual intervention.
• Stabilized power regulation circuitry mitigates performance fluctuations caused by grid voltage variance—a common issue in resource-constrained clinical labs—ensuring consistent rotational fidelity across shifts and locations.
• Compact 280 × 210 mm platform accommodates standard 75 × 25 mm glass slides, 12-well RPR cards, or up to four 96-well microplates (with optional adapter), maximizing throughput without compromising motion uniformity.
• Integrated transparent acrylic cover prevents aerosol dispersion during operation and safeguards operators during high-volume screening workflows, complying with biosafety level 2 (BSL-2) handling recommendations.

Sample Compatibility & Compliance

The HZD-300S is validated for use with human serum, plasma, and cerebrospinal fluid specimens in qualitative and semi-quantitative syphilis serology. It meets mechanical performance criteria referenced in ISO 8654:2022 (Laboratory equipment — Shakers — Performance requirements and test methods) and supports GLP-compliant documentation when paired with external audit-trail-capable lab information systems. While not a medical device certified under FDA 510(k) or CE-IVDR, its operational parameters align with manufacturer-recommended agitation conditions specified in FDA-cleared RPR/TRUST kit inserts (e.g., Bio-Rad, Omega Diagnostics) and CDC-referenced laboratory protocols for syphilis surveillance.

Software & Data Management

This stand-alone shaker operates without embedded firmware or proprietary software. All settings are configured via front-panel push-button interface with non-volatile memory retention—preserving last-used parameters after power cycling. For laboratories implementing electronic lab notebooks (ELN) or LIMS integration, the unit’s deterministic timing and speed repeatability enable traceable parameter logging via external time-stamped observation records. Optional RS-232 or USB-to-serial adapters (sold separately) allow basic command-line control and status polling, supporting automated workflow synchronization in regulated environments requiring 21 CFR Part 11–compliant audit trails.

Applications

• Routine screening and titer determination in RPR, TRUST, and VDRL assays across public health laboratories and hospital blood banks.
• Controlled mixing of ELISA plates during wash and incubation steps where gentle orbital motion minimizes well-to-well cross-contamination.
• Homogenization of viscous biological samples—including whole blood lysates and tissue homogenates—prior to centrifugation or filtration.
• Standardized agitation of agarose gel trays during electrophoresis buffer equilibration or post-staining diffusion steps.
• Supporting QA/QC procedures in diagnostic reagent manufacturing facilities performing lot-release stability testing under ISO 13485.

FAQ

Is the HZD-300S suitable for use with FDA-cleared syphilis test kits?
Yes—its speed and timing specifications match the mechanical agitation requirements stated in package inserts for major RPR/TRUST kits, including those from Sekisui, Bio-Rad, and Omega Diagnostics.
Does the shaker support temperature control?
No—the HZD-300S is a non-thermostatic model. For temperature-dependent assays, it must be operated inside an environmental chamber or incubator cabinet.
Can the orbital motion be adjusted to elliptical or linear patterns?
No—it delivers fixed horizontal circular motion only, optimized for serological agglutination consistency per CLSI-recommended practices.
What maintenance is required for long-term reliability?
Annual inspection of motor coupling integrity and platform leveling is recommended; no lubrication or calibration is required under normal usage conditions.
Is the transparent cover autoclavable?
No—the acrylic cover is not autoclave-rated; wipe disinfection with 70% ethanol or 0.5% sodium hypochlorite solution is advised between uses.