Hanuo SHA-BA Water Bath Constant-Temperature Orbital & Reciprocal Shaker

Overview

The Hanuo SHA-BA is a dual-mode water bath constant-temperature shaker engineered for precise thermal and mechanical control in life science laboratories. It integrates orbital and reciprocal oscillation within a thermostatically regulated water bath environment—enabling uniform heat transfer and consistent agitation critical for sensitive biological processes. Unlike air-jacketed shakers, the water bath design ensures superior temperature homogeneity (±0.5 °C stability) across sample vessels, minimizing thermal gradients that can compromise cell viability, enzyme kinetics, or hybridization efficiency. The device operates on a microprocessor-controlled digital interface, allowing independent programming of temperature, speed, and time—making it suitable for standardized protocols in molecular biology, microbiology, immunology, and biopharmaceutical process development.

Key Features

• Dual oscillation mode: Selectable orbital (circular) or reciprocal (linear back-and-forth) motion—or simultaneous activation—via intuitive front-panel controls.
• Stainless steel 304 water bath chamber and platform ensure long-term corrosion resistance against aqueous buffers, saline solutions, and mild reagents commonly used in cell culture and biochemical assays.
• Smooth, brushless DC motor drive delivers stable speed control from 0 to 300 rpm with no splash generation—even at maximum amplitude—preserving sample integrity and lab safety.
• 20 mm fixed amplitude optimized for compatibility with standard Erlenmeyer flasks (50–1000 mL), test tubes, and multi-well plates mounted on spring-loaded clamps.
• Programmable timer (0–120 minutes) with auto-shutdown functionality supports unattended operation during overnight incubations or timed reaction protocols.
• Digitally displayed real-time temperature and speed feedback enables traceable parameter monitoring—essential for GLP-compliant workflows and internal audit documentation.

Sample Compatibility & Compliance

The SHA-BA accommodates a wide range of vessel types—including glass and plastic conical flasks, culture tubes, and PCR strip holders—when secured using its adjustable spring clamp system. Its uniform water bath temperature distribution meets ASTM E2918-21 requirements for thermal performance validation of laboratory shaking incubators. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, the instrument’s deterministic control logic and non-volatile parameter storage support integration into validated environments when paired with qualified data logging systems. It complies with IEC 61010-1:2010 safety standards for electrical equipment used in laboratory settings.

Software & Data Management

The SHA-BA operates as a standalone unit with embedded firmware; no external PC software is required for basic operation. All setpoints (temperature, speed, duration) are retained in non-volatile memory after power cycling. For laboratories requiring electronic record retention, optional RS-232 or USB-to-serial adapters enable connection to LIMS or custom Python/Excel-based acquisition scripts. Audit trail functionality—including timestamped start/stop events and parameter changes—is achievable through third-party middleware compliant with ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate).

Applications

• Bacterial and yeast culture expansion under controlled aeration and thermal conditions.
• Enzyme kinetic assays requiring precise temperature maintenance during continuous mixing.
• Nucleic acid hybridization (e.g., Southern/Northern blotting) where uniform buffer temperature prevents probe denaturation.
• Protein solubilization and antigen-antibody binding studies in immunoassay development.
• Environmental microbiology applications such as BOD testing and microbial degradation rate analysis.
• Pharmaceutical QC procedures including dissolution profile verification and excipient compatibility screening.

FAQ

What is the temperature uniformity across the water bath?
Measured uniformity is ±0.5 °C at 37 °C and ±1.0 °C across the full operating range (ambient to 95 °C), verified per ASTM E2918 Annex A2.
Can the SHA-BA be used for sterile applications?
Yes—its stainless steel construction allows autoclaving of removable components (e.g., platform clamps); however, the main unit must remain outside sterilization chambers.
Is calibration documentation provided with the instrument?
A factory calibration certificate covering temperature and speed accuracy is available upon request (NIST-traceable references used).
Does the unit support external temperature probes?
No—the built-in Pt100 sensor is fixed and calibrated for bath-level measurement only.
What safety protections are included?
Over-temperature cutoff, motor overcurrent protection, and dry-run detection (triggered if water level falls below minimum mark) are integrated into the control circuitry.