Jasco UV-2075Plus Intelligent UV-Vis Detector

Overview

The Jasco UV-2075Plus Intelligent UV-Vis Detector is a high-performance, benchtop ultraviolet–visible absorbance detector engineered for integration into HPLC and UHPLC systems. It operates on the principle of dual-beam photometry using a high-stability deuterium/tungsten lamp source and a precision monochromator with slit-controlled bandwidth (1.0 nm nominal). The detector delivers real-time, linear absorbance measurements across a spectral range of 190–900 nm with wavelength accuracy ±1.0 nm and photometric linearity up to 2.5 AU. Designed for robust routine analysis in regulated and research environments, the UV-2075Plus features intelligent self-diagnostics, automatic lamp alignment verification, and thermal stabilization of optical components to ensure long-term baseline stability and signal reproducibility. Its compact footprint and modular interface support seamless coupling with Jasco’s LC-Net II/ADC data acquisition platform or third-party chromatography data systems (CDS) via analog output or RS-232/USB.

Key Features

• Intelligent diagnostics with automated lamp status monitoring, wavelength calibration validation, and optical path integrity checks
• Dual-beam optical architecture with real-time reference beam correction to minimize drift from lamp intensity fluctuations or solvent absorption shifts
• Programmable wavelength scanning (190–900 nm) and time-programmable wavelength switching during gradient runs
• Adjustable response time (0.1–10 s) and filter time constants optimized for both high-speed UHPLC separations and high-sensitivity trace analysis
• Integrated flow cell with 10 mm pathlength (standard), 8 µL dead volume, and pressure rating up to 40 MPa (5800 psi)
• Compliant with Jasco’s Intelligent Device Protocol (IDP), enabling plug-and-play recognition and parameter synchronization within Jasco LC networks

Sample Compatibility & Compliance

The UV-2075Plus is compatible with standard reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatographic methods. It supports aqueous, organic, and mixed mobile phases—including aggressive solvents such as THF, chloroform, and concentrated acids/bases—when used with chemically resistant flow cells (e.g., sapphire or fused silica windows). The detector meets key regulatory design expectations for analytical instrumentation: its firmware supports audit trail-enabling modes when paired with compliant CDS software; raw absorbance data are timestamped and stored in vendor-neutral formats (e.g., ASCII .dat); and hardware-level security features include password-protected method editing and user-access level configuration. While not pre-certified for 21 CFR Part 11 out-of-the-box, the system is fully compatible with validated GxP workflows when deployed with appropriate procedural controls and electronic signature configurations.

Software & Data Management

The detector interfaces natively with Jasco’s ChromNAV chromatography software (v2.2 or later) via LC-Net II/ADC controller, enabling full remote control of wavelength, gain, response time, and zero adjustment. ChromNAV provides GLP-compliant data handling—including electronic signatures, method versioning, and audit-trail generation—as well as peak integration algorithms compliant with USP , EP 2.2.46, and ASTM E260 standards. Raw absorbance signals are digitized at 20 Hz with 16-bit resolution, ensuring high-fidelity capture of sharp peaks in fast gradients. For legacy or hybrid lab environments, analog voltage output (0–1 V full scale) and TTL-compatible event triggers allow integration with non-Jasco data systems, including Agilent ChemStation, Thermo Chromeleon, and custom LabVIEW applications.

Applications

The UV-2075Plus serves critical roles across pharmaceutical QC (assay, impurity profiling, dissolution testing), environmental analysis (PAHs, pesticides, nitrate/nitrite quantification), food safety (vitamin content, preservative detection), and academic research (kinetic studies, ligand-binding assays, polymer characterization). Its wide dynamic range (0.0001–2.5 AU) and low noise floor (<±0.25 × 10⁻⁵ AU) enable reliable quantitation of low-abundance analytes without dilution or derivatization. In method development, the programmable wavelength scan function facilitates spectral library matching and peak purity assessment per ICH Q2(R2) guidelines. When configured with the optional Jasco Gecko-2000 column heater, retention time reproducibility improves significantly under temperature-sensitive separations (e.g., chiral or biomolecule analyses).

FAQ

Is this unit refurbished and tested prior to resale?
Yes. Each UV-2075Plus detector undergoes full functional verification—including lamp intensity profiling, wavelength accuracy validation, baseline noise measurement, and flow cell leak testing—per Jasco’s original factory acceptance protocol.
What documentation accompanies the instrument?
A comprehensive package includes the original English-language operation manual, calibration certificate (traceable to NIST standards), refurbishment report, and 6-month limited warranty documentation.
Can this detector be integrated into a non-Jasco HPLC system?
Yes. Analog output (0–1 V), digital trigger I/O, and RS-232/USB communication protocols enable interoperability with most commercial HPLC platforms and data systems.
Does the 6-month warranty cover parts and labor?
Yes. The warranty includes all labor, replacement parts, and return shipping for covered defects arising from materials or workmanship under normal laboratory use.
Are spare lamps and flow cells available?
Yes. Original Jasco deuterium (L6110) and tungsten (L6120) lamps, as well as standard and high-pressure flow cells (e.g., FC-2075 series), are stocked and available for immediate shipment.