KEZHE GOODSPE-1200 High-Flow Fully Automated Solid Phase Extraction System

Overview

The KEZHE GOODSPE-1200 is a high-flow, fully automated solid phase extraction (SPE) system engineered for rigorous trace-level analyte enrichment from large-volume liquid samples. It operates on positive-pressure fluid delivery—eliminating reliance on vacuum manifolds—and employs precision syringe pumps and a non-metallic 12-port rotary valve to ensure chemical compatibility across acidic, basic, and organic solvent regimes. Unlike conventional robotic SPE platforms, the GOODSPE-1200 integrates both cartridge-based (1 mL, 3 mL, 6 mL) and disk-based (47 mm SPE membrane) formats within a single, compact architecture—enabling method flexibility without hardware reconfiguration. Its design prioritizes reproducibility in regulated environments: flow stability is maintained within ±1% across the full 0–30 mL/min range via closed-loop pressure feedback, while air-assisted solvent displacement minimizes carryover between sequential elution steps. The system is validated for use with EPA-approved methods for water analysis (including EPA 508, 525.3, and 8270), supporting laboratories requiring auditable, GLP-compliant sample preparation workflows.

Key Features

• Positive-pressure driven SPE with dual syringe pumps (10 mL liquid + 10 mL air) for precise, pulse-free flow control and quantitative elution recovery
• Chemically inert wetted path constructed entirely of PTFE and borosilicate glass—compatible with aggressive solvents including concentrated HCl, NaOH, and acetonitrile
• Non-metallic 12-port rotary valve eliminates corrosion and metal ion leaching—critical for trace metal and endocrine disruptor analysis
• True multi-solvent capability: five independent solvent reservoirs enable fully programmable activation, loading, washing, and stepwise elution sequences
• Integrated air purge functionality between solvent transitions prevents cross-contamination and residual solvent interference
• No check valves or complex robotic arms—reducing failure points and enabling reliable unattended operation for up to 288 samples
• Separate aqueous and organic waste routing with dedicated discharge lines—ensuring compliance with lab safety and environmental disposal protocols
• Real-time status monitoring via Windows-based software (XP/7/8 compatible), displaying pump position, valve state, pressure readings, and active method stage

Sample Compatibility & Compliance

The GOODSPE-1200 accommodates diverse aqueous and semi-aqueous matrices—including groundwater, surface water, wastewater, fruit juices, dairy extracts, and biological fluids—without pre-filtration when used with optional inline filtration modules. Its 20 mL–20 L loading range supports both conventional pesticide residue screening and ultra-trace analysis of dioxins, PCBs, brominated flame retardants, and pharmaceutical metabolites. All hardware and software components comply with fundamental requirements for data integrity under FDA 21 CFR Part 11 (when deployed with audit-trail-enabled software configuration) and support GLP/GMP documentation standards. The system satisfies key performance criteria outlined in EPA Methods 508 (Organochlorine Pesticides and PCBs), 525.3 (Pesticides and PCBs by LC/MS/MS), and 8270 (Semivolatile Organic Compounds by GC/MS), making it suitable for contract environmental testing labs and regulatory QA/QC facilities.

Software & Data Management

The GOODSPE-1200 is controlled via KEZHE’s proprietary SPEControl software—a Windows-native application supporting method storage, recall, and parameter auto-loading. Each method file contains complete sequence definitions: valve positions, flow rates, dwell times, solvent selection logic, and air purge cycles. Software logs all operational events—including pump actuations, valve rotations, pressure excursions, and user interventions—with timestamped entries exportable as CSV for external LIMS integration. Audit trail functionality (optional configuration) records operator ID, method version, and modification history—aligning with ISO/IEC 17025 and CLIA requirements. USB interface enables direct firmware updates and method backup without network dependency.

Applications

• Environmental: Extraction of PAHs, organochlorines, PBDEs, dioxins/furans, and emerging contaminants (e.g., PFAS precursors) from 1–20 L water samples
• Food Safety: Multi-residue analysis of pesticides, veterinary drugs (quinolones, sulfonamides), mycotoxins, and plasticizers in beverages, infant formula, and plant extracts
• Pharmaceutical: Isolation of APIs and extractables from drug product leachables studies; purification of vitamins and antibiotics from fermentation broths
• Clinical & Forensic: Solid-phase extraction of illicit drugs, anesthetics, and metabolites from urine, plasma, and tissue homogenates
• Research: Coupling with GPC gel permeation chromatography (via optional GPC-SPE interface) or SpeedDry-10 parallel evaporator for integrated cleanup-concentration workflows

FAQ

Does the GOODSPE-1200 support EPA-certified methods for drinking water analysis?

Yes—it is validated for EPA Methods 508, 525.3, and 8270, with documented recovery performance across spiked matrixes including tap water, wastewater, and seawater.
Can the system handle suspended solids or turbid samples?

It does not include an integrated filter, but optional inline syringe-driven filtration modules (0.45 µm or 0.22 µm PTFE) are available to precondition particulate-laden samples prior to SPE loading.
Is method transfer from manual SPE protocols straightforward?

Yes—the software allows direct translation of manual timing and solvent volumes into automated sequences; flow rate and dwell time parameters are adjustable in 0.1 s increments for fine-tuning.
What maintenance is required for long-term reliability?

Annual calibration of syringe positioning and pressure transducers is recommended; PTFE tubing and valve rotor seals are field-replaceable with standard tools—no OEM service visit needed.
How is data security ensured during unattended runs?

All run logs are written locally to non-volatile memory; USB export requires physical access, and optional software configurations support password-protected method editing and electronic signatures per 21 CFR Part 11 Annex 11 guidelines.