KEZHE GOODSPE-2000 Automated Solid Phase Extraction System

Overview

The KEZHE GOODSPE-2000 is a fully automated, single-channel solid phase extraction (SPE) system engineered for high reproducibility, method flexibility, and unattended operation in regulated and research laboratories. It employs positive-pressure fluid delivery—rather than vacuum manifold-based aspiration—to ensure precise, consistent flow control across diverse sorbent chemistries and sample matrices. This architecture minimizes channeling, improves bed packing integrity, and delivers superior analyte recovery (>95% for typical pesticide and pharmaceutical standards under optimized conditions). Designed for compliance-critical workflows, the system supports trace-level isolation of organic contaminants from complex aqueous and semi-aqueous samples—including environmental water, food extracts, biological fluids, and pharmaceutical formulations—prior to downstream analysis by HPLC, GC, LC-MS, or ICP-MS.

Key Features

• Positive-pressure SPE platform with 10 mL syringe pump delivering programmable flow rates from 0 to 30 mL/min, enabling optimal conditioning, loading, washing, and elution steps for varied cartridge formats.
• Non-metallic 12-port rotary valve for solvent selection—chemically inert, low-dead-volume, and compatible with aggressive solvents including dichloromethane, acetonitrile, methanol, and ethyl acetate.
• Modular, motorized cartridge rack accommodating up to 40 × 1 mL, 3 mL, or 6 mL SPE cartridges simultaneously; optional custom support for 70 mL large-capacity cartridges.
• Integrated dual-needle system with independent inner/outer needle washing using dedicated rinse solvents—reducing carryover to <0.05% (validated with caffeine and atrazine).
• Programmable fraction collection into 15 mL tubes (custom volumes available); synchronized timing ensures precise eluate segmentation for multi-analyte or polarity-based fractionation.
• Over-pressure protection circuitry (setpoint: 1.2 MPa) and real-time pressure monitoring prevent column clogging damage and maintain method robustness.
• Movable cartridge stage enables full-process automation on a single platform: conditioning → sample loading → washing → drying → elution → needle wash—all without manual intervention.
• Support for stepwise elution protocols, including gradient solvent switching and timed pauses, essential for method development and multi-class contaminant isolation.

Sample Compatibility & Compliance

The GOODSPE-2000 processes liquid samples ranging from 1 to 50 mL, including filtered environmental waters, centrifuged food homogenates, protein-precipitated plasma, and diluted tissue extracts. Its PTFE- and borosilicate glass-wetted path ensures compatibility with acidic, basic, and oxidizing solvents while eliminating metal leaching—a critical requirement for trace elemental and sensitive LC-MS applications. The system conforms to key regulatory expectations for analytical instrumentation: it supports audit-trail-enabled software operation (via GS-2000 workstation), retains full method metadata (including timestamps, solvent sequences, and flow profiles), and facilitates alignment with GLP, ISO/IEC 17025, and FDA 21 CFR Part 11 requirements when deployed with appropriate IT controls and user access management.

Software & Data Management

The GS-2000 workstation software (Windows XP/7/8 compatible) provides two operational modes: Sequence Mode for routine batch processing and Batch Development Mode for iterative method optimization. Users define individual SPE methods per cartridge position—enabling parallel optimization of loading pH, wash stringency, and elution strength across 40 columns. The built-in SPE Method Library stores validated protocols with version control, parameter constraints, and instrument calibration logs. All run data—including pressure curves, solvent consumption logs, and fraction collection triggers—are timestamped and exportable in CSV or XML format. USB 2.0 interface enables secure offline data transfer; no cloud dependency or remote connectivity is required—ensuring data sovereignty in secure lab environments.

Applications

The GOODSPE-2000 is routinely deployed in accredited environmental testing labs for EPA Methods 508.1, 525.3, and 8270-compliant isolation of organochlorines, PAHs, PCBs, brominated flame retardants, and dioxins from drinking and wastewater. In food safety labs, it supports EU SANTE/11312/2021 and AOAC 2007.01 workflows for multi-residue pesticide screening, veterinary drug residues (e.g., sulfonamides, quinolones, macrolides), and mycotoxin cleanup. Pharmaceutical QC laboratories use it for residual solvent removal prior to HPLC assay, extractable/leachable studies of container-closure systems, and bioanalytical sample prep (e.g., plasma protein precipitation followed by C18 cleanup). Additional validated use cases include forensic toxicology (opioids, benzodiazepines), clinical research (vitamin D metabolites), and natural product isolation (alkaloids, flavonoids).

FAQ

What cartridge formats are supported?
The system natively accommodates 1 mL, 3 mL, and 6 mL SPE cartridges; custom fixtures for 70 mL cartridges and disk-based SPE modules are available as factory-installed options.
Can the GOODSPE-2000 be integrated with HPLC or GPC systems?
Yes—it supports offline coupling via standardized vial output. Optional HPLC-ready modules enable direct transfer of eluates to autosampler vials; GPC integration kits include fraction-triggered synchronization signals.
Is method validation documentation provided?
KEZHE supplies IQ/OQ documentation templates, pressure calibration certificates, and performance verification reports (including recovery, precision, and carryover test data) upon request.
What maintenance is required for long-term reliability?
Routine tasks include monthly valve cleaning with IPA, biannual syringe pump lubrication, and annual pressure sensor calibration—no proprietary tools are required.
Does the system comply with 21 CFR Part 11?
The GS-2000 software supports electronic signatures, audit trails, and role-based access control when configured with Windows Group Policy and domain authentication—meeting foundational Part 11 expectations for non-networked instruments.