KEZHE GOODSPE-3000B High-Throughput Automated Solid Phase Extraction System
| Brand | KEZHE SHANGHAI |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Domestic |
| Model | GOODSPE-3000B |
| Automation Level | Fully Automated |
| Channel Configuration | Modular — configurable as 1-, 2-, or 4-channel |
| Extraction Format | Cartridge-based SPE |
| Flow Rate Control | 0–30 mL/min per channel |
| Solvent Reservoirs | 8 independent solvent ports |
| Maximum Sample Capacity | 144 samples (using 1 mL cartridges) |
| Sample Loading Volume | 1–50 mL per run |
| Wetted Materials | PTFE and borosilicate glass |
Overview
The KEZHE GOODSPE-3000B is a modular, high-throughput automated solid phase extraction (SPE) system engineered for reproducible, unattended sample preparation in regulated and research-intensive laboratories. It operates on positive-pressure-driven elution—applying precisely controlled pneumatic pressure to force sample matrices and solvents through SPE cartridges at defined flow rates—thereby eliminating vacuum-induced variability and improving inter-run consistency. Unlike gravity- or vacuum-assisted systems, the GOODSPE-3000B’s pressure-regulated architecture ensures uniform bed compression across diverse sorbent chemistries (e.g., C18, silica, SCX, SAX, mixed-mode), resulting in higher analyte recovery (>92% for typical pesticide and pharmaceutical standards) and lower relative standard deviation (<3.5% RSD for replicate extractions). Its core mechanical design integrates a precision XYZ robotic arm, multi-position cartridge and fraction collector racks, and independently addressable 10 mL syringe pumps—each capable of real-time flow modulation within the 0–30 mL/min range. The system supports both sequential and parallel processing modes, enabling method development flexibility and routine batch scalability without hardware reconfiguration.
Key Features
- Modular channel architecture: Configurable as 1-, 2-, or 4-channel operation—each channel fully independent with isolated pressure control, solvent routing, and fraction collection.
- Positive-pressure delivery system: Maintains consistent flow dynamics across variable cartridge backpressures; includes overpressure cutoff (max 30 psi output) with automatic channel isolation upon blockage detection.
- Multi-solvent handling: Eight dedicated solvent reservoirs support complex multi-step protocols—including conditioning, loading, washing, and gradient elution—with non-metallic 12-port valves resistant to organic solvent corrosion.
- Comprehensive contamination control: Integrated inner/outer needle wash stations using dual-solvent rinse cycles; programmable needle depth positioning (±0.1 mm accuracy) minimizes carryover.
- In-line nitrogen-assisted concentration: Optional integrated SpeedDry-10 module enables post-elution evaporation directly in collection tubes (15 mL standard; custom volumes available).
- Dual-waste management: Separate aqueous and organic waste lines with level sensors and chemical-resistant PTFE/glass fluid paths compliant with EPA Method 525.3 and ISO 17025 traceability requirements.
- Hardware expandability: Compatible with GPC gel permeation chromatography modules, accelerated solvent extraction (ASE) offline coupling, and direct HPLC autosampler interface via standardized vial trays.
Sample Compatibility & Compliance
The GOODSPE-3000B accommodates standard SPE cartridges (1 mL, 3 mL, 6 mL) and custom large-volume formats (up to 70 mL), as well as disk-based formats (e.g., Empore™) via optional adapter kits. Its PTFE- and borosilicate-glass-wetted path ensures compatibility with aggressive solvents (e.g., DCM, THF, concentrated acids/bases) and avoids metal leaching in trace-level analyses. The system meets functional requirements for GLP and GMP environments: audit-trail-enabled software logs all user actions, method parameters, and instrument events; data files are timestamped, digitally signed, and exportable in CSV and .xlsx formats for LIMS integration. While not FDA 21 CFR Part 11–certified out-of-the-box, its architecture supports validation packages—including IQ/OQ/PQ documentation templates—for laboratories implementing electronic records under ISO/IEC 17025:2017 or USP .
Software & Data Management
The GS-3000B workstation software (Windows XP/7/8 compatible, USB 2.0 interface) provides three operational paradigms: Sequence Mode (for iterative method optimization), Batch Mode (for unattended 144-sample runs), and Custom Workflow Mode (drag-and-drop step sequencing with conditional logic). All methods store complete metadata—including cartridge type, solvent sequence, flow profile, fraction mapping, and nitrogen blow-down parameters. The software enforces sample tracking via barcode-scannable tube labels and supports dual waste stream volume monitoring with auto-shutdown alerts. Raw method files are encrypted and version-controlled; exported reports include full audit trails compliant with internal QA review protocols.
Applications
The GOODSPE-3000B serves analytical workflows requiring robust, high-volume cleanup prior to GC-MS, LC-MS/MS, or ICP-MS detection. Validated use cases include: environmental analysis of PAHs, PCBs, dioxins, and brominated flame retardants in soil/water extracts; food safety screening for pesticide residues (EU MRL-compliant), veterinary drug markers (e.g., sulfonamides, fluoroquinolones), and plasticizers (DEHP, DINP); pharmaceutical QC of extractables/leachables from packaging and residual solvents in APIs; and forensic toxicology applications including amphetamine metabolite isolation and novel psychoactive substance enrichment. Its ability to handle 1–50 mL loading volumes and execute multi-step pH-switched elutions makes it suitable for complex biological matrices (urine, plasma, tissue homogenates) in clinical and life science settings.
FAQ
What cartridge formats does the GOODSPE-3000B support?
Standard 1 mL, 3 mL, and 6 mL SPE cartridges; custom 70 mL cartridges and disk-based formats (e.g., Empore™) are supported via optional holders.
Can the system perform gradient elution?
Yes—eight independent solvent ports enable up to eight-step elution schemes with programmable flow rate transitions between steps.
Is the system compatible with regulated laboratory environments?
It supports GLP/GMP workflows through full audit trail logging, method versioning, and electronic signature-ready reporting—though formal 21 CFR Part 11 compliance requires site-specific validation.
How is cross-contamination prevented during high-throughput runs?
Via dual-solvent needle wash (inner + outer), positional repeatability of ±0.1 mm, and physical separation of aqueous/organic waste streams.
What maintenance is required for long-term reliability?
Routine replacement of PTFE seals and syringe pump tubing every 6–12 months; valve rotor lubrication and pressure sensor calibration annually per manufacturer guidelines.
