Lanbo UHPLC Multifunctional Autosampler

Overview

The Lanbo UHPLC Multifunctional Autosampler is an engineered solution for high-pressure liquid chromatography workflows requiring exceptional precision, thermal stability, and method flexibility. Designed specifically for ultra-high-performance liquid chromatography (UHPLC) systems operating at pressures up to 1300 bar, this autosampler integrates advanced fluidic architecture with a robust Peltier-based temperature management system to ensure sample integrity across extended unattended runs. Its core operation relies on positive-displacement syringe technology combined with low-dead-volume valve switching, enabling reproducible injections from sub-microliter volumes through milliliter-scale dilutions—without cross-contamination or carryover. Unlike legacy autosamplers constrained by fixed-loop geometry, the Lanbo platform supports both variable- and fixed-volume injection modes, allowing users to optimize throughput and precision based on analytical requirements rather than hardware limitations.

Key Features

• Triple-tray sample carrier accommodating up to 120 standard 1.5 mL vials (105 vials under active cooling at 4 °C)
• Injection precision of <0.1% RSD at 5 µL and 10 µL—validated per ASTM E2656 and ISO 8466-1 protocols for volumetric accuracy in analytical instrumentation
• Expandable injection volume range: 0.1–100 µL using the standard 250 µL loop; scalable to 1500 µL via field-installable large-loop modules
• Integrated Peltier-controlled chamber maintaining sample temperatures from 0 °C to 60 °C in 1 °C increments—critical for thermolabile compounds and method transfer consistency
• Vial-specific pneumatic vortex mixing and heating, independently adjustable per sample position, eliminating batch-wise homogenization artifacts
• Dilution capability with <1.0% RSD for 1:1000 dilutions (e.g., 10 µL analyte into 990 µL diluent), supporting trace-level calibration and matrix-matched spiking
• Protected method memory architecture preventing accidental overwrites—compliant with GLP/GMP data integrity principles (ALCOA+)

Sample Compatibility & Compliance

The Lanbo UHPLC Autosampler is compatible with all major UHPLC and HPLC platforms, including those conforming to ASTM D7097 (liquid chromatographic analysis of hydrocarbons), USP (chromatography), and ISO/IEC 17025:2017 (testing laboratory competence). Its fluidic pathway uses chemically inert materials (e.g., PEEK, sapphire, and stainless steel 316L) resistant to aggressive mobile phases, including high-pH buffers and organic solvents. The system meets IEC 61326-1:2013 for electromagnetic compatibility and carries CE marking for use in regulated environments. For FDA-regulated laboratories, the autosampler supports 21 CFR Part 11-compliant audit trails when paired with validated chromatography data systems (CDS) that implement electronic signatures and event logging.

Software & Data Management

Control is executed via industry-standard RS-232 serial communication, enabling bidirectional command exchange with CDS software such as Thermo Chromeleon, Waters Empower, or Agilent OpenLab. Supported remote triggers include pump start/stop, injection initiation, gradient commencement, and status polling at user-defined intervals. All method parameters—including temperature setpoints, mixing duration, injection sequence, and dilution ratios—are stored in non-volatile memory with password-protected access. Audit trail generation is handled externally by the host CDS, ensuring full traceability of method changes, injection events, and instrument state transitions—essential for inspection readiness under ISO 17025 or pharmaceutical GMP audits.

Applications

• Pharmaceutical QC/QA: Residual solvent analysis, assay validation, and forced degradation studies where injection-to-injection reproducibility directly impacts LOD/LOQ determination
• Clinical toxicology: High-throughput quantification of drugs and metabolites in plasma/serum with cold-chain preservation during autosampling
• Environmental testing: Multi-residue pesticide screening in water and soil extracts requiring precise large-volume injections (up to 1500 µL) for enhanced sensitivity
• Method development labs: Rapid evaluation of injection volume effects on peak shape, retention time stability, and column loading without hardware reconfiguration
• Reference material certification: Certified reference laboratories performing ISO Guide 35-compliant characterization where <0.1% RSD is mandated for primary calibration steps

FAQ

What is the maximum operating pressure the autosampler can withstand in-line with UHPLC systems?
The Lanbo autosampler is rated for continuous operation at system backpressures up to 1300 bar—compatible with modern sub-2-µm particle columns and high-flow-rate methods.

Does the system support partial-loop injection mode?
Yes. The autosampler supports both full-loop and partial-loop injection configurations, enabling accurate delivery of volumes smaller than the installed loop capacity without sacrificing precision.

Can the Peltier cooling system maintain 4 °C across all three trays simultaneously?
Yes. Thermal uniformity across all tray positions is maintained within ±0.5 °C at 4 °C setpoint, verified per ICH Q5C stability protocol requirements.

Is external solvent addition for dilution performed via a dedicated port or integrated pump?
Diluent addition is executed through a dedicated, independently controlled solvent channel with gravimetrically calibrated dispensing—ensuring <0.2% RSD performance independent of main flow path dynamics.

How is firmware updated, and is version history retained?
Firmware updates are applied via RS-232 using signed binary packages; version metadata and update timestamps are logged in protected memory and accessible via diagnostic commands.