Lengguang Tech UV1902 Dual-Beam Ultraviolet-Visible Spectrophotometer

Overview

The Lengguang Tech UV1902 Dual-Beam Ultraviolet-Visible Spectrophotometer is engineered for precision molecular absorption spectroscopy across the ultraviolet and visible spectral regions (190–1100 nm). Based on a rigorously balanced dual-beam optical architecture, the instrument continuously compares sample and reference beam intensities in real time—effectively compensating for source drift, detector instability, and environmental fluctuations. This design ensures high photometric stability, exceptional baseline flatness (±0.001 A), and robust long-term repeatability (wavelength reproducibility ≤0.1 nm; photometric repeatability ≤0.001 A in 0–0.5 A range). The system employs a pre-aligned Hamamatsu deuterium lamp and OSRAM tungsten-halogen lamp, optimized for spectral continuity and extended operational lifetime. Its optical path is calibrated to meet fundamental performance criteria aligned with ISO 9001 manufacturing protocols and supports routine compliance with ASTM E275, USP , and ISO/IEC 17025 method validation frameworks.

Key Features

• Dual-beam optical design with real-time reference compensation for enhanced photometric accuracy and baseline stability
• Fixed 2 nm spectral bandwidth—optimized for resolution/sensitivity balance in routine QC and research applications
• High-stability light sources: imported Hamamatsu D₂ lamp (UV) and OSRAM tungsten-halogen lamp (VIS–NIR), factory pre-aligned for rapid lamp replacement
• 6-inch backlit LCD display (320 × 240 pixels) enabling intuitive local operation without PC dependency
• Modular sample compartment with removable base plate—supports optional accessories including solid-sample holders, reflectance stages, multi-cell changers (4- and 7-position), and long-path cuvette holders (up to 100 mm)
• Comprehensive photometric range: –4 A to +4 A absorbance; transmittance from 0.0001% to 110% T
• Low-noise electronics: RMS noise ≤±0.0003 A; thermal drift ≤0.0004 A/h (after 30-min warm-up)

Sample Compatibility & Compliance

The UV1902 accommodates standard 10-mm square quartz or glass cuvettes (pathlengths 1–100 mm), as well as solid, reflective, and turbid samples via dedicated accessory modules. Its mechanical and electronic architecture complies with IEC 61010-1 safety standards for laboratory electrical equipment. While not inherently 21 CFR Part 11–compliant, its software platform supports audit-trail-enabling configurations when deployed on validated Windows systems under GLP/GMP environments. Data integrity is reinforced through timestamped calibration logging, user-access-level controls, and exportable metadata (including wavelength accuracy verification reports per ISO/IEC 17025 Annex A.3).

Software & Data Management

Controlled via Windows-based Lengguang UVWin software, the UV1902 delivers full spectral acquisition and quantitative analysis capabilities—including fixed-wavelength measurement, kinetic/time-scan mode, spectral scanning (190–1100 nm), DNA/protein quantification (A₂₆₀/A₂₈₀ ratio, ε calculations), and multi-point calibration with linear/nonlinear regression (e.g., quadratic, cubic). All raw spectra and processed results are stored in vendor-neutral CSV and XML formats. Baseline correction routines are fully configurable (zero-order, first-order, second-order polynomial), and calibration data—including dark-current subtraction and wavelength alignment maps—can be saved and reloaded per application protocol. RS232 serial interface enables integration into automated lab workflows or LIMS synchronization using standard SCPI command sets.

Applications

• Environmental analysis: Nitrate, phosphate, COD, and heavy metal speciation (e.g., Cr(VI) at 540 nm) per EPA Method 365.2 and ISO 6878
• Pharmaceutical QA/QC: Assay of active ingredients, dissolution testing, and excipient interference screening per USP and Ph. Eur. 2.2.25
• Biochemical research: Nucleic acid quantification, enzyme kinetics (e.g., NADH oxidation at 340 nm), and protein secondary structure estimation via derivative spectroscopy
• Materials science: Thin-film transmittance/absorbance profiling, bandgap estimation of semiconductors, and nanoparticle surface plasmon resonance characterization
• Food & agriculture: Pigment analysis (chlorophylls, carotenoids), antioxidant capacity (FRAP, DPPH assays), and mycotoxin detection (aflatoxin B1 at 365 nm with derivatization)
• Educational laboratories: Teaching core spectrophotometric principles—Beer–Lambert law validation, spectral deconvolution, and stray-light impact assessment

FAQ

What is the maximum pathlength supported by the UV1902 sample compartment?
The standard configuration accepts up to 100 mm pathlength cuvettes using the optional UV1900 long-path holder.
Does the instrument support automatic baseline correction during scanning?
Yes—baseline correction is performed in real time via software-controlled zero-reference referencing prior to each scan; correction profiles can be saved and reapplied.
Can the UV1902 be used for reflectance measurements?
Yes—when equipped with the optional UV1900 reflectance stage, it performs diffuse and specular reflectance analysis across the full 190–1100 nm range.
Is the lamp alignment tool included with the instrument?
No—lamp replacement is simplified by factory pre-alignment; no user alignment tools or procedures are required.
What validation documentation is provided with the system?
Each unit ships with a factory calibration certificate (traceable to NIM, China), wavelength accuracy verification report, and photometric linearity test data per ISO 6060.