Levo-Blue Large-Capacity Manual Pipette by AS ONE

Overview

The Levo-Blue Large-Capacity Manual Pipette (Model C2-3827-01) by AS ONE is an ergonomically engineered, high-precision liquid handling tool designed for repeatable, controlled dispensing of volumes up to 100 mL. Unlike conventional bulb-style pipette aids, the Levo-Blue operates on a continuous air displacement principle—enabling smooth, stepless aspiration and dispensing without repeated disconnection or reinsertion of the pipette tube. Its mechanical design eliminates reliance on disposable bulbs or battery-powered mechanisms, ensuring consistent performance in environments where electrical interference, sterilization requirements, or long-term reliability are critical. The device is optimized for use with standard glass or plastic serological pipettes (1–100 mL), supporting both routine volumetric transfers and semi-quantitative dispensing tasks in microbiology, cell culture, quality control, and preparative chemistry workflows.

Key Features

• Ergonomic lever-actuated mechanism reduces hand fatigue during extended use—especially critical for high-volume transfers requiring multiple cycles per sample.
• Autoclavable silicone tip holder (up to 121°C, 20 min) ensures compatibility with strict sterility protocols in aseptic laboratories and GMP-compliant facilities.
• Integrated hydrophobic 0.3 µm filter prevents aerosol contamination of internal components and protects users from hazardous or volatile samples.
• Universal fit design accommodates pipette tubes with outer diameters ranging from 5.0 to 8.0 mm—covering Class A, ISO 648, and ASTM E969-compliant serological pipettes.
• Calibration-stable mechanical linkage provides reproducible volume delivery across its full operational range, independent of operator technique variability.
• Modular construction allows for easy field replacement of seals, filters, and levers—minimizing downtime and extending service life beyond typical disposable aids.

Sample Compatibility & Compliance

The Levo-Blue is validated for use with aqueous solutions, buffers, alcohols, and low-viscosity organic solvents commonly encountered in life science and analytical labs. It is not recommended for highly corrosive liquids (e.g., concentrated strong acids/bases) or viscous media (>500 mPa·s) without prior validation. The autoclavable silicone interface complies with ISO 13485 manufacturing standards and supports GLP/GMP documentation requirements when paired with traceable calibration logs. While the device itself does not carry CE marking or FDA 510(k) clearance as an IVD device, its materials meet USP Class VI biocompatibility criteria, and its filter assembly conforms to ISO 8536-4 for medical-grade gas filtration.

Software & Data Management

As a fully manual, non-electronic instrument, the Levo-Blue requires no software, firmware, or data connectivity. This architecture inherently satisfies regulatory constraints related to electronic record integrity (e.g., FDA 21 CFR Part 11) and eliminates cybersecurity vulnerabilities associated with connected lab hardware. All usage records—including maintenance intervals, filter replacements, and sterilization cycles—are maintained manually via lab notebook entries or LIMS-integrated log templates. Optional accessories include serialized calibration verification kits (traceable to NIST SRM 2192) for periodic accuracy checks aligned with ISO/IEC 17025 internal audit requirements.

Applications

• Preparation of culture media and reagent dilutions in cell biology and microbiology labs.
• Transfer of wash buffers and staining solutions in histology and immunohistochemistry workflows.
• Serial dilution setup for ELISA, PCR master mix preparation, and QC testing under ISO 17025-accredited conditions.
• Controlled addition of solvents or precipitants in synthetic chemistry and purification processes.
• Environmental sampling workflows requiring sterile, single-use pipette handling without electronic dependency.
• Teaching laboratories where mechanical transparency and durability support student training in fundamental pipetting technique.

FAQ

Can the Levo-Blue be used with positive-displacement pipettes?

No—it is specifically engineered for air-displacement serological pipettes (1–100 mL). Positive-displacement systems require proprietary piston tips and are incompatible with this aid’s sealing geometry.
Is the entire unit autoclavable?

Only the silicone tip holder assembly is autoclavable. The main body housing (ABS polymer) must be cleaned using 70% ethanol or isopropanol and air-dried.
What is the expected service life of the integrated 0.3 µm filter?

Under normal use with non-volatile aqueous solutions, the filter typically lasts 6–12 months. Replacement is recommended after exposure to volatile organics, high-salt buffers, or visible particulate loading.
Does AS ONE provide calibration certificates for this model?

AS ONE supplies optional third-party calibration reports (NIST-traceable) upon request at time of order; these are not included by default but may be added to purchase orders for regulated environments.
How does the Levo-Blue compare to electronic pipette controllers in terms of accuracy?

While electronic controllers offer programmable speed profiles and digital readouts, the Levo-Blue delivers equivalent volumetric repeatability (CV < 2.5% at 100 mL) with superior mechanical robustness and zero power dependency—making it preferable for cleanrooms, biosafety cabinets, and field-deployed labs.