LUMEX Capel-105M Capillary Electrophoresis System
| Brand | LUMEX |
|---|---|
| Origin | Russia |
| Model | Capel-105M |
| CE Instrument Type | Capillary Electrophoresis (CE) |
| Voltage Range | ±1–25 kV (1 kV step, polarity auto-switching) |
| Detection Wavelength | 190–400 nm |
| Light Source | High-intensity deuterium lamp |
| Pressure Range | 0–99 mbar (1 mbar step) |
| Capillary Flushing Pressure | 1000–2000 mbar (1 mbar step) |
| Capillary Dimensions | 30–120 cm length × 50 or 75 µm ID |
| Temperature Control | Water-cooled thermostating, –10 to +30 °C (±0.1 °C stability) |
| Sample Introduction | Fully automated 20-position sampler (10 inlet + 10 outlet positions), electrokinetic & pressure-driven injection |
| Current Monitoring | 0–200 µA |
| Power Supply | 110/220 VAC, 50/60 Hz, 200 W |
| Dimensions | 500 × 500 × 500 mm |
| Weight | 25 kg |
| Software | Elforun PC-based control and data analysis suite |
Overview
The LUMEX Capel-105M Capillary Electrophoresis System is a high-performance, benchtop analytical platform engineered for precision separation and quantitative detection of ionic and polar analytes in complex matrices. It operates on the fundamental principle of capillary electrophoresis—where analytes migrate under the influence of a high-voltage electric field through a fused-silica capillary filled with an electrolyte buffer. Separation resolution is governed by differences in electrophoretic mobility (charge-to-size ratio) and, depending on mode, partitioning behavior (e.g., in micellar electrokinetic chromatography). The system integrates a stabilized UV-Vis absorbance detector with a high-output deuterium lamp and optimized optical path, enabling robust detection across 190–400 nm. Its water-cooled capillary cartridge ensures thermal homogeneity during high-field operation (up to ±25 kV), minimizing Joule heating artifacts and delivering reproducible migration times—critical for regulatory-compliant quantitation in GLP and GMP environments.
Key Features
- Fully automated 20-position sample handling system with dual-zone configuration (10 inlet + 10 outlet vials), supporting unattended batch analysis.
- Dual-mode injection: precise pressure-driven (0–99 mbar, 1 mbar resolution) and electrokinetic (1–25 kV, 1 kV step) options—enabling optimal loading for low-conductivity or high-viscosity samples.
- High-stability water-cooling system maintaining capillary temperature from –10 °C to +30 °C with ±0.1 °C accuracy—superior to air cooling in thermal uniformity and more cost-effective than cryogenic liquid systems.
- Programmable high-voltage power supply with automatic polarity reversal, real-time current monitoring (0–200 µA), and integrated safety interlocks compliant with IEC 61010-1.
- Rapid capillary exchange mechanism—capillaries (30–120 cm × 50/75 µm ID) can be replaced in under 3 minutes without optical realignment.
- UV detection module featuring a high-intensity deuterium lamp, low-stray-light optics, and digital signal processing to achieve sub-micromolar detection limits for common ions and organic acids.
Sample Compatibility & Compliance
The Capel-105M supports a broad range of separation modes—including capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary isoelectric focusing (CIEF), capillary gel electrophoresis (CGE), and isotachophoresis (ITP)—making it suitable for charged small molecules, amino acids, organic acids, inorganic cations/anions, vitamins, preservatives, pesticides, and therapeutic proteins. Its design conforms to key international standards for analytical instrumentation: ISO/IEC 17025 method validation requirements, ASTM D8085 (standard guide for CE in environmental analysis), and USP <1053> (electrophoretic methods). Data integrity features—including electronic signatures, audit trails, and user-access controls in Elforun software—support compliance with FDA 21 CFR Part 11 for regulated laboratories conducting QC testing in pharmaceutical, food, and environmental sectors.
Software & Data Management
Control and data acquisition are managed via the Elforun software suite—a Windows-based application designed specifically for capillary electrophoresis workflows. Elforun provides intuitive method setup wizards for CZE, MEKC, CIEF, and ITP; real-time electropherogram visualization; peak integration using baseline correction and second-derivative algorithms; and customizable reporting templates exportable to PDF, CSV, Excel, and XML formats. The software includes spectral scanning functionality for wavelength optimization and peak identification, as well as built-in calibration routines for voltage, pressure, and temperature parameters. All instrument actions—including voltage ramping, pressure pulses, UV wavelength selection, and temperature setpoints—are logged with timestamps and operator IDs, fulfilling ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) data governance criteria.
Applications
The Capel-105M delivers validated performance across diverse regulated and research-driven applications: environmental analysis of anions (Cl⁻, NO₃⁻, SO₄²⁻), cations (Na⁺, K⁺, Ca²⁺, Mg²⁺), herbicides, and heavy metal complexes in water and soil extracts; food safety testing for organic acids (citric, malic, tartaric), sulfites, benzoates, nitrites, caffeine, polyphenols, and adulterants (e.g., melamine); pharmaceutical QC of chiral drug enantiomers, counterions in APIs, and protein charge variants; clinical biochemistry for hemoglobin variant profiling and serum protein electrophoresis; forensic analysis of explosives residues (e.g., RDX, TNT), ink dyes, and document authenticity markers; and agricultural feed analysis for amino acid profiles and vitamin B-group quantitation.
FAQ
What separation modes does the Capel-105M support?
It supports capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC), capillary isoelectric focusing (CIEF), capillary gel electrophoresis (CGE), and isotachophoresis (ITP).
Is the system compatible with regulatory data integrity requirements?
Yes—Elforun software implements full 21 CFR Part 11 compliance, including electronic signatures, audit trails, role-based access control, and immutable raw data storage.
Can viscous or particulate-containing samples be analyzed?
Yes—pressure-assisted injection (0–99 mbar) enables reliable introduction of higher-viscosity samples; optional inline filtration or centrifugation prior to injection is recommended for particulate-laden matrices.
What maintenance is required for long-term operational stability?
Routine maintenance includes daily capillary rinsing with NaOH and water, quarterly optical alignment verification, annual calibration of HV supply and pressure transducer, and biannual replacement of deuterium lamp.
Does the system support method transfer from other CE platforms?
Yes—Elforun allows import of method parameters (voltage, temperature, buffer composition, detection wavelength) and supports standardized CE file formats for cross-platform comparability.
