LY DYCZ-20F DNA Sequencing Electrophoresis Apparatus

Overview

The LY DYCZ-20F DNA Sequencing Electrophoresis Apparatus is a high-stability vertical slab gel electrophoresis system engineered for high-resolution nucleic acid separation in molecular biology laboratories. Designed specifically for DNA sequencing applications—including Sanger-based sequencing, differential display PCR, DNA fingerprinting, and single-strand conformation polymorphism (SSCP) analysis—the DYCZ-20F employs standard polyacrylamide or denaturing urea-PAGE gel matrices to achieve sharp, reproducible band resolution under controlled electric field conditions. Its vertical configuration supports uniform current distribution across large-format gels, minimizing edge effects and thermal gradient-induced band distortion. The apparatus operates in conjunction with compatible power supplies (e.g., LY DYY-10C, DYY-12, DYY-12C, DYY-12D, and DYY-15D models), enabling precise voltage and current regulation per experimental protocol requirements.

Key Features

• Integrated vertical electrophoresis architecture with modular upper and lower buffer chambers for simplified assembly and cleaning.
• Optimized thermal management via passive convection design and strategically positioned ventilation pathways—ensures uniform temperature distribution across the gel matrix during extended runs (up to 12 h at constant voltage).
• Dual-comb compatibility: includes two 68-tooth and two 100-tooth shark-tooth sample combs (0.4 mm thickness), supporting high-density loading of sequencing ladders or multiplex PCR products.
• Large-format gel tray (450 × 340 mm) accommodates standard sequencing gels up to 0.4 mm thick, enabling parallel analysis of multiple samples with minimal cross-contamination risk.
• Ergonomic front-panel labeling on both glass plates—etched with scale markers and orientation indicators—for rapid alignment and consistent gel casting.
• Integrated buffer drain port on the lower chamber facilitates efficient buffer exchange and waste removal without disassembly.
• Comprehensive gel-casting accessories included: precision灌胶 bottle (160-0021), silicone sealing strips (143-2005), clamping system (144-0040, n=12), and dual-glass plate set (flat and concave, 142-2061/142-2062) for reliable, bubble-free polymerization.

Sample Compatibility & Compliance

The DYCZ-20F supports native and denatured polyacrylamide gels (3–20% T, 2.6–6% C), as well as agarose-polyacrylamide hybrid matrices for intermediate-resolution applications. It is routinely validated for use with common DNA sequencing chemistries (e.g., dideoxy termination, fluorescent dye-primer, and dye-terminator kits) and compatible with post-electrophoresis staining protocols (ethidium bromide, SYBR Gold, silver nitrate). While not an IVD device, the system adheres to general laboratory safety standards per ISO 13485-aligned manufacturing practices and meets CE marking requirements for electrical safety (EN 61010-1). Its mechanical design complies with GLP-relevant documentation traceability for instrument setup—buffer volume, comb type, and gel thickness are routinely recorded in lab notebooks or LIMS for audit readiness.

Software & Data Management

As a hardware-only electrophoresis platform, the DYCZ-20F does not include embedded firmware or proprietary software. However, it integrates seamlessly into digital workflows through interoperability with third-party imaging systems (e.g., Bio-Rad ChemiDoc, Azure Sapphire, UVP Biospectrum) and image analysis suites (ImageLab, Quantity One, Fiji/ImageJ). Raw gel images captured post-run support automated lane detection, molecular weight calibration, and band intensity quantification when paired with calibrated DNA ladders (e.g., pGEM DNA Markers, φX174/HaeIII digest). For regulated environments, users may implement 21 CFR Part 11-compliant electronic signatures and audit trails via validated LIMS platforms that log instrument usage parameters (date/time, operator ID, gel dimensions, comb configuration).

Applications

• DNA sequencing ladder resolution (e.g., 50–500 bp range) with sub-base-pair discrimination under optimized urea-PAGE conditions.
• SSCP analysis of point mutations in clinical or population genetics studies, leveraging conformational mobility shifts in single-stranded DNA fragments.
• Differential display profiling of mRNA expression patterns across tissue types or treatment conditions.
• Forensic DNA fingerprinting using multilocus probe hybridization or PCR-amplified STR loci.
• Quality control of synthetic oligonucleotides and CRISPR guide RNAs prior to functional validation.
• Verification of restriction enzyme digestion efficiency and cloning construct integrity.

FAQ

Is the DYCZ-20F compatible with automated gel documentation systems?

Yes—it is mechanically and dimensionally aligned with standard UV transilluminators and CCD-based gel imagers; no adapters are required.
What power supply models are certified for use with this unit?

LY DYY-10C, DYY-12, DYY-12C, DYY-12D, and DYY-15D power supplies have been validated for voltage/current output stability and safety interlock synchronization.
Can the system be used for protein electrophoresis?

While primarily designed for nucleic acids, the DYCZ-20F supports SDS-PAGE and native PAGE for proteins up to ~200 kDa when paired with appropriate gel concentrations and running buffers.
Does the apparatus include temperature monitoring or active cooling?

No—thermal regulation relies on passive dissipation; for high-voltage or long-duration runs, external recirculating chillers (e.g., PolyScience 10–30 °C units) may be connected via the 600 mm silicone tubing (included).
Are replacement parts available for long-term maintenance?

All component SKUs (e.g., 141-2031, 142-2062, 144-0040) are stocked globally through authorized LY distributors and support multi-year service life under standard laboratory use conditions.