LY DYCZ-23A Mini Vertical Electrophoresis Apparatus

Overview

The LY DYCZ-23A Mini Vertical Electrophoresis Apparatus is a compact, benchtop vertical electrophoresis system engineered for high-reproducibility SDS-PAGE and native PAGE applications in molecular biology and protein biochemistry laboratories. It operates on the principle of electrophoretic mobility separation—where charged biomolecules (primarily proteins or nucleic acids) migrate through a polyacrylamide gel matrix under the influence of a direct current electric field. The migration rate correlates inversely with molecular weight under denaturing conditions (e.g., SDS-PAGE), enabling precise band resolution and comparative analysis. Designed for single-gel operation, the DYCZ-23A features an integrated upper and lower buffer chamber architecture that minimizes electrolyte volume while maintaining stable thermal dissipation—critical for preventing gel distortion during extended runs. Its small footprint and passive thermal management make it especially suitable for teaching labs, QC environments with limited space, and preliminary screening workflows where rapid setup and minimal reagent consumption are prioritized.

Key Features

• Compact vertical electrophoresis configuration optimized for single 100 × 80 mm gel plates—ideal for low-throughput validation, student training, and pilot-scale protein characterization.
• Dual-buffer tank design (upper and lower chambers) reduces total buffer volume to 350 mL, lowering operational cost and waste generation without compromising electrophoretic uniformity.
• Modular comb and spacer system supports two standard gel thicknesses: 1.0 mm (15-well comb) for high-resolution protein separation and 1.5 mm (10-well comb) for higher sample loading capacity.
• Transparent acrylic housing enables real-time visual monitoring of buffer level, electrode integrity, and bubble formation during electrophoresis.
• Stainless-steel electrodes with corrosion-resistant plating ensure long-term conductivity stability and consistent field distribution across the gel surface.
• Tool-free assembly/disassembly of glass plates, spacers, and sealing components facilitates rapid cleaning and cross-contamination prevention between runs.

Sample Compatibility & Compliance

The DYCZ-23A accommodates standard vertical polyacrylamide gels used in SDS-PAGE, gradient gels, and discontinuous buffer systems (e.g., Laemmli). It is compatible with common protein stains (Coomassie Brilliant Blue, silver stain) and downstream immunoblotting protocols. While not certified for regulated GMP/GLP environments, its mechanical consistency and documented performance parameters align with routine laboratory practices referenced in ISO/IEC 17025-accredited testing workflows. The unit complies with IEC 61010-1 safety standards for electrical laboratory equipment, including protection against electric shock and thermal hazards during continuous operation at ≤200 V and ≤500 mA.

Software & Data Management

The DYCZ-23A is a standalone hardware platform with no embedded microcontroller or digital interface; therefore, it does not require proprietary software or firmware updates. All operational parameters—including voltage, current, and run duration—are controlled externally via compatible DC power supplies (e.g., LY DYY-6C or third-party constant-voltage/current sources). This analog architecture ensures full compatibility with existing lab infrastructure and eliminates concerns related to software validation, cybersecurity, or FDA 21 CFR Part 11 compliance for audit trails. Users maintain complete control over experimental documentation through standard lab notebooks or LIMS-integrated electronic records.

Applications

• Qualitative and semi-quantitative analysis of protein expression profiles in cell lysates, tissue homogenates, and purified fractions.
• Verification of recombinant protein purity and molecular weight after affinity chromatography or ion-exchange purification.
• Assessment of protein degradation, aggregation, or post-translational modifications under varying storage or treatment conditions.
• Teaching core electrophoretic principles—including buffer chemistry, gel polymerization kinetics, and electroosmotic effects—in undergraduate biochemistry and molecular biology courses.
• Preliminary screening of antibody-antigen interactions via native PAGE prior to structural characterization.

FAQ

What power supply is required to operate the DYCZ-23A?
A DC power supply capable of delivering 0–600 V and 0–500 mA is recommended. Constant voltage mode is typically used for SDS-PAGE (e.g., 120–200 V), while constant current may be applied for native gels.
Can the DYCZ-23A accommodate gradient gels?
Yes—the 100 × 80 mm gel format supports both homogeneous and linear acrylamide gradient gels (e.g., 8–20%) when cast using standard gradient mixers and casting stands.
Is cooling support integrated into the apparatus?
No active cooling module is built-in; however, the low buffer volume (350 mL) and open-tank geometry promote passive heat dissipation. For extended high-voltage runs, external recirculating chillers or ice baths may be employed.
Are replacement parts available internationally?
All consumables—including combs, spacers, glass plates, and sealing components—are cataloged under LY part numbers (e.g., 141-2311, 142-2312) and distributed globally through authorized LY partners and life science OEM distributors.
Does the system meet CE or UL certification requirements?
The DYCZ-23A conforms to IEC 61010-1:2010 for laboratory electrical equipment safety. CE marking is applied for units exported to the European Economic Area; UL listing is not applicable as it is not marketed for North American retail channels under UL 61010-1.