LY DYCZ-24DN Mini Dual Vertical Electrophoresis Apparatus
| Brand | LY |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Authorized Distributor |
| Product Category | Domestic |
| Model | DYCZ-24DN |
| Instrument Type | Protein Electrophoresis System |
| Gel Plate Dimensions (L × W) | 83 × 75 mm |
| Sample Combs | 10- and 15-well, 1.0 mm and 1.5 mm thickness (standard), 0.75 mm (optional) |
| External Dimensions (L × W × H) | 140 × 100 × 150 mm |
| Buffer Capacity | 400 mL |
| Weight | 1 kg |
Overview
The LY DYCZ-24DN Mini Dual Vertical Electrophoresis Apparatus is a compact, benchtop vertical electrophoresis system engineered for high-resolution SDS-PAGE and native protein separation. Based on the fundamental principle of electrophoretic mobility—where charged polypeptides migrate through a polyacrylamide gel matrix under an applied electric field—the DYCZ-24DN enables reproducible, parallel analysis of two independent gels within a single unit. Its miniaturized architecture maintains full functional integrity while minimizing bench space and buffer consumption, making it especially suitable for teaching laboratories, QC workflows with limited throughput, and preliminary method development in research settings. The system operates within standard DC power supply ranges (typically 100–300 V, 10–100 mA), compatible with widely available laboratory power supplies meeting IEC 61010-1 safety requirements.
Key Features
- True in-situ gel casting enabled by precision-molded polycarbonate gel former—eliminates gel leakage, air bubble entrapment, and misalignment during polymerization.
- Optically transparent polycarbonate main chamber allows real-time monitoring of electrophoretic migration without disassembly.
- Dual-gel capability: Simultaneous operation of two independent 83 × 75 mm gels using shared buffer reservoirs and unified electrode configuration.
- Interchangeable comb system supporting 10- and 15-well formats with standardized thickness options (1.0 mm and 1.5 mm); 0.75 mm combs available for high-resolution low-volume applications.
- Integrated safety design: Lid-activated circuit interruption ensures automatic power cutoff upon opening; flexible, abrasion-resistant leads reduce strain-induced failure.
- Modular glass plate assembly—including concave plates pre-bonded with spacer strips (1.0/1.5 mm), flat plates, sealing gaskets, and removable electrode inserts—enables rapid reconfiguration and long-term dimensional stability.
- Zero-buffer-leak geometry achieved via precision-machined sealing surfaces and compression-fit gasket placement, ensuring consistent conductivity and thermal homogeneity across runs.
Sample Compatibility & Compliance
The DYCZ-24DN accommodates standard acrylamide/bis-acrylamide gels (8–20% T), gradient gels, and Tricine- or Bis-Tris-based systems for low-MW protein resolution. It supports common sample loading volumes (10–30 µL per well) and is compatible with Coomassie Blue, silver, and fluorescent staining protocols. While not certified to ISO/IEC 17025, the apparatus conforms to general laboratory equipment safety standards (GB/T 29302–2012 equivalent to IEC 61010-1) and supports GLP-aligned documentation when paired with validated power supplies and staining workflows. Its open architecture permits integration into SOPs compliant with USP , ISO 13845, and basic FDA 21 CFR Part 11 data integrity principles—particularly when used with timestamped imaging and manual logbook entries.
Software & Data Management
The DYCZ-24DN is a hardware-only electrophoresis platform and does not include embedded firmware or proprietary software. All operational parameters—including voltage, current, run duration, and ambient temperature—are manually controlled and recorded externally. This design aligns with laboratories prioritizing instrument transparency, auditability, and minimal validation overhead. Users typically pair the system with third-party gel documentation tools (e.g., ImageLab, AlphaView SA, or Fiji/ImageJ) for band quantification, molecular weight calibration, and comparative densitometry. Raw gel images, annotated run logs, and calibration curves can be archived in structured directories compliant with ALCOA+ (Attributable, Legible, Contemporaneous, Original, Accurate) principles.
Applications
- SDS-PAGE for protein purity assessment and subunit analysis in biopharmaceutical QC labs.
- Native PAGE for oligomeric state evaluation of enzymes and membrane protein complexes.
- Western blotting pre-electrophoresis preparation, including transfer compatibility verification.
- Teaching modules on electrophoretic theory, gel polymerization kinetics, and buffer ion dynamics.
- Preliminary screening of expression constructs, cleavage efficiency, or post-translational modifications.
- Method optimization for 2D-PAGE first-dimension compatibility and narrow-range gradient gels.
FAQ
Can the DYCZ-24DN accommodate gradient gels?
Yes—its uniform glass plate spacing and leak-proof chamber support stable gradient polymerization using standard gradient mixers and stacking gels.
Is the electrode assembly field-replaceable?
Yes—the platinum-coated stainless-steel electrodes are modular and designed for tool-free replacement without recalibration.
What power supply specifications are recommended?
A programmable DC power supply delivering 0–300 V / 0–100 mA with constant voltage or constant current mode is required; ripple ≤ 0.5% RMS is advised for optimal band sharpness.
Does the system support temperature-controlled runs?
No—it lacks active cooling or heating elements; however, its low buffer volume (400 mL) and polycarbonate insulation enable stable ambient-temperature operation for ≤90 min runs in climate-controlled labs.
Are spare parts such as glass plates and combs available separately?
Yes—LY provides full OEM part numbers (e.g., 142-2445A, 141-2446) for individual component replenishment, ensuring long-term serviceability and protocol consistency across instrument lifecycles.
