Metler Toledo E4 XLS+ 8-Channel Electronic Pipette
| Brand | Rainin |
|---|---|
| Origin | USA |
| Distributor Type | Authorized Distributor |
| Origin Category | Imported |
| Model | E4 XLS+ |
| Instrument Type | Multichannel Pipette |
| Automation Level | Electronic Pipette |
| Channels | 8 |
| Volume Range | 1–300 µL |
| Maximum Capacity | 300 µL |
| Accuracy | ±1.0% at 0.2 µL, ±0.8% at 1.6 µL |
Overview
The Mettler Toledo E4 XLS+ 8-Channel Electronic Pipette is an engineered solution for high-throughput, precision liquid handling in regulated and research-intensive laboratory environments. Built on Rainin’s proven electronic pipetting platform, it operates via microprocessor-controlled piston displacement using a high-resolution stepper motor (4,000 steps per full stroke), ensuring reproducible volumetric delivery across all eight channels. Its core measurement principle relies on calibrated air displacement with real-time positional feedback and automatic zero-point recalibration after each aspiration/dispense cycle—eliminating cumulative drift and maintaining traceable accuracy per ISO 8655 and ASTM E1154 standards. Designed specifically for applications demanding strict adherence to GLP and GMP workflows, the E4 XLS+ integrates hardware-level security features with audit-ready data logging, making it suitable for QC labs, biopharma process development, and academic core facilities where procedural integrity and instrument accountability are non-negotiable.
Key Features
- 8-channel electronic pipetting with synchronized channel actuation and independent tip ejection force control
- Volume range of 1–300 µL with certified accuracy per ISO 8655:2022 (±1.0% at 0.2 µL; ±0.8% at 1.6 µL) and precision ≤0.5% CV
- Onboard microprocessor governing piston speed, acceleration, and dwell time—enabling programmable aspiration/dispense profiles
- Ergonomic ambidextrous design with low-force tip ejection (silicone-damped mechanism), contoured finger hooks, and glove-compatible joystick interface
- Full-color backlit display with rotary navigation dial and tactile joystick for intuitive menu navigation and rapid protocol selection
- Automatic cycle counting and AutoPace mode for consistent multi-well plate dispensing without manual repetition
- Zero-point auto-recalibration after every pipetting cycle to maintain long-term metrological stability
Sample Compatibility & Compliance
The E4 XLS+ is compatible with universal low-retention and filtered pipette tips meeting ISO 8655-compliant dimensions, including Rainin LTS and PureSpeed™ tips optimized for viscous, foaming, or temperature-sensitive biological samples (e.g., proteins, oligonucleotides, cell lysates). It supports routine use with aqueous buffers, organic solvents (within material compatibility limits), and diluted acids/bases. From a regulatory standpoint, the device complies with IEC 61326-1 (EMC), IEC 61010-1 (safety), and is validated for use under FDA 21 CFR Part 11-compliant environments when paired with Rainin’s LiteTouch™ software and secure networked service portal. All configuration changes, service alerts, and usage logs are digitally signed and tamper-evident—supporting full audit trails required by GLP, GMP, and ISO/IEC 17025-accredited laboratories.
Software & Data Management
The E4 XLS+ stores up to 20 user-defined protocols directly in non-volatile memory, including complex sequences such as serial dilutions, multi-step dispense routines, and repeat dispensing with variable step volumes. Protocols can be locked or password-protected to prevent unauthorized modification—critical for SOP enforcement. Service records, total actuation cycles, and calibration status are stored onboard and exportable via USB or Rainin’s cloud-connected service portal. Firmware updates and remote diagnostics are performed securely through encrypted channels. The integrated PureSpeed™ function enables optimized aspiration/dispense kinetics for high-molecular-weight biomolecules, reducing shear stress and improving recovery yields—particularly valuable in nucleic acid purification and protein expression workflows.
Applications
- High-density ELISA and immunoassay plate setup (96-/384-well)
- Automated cDNA synthesis and qPCR master mix preparation
- Cell culture passaging and media supplementation across multi-well formats
- Serial dilution series generation for IC50/EC50 determination
- Genomic library normalization and NGS sample indexing
- GMP-compliant reagent aliquoting and reference standard preparation
FAQ
Does the E4 XLS+ support regulatory compliance for FDA 21 CFR Part 11?
Yes—when used with password-protected protocol locking, digital signature-enabled service logs, and audit-trail-capable software interfaces, it meets foundational requirements for electronic record integrity.
Can I adjust aspiration and dispense speeds independently?
Yes—the microprocessor allows granular control over piston velocity, acceleration ramp, and post-aspiration/dispense pause duration for each step in a protocol.
Is tip compatibility limited to Rainin-branded consumables?
No—it accepts any ISO-standard conical tip (LTS or universal fit), though optimal performance with low-retention or high-viscosity samples is achieved using Rainin PureSpeed™ or LTS tips.
How often does the E4 XLS+ require recalibration?
It performs automatic zero-point recalibration after every pipetting cycle; however, scheduled verification per ISO 8655 is recommended every 3–6 months depending on usage intensity and criticality of application.
What happens if the battery fails during a protocol?
All active protocols and settings are retained in non-volatile memory; upon recharge or battery replacement, the device resumes operation with no data loss or configuration reset.
