METTLER TOLEDO EVA V Coulometric Karl Fischer Moisture Titrator with InMotion KF Heated Autosampler

Overview

The METTLER TOLEDO EVA V is a high-precision volumetric Karl Fischer titrator engineered for trace to macro-level moisture quantification in challenging matrices—including hygroscopic, thermally labile, viscous, or chemically reactive samples. When coupled with the InMotion™ KF heated autosampler, the system implements a closed-loop, temperature-controlled sample handling architecture that minimizes ambient moisture ingress and enables reproducible thermal conditioning prior to titration. The instrument operates on the fundamental electrochemical principle of Karl Fischer titration: iodine generated *in situ* via controlled electrolysis (coulometric mode) or delivered volumetrically (volumetric mode) reacts stoichiometrically with water in the sample dissolved in KF solvent. The EVA V platform is configured exclusively for volumetric determination—utilizing dual-piston burettes with active pressure compensation and real-time reagent consumption monitoring—to ensure metrological traceability and long-term stability across extended analytical campaigns.

Key Features

• Volumetric titration architecture with dual-piston burette delivering ±0.001 mL precision and automatic reagent calibration tracking
• InMotion™ KF heated autosampler with 26-position carousel, independently programmable vial heating (ambient to 150 °C), and inert gas purging (N₂ or dry air) to suppress atmospheric moisture interference
• One Click™ workflow automation: simultaneous method assignment across heterogeneous sample sets—including variable sample mass, solvent volume, heating profile, and endpoint criteria—without manual reconfiguration
• LabX® software integration enabling full 21 CFR Part 11 compliance: role-based access control, electronic signatures, immutable audit trail, and automated report generation with raw data archiving
• Compact footprint (≤25 cm bench space required for full 26-sample capacity), optimized for high-throughput QC labs with spatial constraints
• Real-time titration curve visualization with adaptive endpoint detection (dynamic drift compensation and slope-based termination)

Sample Compatibility & Compliance

The EVA V + InMotion KF configuration supports solid, liquid, paste, and powder samples—particularly those prone to side reactions (e.g., aldehydes, ketones, basic amines) or low solubility (e.g., polymers, pharmaceutical excipients, battery electrolytes). Pre-heating under inert atmosphere facilitates controlled water release from bound or crystalline hydrates while suppressing decomposition. The system conforms to ASTM D6304 (standard test method for determination of water in petroleum products), ISO 8534 (rubber—determination of moisture content), USP (water determination), and European Pharmacopoeia 2.5.12. All hardware and software components are validated for GLP and GMP environments, with documented IQ/OQ/PQ protocols available upon request.

Software & Data Management

LabX® serves as the centralized control and data governance layer. It enforces method versioning, prevents unauthorized parameter modification during run execution, and automatically logs all user actions, instrument events, and environmental conditions (e.g., ambient humidity, reagent batch ID, titrant concentration). Raw titration curves, calculated moisture values (ppm or % w/w), statistical summaries (RSD, confidence intervals), and certificate-of-analysis templates are exportable in PDF/A, CSV, or XML formats. Data integrity is preserved through cryptographic hashing and time-stamped digital signatures aligned with FDA 21 CFR Part 11 requirements.

Applications

• Pharmaceutical: Water content verification in APIs, lyophilized powders, and final dosage forms per ICH Q5C guidelines
• Chemicals & Polymers: Residual moisture in polyamide, polyacrylonitrile, and silicone elastomers affecting polymerization kinetics and shelf life
• Battery Materials: Quantification of trace H₂O in LiPF₆ electrolytes and cathode precursors—critical for SEI layer formation and cell safety
• Food & Feed: Compliance testing for moisture limits in powdered dairy, infant formula, and pet food per AOAC 992.17
• Electronics: Moisture screening in solder pastes, conformal coatings, and encapsulation resins to prevent delamination and corrosion

FAQ

Can the InMotion KF autosampler handle samples requiring precise thermal ramping?
Yes—the carousel supports multi-step temperature profiles per vial, including hold times, ramp rates, and cooling phases, all programmable within LabX®.
Is method transfer possible between EVA V systems across different laboratories?
Fully supported via LabX® method export/import with embedded calibration history, reagent definitions, and instrument configuration metadata.
How is reagent stability monitored during unattended overnight runs?
The system logs real-time titrant consumption, calculates remaining reagent volume, and triggers alerts when concentration deviation exceeds ±0.5% of nominal value.
Does the system support non-aqueous solvents other than standard KF methanol-based reagents?
Yes—validated compatibility with chloroform, ethylene glycol monomethyl ether, and imidazole-based solvents for highly acidic or basic samples.
What validation documentation is supplied with the instrument?
Factory-issued IQ/OQ documentation, metrological traceability certificates for burette calibration, and a complete 21 CFR Part 11 implementation report.