Polytech SPE432 Automated Solid Phase Extraction System
| Brand | Polytech |
|---|---|
| Origin | Beijing, China |
| Manufacturer Type | Manufacturer |
| Origin Category | Domestic |
| Model | SPE 432 |
| Pricing | Upon Request |
Overview
The Polytech SPE432 Automated Solid Phase Extraction System is an engineered solution for high-throughput, method-robust sample preparation in environmental, food safety, and regulatory laboratories. Designed around dual-pump fluid handling architecture—comprising a precision syringe pump for low-volume (1–50 mL) applications and a high-capacity peristaltic metering pump for large-volume (100 mL–2 L) extractions—the SPE432 enables seamless integration of diverse sample matrices within a single run sequence. Its operational principle relies on controlled vacuum or positive-pressure-driven liquid flow through commercially available SPE cartridges (e.g., C18, Florisil, HLB, SAX), facilitating selective analyte retention, matrix removal, and elution under programmable solvent gradients. This system meets the evolving demands of multi-matrix monitoring programs—including those outlined in China’s 2024 Pilot Program for Emerging Contaminants Monitoring—where analytical workflows must accommodate wide-ranging sample volumes across water, soil, sediment, air particulate filters, and biological tissues.
Key Features
- Dual independent pump system: Syringe pump (±1% accuracy at 1–50 mL) and peristaltic metering pump (linear flow control up to 2 L/min) ensure precise loading, washing, and elution across volume scales.
- Modular cartridge manifold with reusable column sealing gaskets minimizes carryover and eliminates cross-contamination during sequential large-volume processing.
- Universal tube compatibility: Accepts standard 12–15 mL and 50 mL centrifuge tubes without transfer steps; integrates natively with Polytech nitrogen evaporators for downstream concentration.
- Single-sequence flexibility: Small- and large-volume samples may be processed consecutively without hardware reconfiguration or cartridge holder replacement.
- Open-cartridge design supports all major commercial SPE sorbents (e.g., Waters Oasis®, Agilent Bond Elut®, Thermo HyperSep™) and accommodates custom-packed columns.
- Upgradable fluid path: Optional PFA/PEEK tubing kits enable trace-level analysis of perfluoroalkyl substances (PFAS), including PFOS and PFOA, in compliance with HJ 1333–2023 and HJ 1334–2023.
- Robust mechanical architecture with sealed stepper-motor actuators and corrosion-resistant stainless-steel chassis ensures long-term reliability in high-humidity or solvent-rich lab environments.
Sample Compatibility & Compliance
The SPE432 processes aqueous, semi-aqueous, and mildly organic extracts from environmental solid and liquid matrices—including surface water, groundwater, wastewater, seawater, soil leachates, sediment pore water, and air filter extracts. It supports validated methods aligned with GB/T 5750.8–2023 (drinking water organics), GB/T 5750.9–2023 (pesticides), GB/T 5750.10–2023 (disinfection by-products), HJ 805–2016 (PAHs), HJ 921–2017 (organochlorine pesticides), HJ 922–2017 (PCBs), and HJ 1189–2021 (organophosphorus pesticides). System operation adheres to GLP principles, with full audit trail capability via optional software modules compliant with FDA 21 CFR Part 11 requirements for electronic records and signatures.
Software & Data Management
The embedded control interface provides intuitive method programming—including stepwise vacuum/pressure control, solvent selection, dwell times, and fraction collection logic—with real-time status monitoring and error logging. Exportable CSV logs record time-stamped event sequences (e.g., “Cartridge 3 – Load complete @ 14:22:07”), enabling retrospective QC review. Optional Polytech SPE Manager v3.2 software adds method library management, user role-based access control, electronic signature support, and automated report generation compatible with LIMS integration via ASTM E1578-compliant data export protocols.
Applications
- Environmental monitoring of persistent organic pollutants (POPs), including PFAS, PAHs, PCBs, and legacy pesticides in multi-phase environmental media.
- Pre-concentration and cleanup of drinking water and wastewater samples prior to LC-MS/MS or GC-MS analysis.
- Soil and sediment extract purification for regulatory compliance testing under national environmental standards.
- Method development and validation for emerging contaminant workflows requiring variable sample volumes and mixed-sorbent strategies.
- High-volume screening programs where throughput, reproducibility, and minimal manual intervention are critical performance criteria.
FAQ
Does the SPE432 support both vacuum- and pressure-driven SPE modes?
Yes—it operates using programmable positive-pressure nitrogen gas delivery or vacuum manifold control, selectable per method step.
Can I use third-party SPE cartridges with this instrument?
Absolutely—the system accepts all standard 1-, 3-, 6-, and 12-mL commercial cartridges without adapters.
Is method validation documentation available for regulatory submissions?
Polytech provides IQ/OQ documentation templates and application notes aligned with ISO/IEC 17025 and CNAS-CL01 requirements.
What maintenance intervals are recommended for routine operation?
Syringe pump calibration every 6 months; peristaltic pump tubing replacement every 1,000 cycles or 6 months, whichever occurs first.
How does the system prevent cross-contamination during large-volume runs?
Each cartridge position features an independently sealable gasket assembly that isolates flow paths and prevents residual carryover between samples.
