PUREN PAS-III Multifunctional Autosampler for HPLC and GC Systems

Overview

The PUREN PAS-III Multifunctional Autosampler is an engineered solution for high-throughput, precision liquid handling in chromatographic laboratories. Designed to integrate seamlessly with HPLC, UHPLC, GC, and IC systems, it operates on a robust XYZ three-axis motion architecture that ensures repeatable positioning accuracy of ±0.2 mm across a compact footprint of 360 × 280 × 260 mm (W × D × H). Unlike conventional single-function autosamplers, the PAS-III implements a unified fluidic and mechanical platform capable of performing three distinct operational roles—autosampling, sample dilution, and standard preparation—within a single instrument chassis. Its core actuation relies on a high-resolution stepper motor-driven syringe pump (5 mL capacity), enabling precise aspiration and dispensing down to 10 µL per injection. The system supports both standalone operation via integrated touchscreen interface and full remote control through industry-standard communication protocols (RS-232, USB, and optional Ethernet), ensuring compatibility with major chromatography data systems (CDS) including Thermo Chromeleon, Agilent OpenLab, Waters Empower, and Shimadzu LabSolutions.

Key Features

• Triple-function architecture: simultaneous autosampling, programmable dilution, and standard solution preparation without hardware reconfiguration
• Dual independent sample trays: enables concurrent sample loading and analysis—reducing downtime and increasing laboratory throughput
• Integrated needle wash station with dual-path solvent delivery: internal lumen flush and external surface rinse using separate wash solvents, minimizing carryover and cross-contamination
• Modular needle assembly with interchangeable long/short gauge needles: accommodates diverse vial geometries (e.g., 12 × 32 mm, 15 × 45 mm, and deep-well plates), eliminating vacuum-induced sampling errors
• Enclosed operational chamber with corrosion-resistant coating (electroless nickel + PTFE composite): enhances operator safety and extends service life in aggressive solvent environments (e.g., acetonitrile, TFA, HFIP)
• User-configurable security: password-protected startup and method editing, supporting role-based access control in regulated environments
• Real-time status visualization: live display of current vial position, wash cycles completed, aspiration/dilution steps executed, and remaining repeat injections

Sample Compatibility & Compliance

The PAS-III accepts standard 12 mm and 15 mm diameter screw-thread or crimp-top vials, as well as 96-well microplates (SBS format). Its programmable dilution module supports serial dilution, matrix spiking, and internal standard addition with volumetric accuracy traceable to ISO/IEC 17025-accredited calibration procedures. The instrument complies with essential design principles outlined in FDA 21 CFR Part 11 for electronic records and signatures, supporting audit trail generation, user authentication, and method version control when operated under validated CDS environments. It also meets mechanical safety requirements per IEC 61010-1 and electromagnetic compatibility standards per EN 61326-1, making it suitable for GLP and GMP-compliant QC laboratories conducting routine pharmaceutical, environmental, or food testing.

Software & Data Management

The embedded touchscreen interface provides intuitive method setup—including injection sequence definition, wash protocol selection, dilution factor specification, and repeat injection scheduling—with no external PC required. For integration into enterprise workflows, the PAS-III supports ASCII-based command sets compatible with third-party scripting (Python, LabVIEW) and native drivers for leading CDS platforms. All method parameters, execution logs, and error events are timestamped and stored locally on non-volatile memory; optional network connectivity enables centralized log export to LIMS or ELN systems. Audit trails include operator ID, timestamp, parameter changes, and critical event flags (e.g., needle clog detection, tray misalignment), fulfilling ALCOA+ data integrity expectations.

Applications

• Routine quality control of APIs and finished pharmaceutical products per USP and EP 2.2.46
• Environmental analysis of PFAS, pesticides, and PAHs in water and soil extracts using EPA Methods 525.3, 8081B, and 8270
• Food safety testing for mycotoxins, preservatives, and sweeteners under EU Regulation (EC) No 401/2006
• Method development workflows requiring iterative dilution series or multi-level calibration curve generation
• High-volume clinical toxicology screening where reproducible low-volume injections (<20 µL) are critical for LC-MS/MS sensitivity

FAQ

Does the PAS-III support unattended overnight operation?
Yes—the system includes automatic power-on self-test, tray homing verification, and fault-tolerant error recovery. If a vial is missing or needle blockage occurs, it logs the event and continues with the next valid position.
Can I use non-PUREN syringes or needles?
While third-party 10–500 µL syringes and 22–26G stainless steel needles may physically fit, PUREN recommends factory-calibrated consumables to maintain volumetric accuracy and ensure warranty coverage.
Is method validation documentation available?
Instrument qualification protocols (IQ/OQ) and performance verification templates are provided upon request, aligned with ASTM E2500 and ICH Q2(R2) guidelines.
What maintenance intervals are recommended?
Daily: needle wash solvent level check and waste bottle emptying. Quarterly: syringe pump lubrication and XYZ rail cleaning. Annually: full metrological recalibration by authorized service center.
How does the PAS-III handle viscous or particulate-containing samples?
The dual-path wash system and programmable pre-rinse volume mitigate clogging risk. For suspensions, users may enable “slow aspirate” mode and specify post-aspiration dwell time to ensure complete draw-up.